Strategies to minimize variability and bias associated with manual pipetting in ligand binding assays to assure data quality of protein therapeutic quantification

2010 ◽  
Vol 53 (3) ◽  
pp. 623-630 ◽  
Author(s):  
Kinnari Pandya ◽  
Chad A. Ray ◽  
Laura Brunner ◽  
Jin Wang ◽  
Jean W. Lee ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Data Quality ◽  
Binding Assays ◽  
Protein Therapeutic

Data quality in drug discovery: the role of analytical performance in ligand binding assays

2015 ◽  
Vol 29 (9) ◽  
pp. 847-865 ◽  
Author(s):  
Hermann Wätzig ◽  
Imke Oltmann-Norden ◽  
Franziska Steinicke ◽  
Hassan A. Alhazmi ◽  
Markus Nachbar ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Ligand Binding ◽  
Data Quality ◽  
Analytical Performance ◽  
Binding Assays

scholarly journals Critical reagents for ligand-binding assays: process development methodologies to enable high-quality reagents

Bioanalysis ◽  
2022 ◽  
Author(s):  
Caroline Kittinger ◽  
Jared Delmar ◽  
Lisa Hewitt ◽  
Rebecca Holcomb ◽  
Christopher Jones ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Process Development ◽  
Process Conditions ◽  
Generation Process ◽  
Binding Assays ◽  
Performance Control ◽  
Development Approach ◽  
Immunogenicity Assays ◽  
The Impact

Development of biotherapeutics require pharmacokinetic/pharmacodynamic (PK/PD) and immunogenicity assays that are frequently in a ligand-binding assay (LBA) format. Conjugated critical reagents for LBAs are generated conjugation of the biotherapeutic drug or anti-drug molecule with a label. Since conjugated critical reagent quality impacts LBA performance, control of the generation process is essential. Our perspective is that process development methodologies should be integrated into critical reagent production to understand the impact of conjugation reactions, purification techniques and formulation conditions on the quality of the reagent. In this article, case studies highlight our approach to developing process conditions for different molecular classes of critical reagents including antibodies and a peptide. This development approach can be applied to the generation of future conjugated critical reagents.

Designing Information Product (IP) Maps On the Process of Data Processing and Academic Information

2017 ◽  
Vol 4 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Diana Effendi
Keyword(s):  
Data Quality ◽  
Data Management ◽  
Information Management ◽  
Information Quality ◽  
Quality Data ◽  
Management Approach ◽  
Quality Of Data ◽  
Information Product ◽  
Academic Activities

Information Product Approach (IP Approach) is an information management approach. It can be used to manage product information and data quality analysis. IP-Map can be used by organizations to facilitate the management of knowledge in collecting, storing, maintaining, and using the data in an organized. The  process of data management of academic activities in X University has not yet used the IP approach. X University has not given attention to the management of information quality of its. During this time X University just concern to system applications used to support the automation of data management in the process of academic activities. IP-Map that made in this paper can be used as a basis for analyzing the quality of data and information. By the IP-MAP, X University is expected to know which parts of the process that need improvement in the quality of data and information management.   Index term: IP Approach, IP-Map, information quality, data quality. REFERENCES[1] H. Zhu, S. Madnick, Y. Lee, and R. Wang, “Data and Information Quality Research: Its Evolution and Future,” Working Paper, MIT, USA, 2012.[2] Lee, Yang W; at al, Journey To Data Quality, MIT Press: Cambridge, 2006.[3] L. Al-Hakim, Information Quality Management: Theory and Applications. Idea Group Inc (IGI), 2007.[4] “Access : A semiotic information quality framework: development and comparative analysis : Journal ofInformation Technology.” [Online]. Available: http://www.palgravejournals.com/jit/journal/v20/n2/full/2000038a.html. [Accessed: 18-Sep-2015].[5] Effendi, Diana, Pengukuran Dan Perbaikan Kualitas Data Dan Informasi Di Perguruan Tinggi MenggunakanCALDEA Dan EVAMECAL (Studi Kasus X University), Proceeding Seminar Nasional RESASTEK, 2012, pp.TIG.1-TI-G.6.

scholarly journals Response Behavior and Quality of Survey Data: Comparing Elderly Respondents in Institutions and Private Households

2021 ◽  
pp. 004912412199553
Author(s):  
Jan-Lucas Schanze
Keyword(s):  
Data Quality ◽  
The Elderly ◽  
Response Behavior ◽  
Quality Of Data ◽  
Social Surveys ◽  
Private Households ◽  
Confounding Variables ◽  
Health Related ◽  
Survey Interviews

An increasing age of respondents and cognitive impairment are usual suspects for increasing difficulties in survey interviews and a decreasing data quality. This is why survey researchers tend to label residents in retirement and nursing homes as hard-to-interview and exclude them from most social surveys. In this article, I examine to what extent this label is justified and whether quality of data collected among residents in institutions for the elderly really differs from data collected within private households. For this purpose, I analyze the response behavior and quality indicators in three waves of Survey of Health, Ageing and Retirement in Europe. To control for confounding variables, I use propensity score matching to identify respondents in private households who share similar characteristics with institutionalized residents. My results confirm that most indicators of response behavior and data quality are worse in institutions compared to private households. However, when controlling for sociodemographic and health-related variables, differences get very small. These results suggest the importance of health for the data quality irrespective of the housing situation.

scholarly journals The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kenichi Kamata ◽  
Kenji Mizutani ◽  
Katsuya Takahashi ◽  
Roberta Marchetti ◽  
Alba Silipo ◽  
...  
Keyword(s):  
Immune System ◽  
Sialic Acid ◽  
Ligand Binding ◽  
Steric Hindrance ◽  
Sequence Similarity ◽  
Binding Assays ◽  
Subunit Interface ◽  
Nmr Techniques ◽  
And Control ◽  
Asialo Gm1

AbstractSeviL is a recently isolated lectin found to bind to the linear saccharides of the ganglioside GM1b (Neu5Ac$$\alpha$$ α (2-3)Gal$$\beta$$ β (1-3)GalNAc$$\beta$$ β (1-4)Gal$$\beta$$ β (1-4)Glc) and its precursor, asialo-GM1 (Gal$$\beta$$ β (1-3)GalNAc$$\beta$$ β (1-4)Gal$$\beta$$ β (1-4)Glc). The crystal structures of recombinant SeviL have been determined in the presence and absence of ligand. The protein belongs to the $$\beta$$ β -trefoil family, but shows only weak sequence similarity to known structures. SeviL forms a dimer in solution, with one binding site per subunit, close to the subunit interface. Molecular details of glycan recognition by SeviL in solution were analysed by ligand- and protein-based NMR techniques as well as ligand binding assays. SeviL shows no interaction with GM1 due to steric hindrance with the sialic acid branch that is absent from GM1b. This unusual specificity makes SeviL of great interest for the detection and control of certain cancer cells, and cells of the immune system, that display asialo-GM1.

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