Fatty acids-based microemulsion liquid chromatographic determination of multiple caffeoylquinic acid isomers and caffeic acid in honeysuckle sample

2019 ◽  
Vol 171 ◽  
pp. 22-29 ◽  
Author(s):  
Li-Hong Ye ◽  
Li-Jing Du ◽  
Jun Cao
Keyword(s):  
Fatty Acids ◽  
Caffeic Acid ◽  
Chromatographic Determination ◽  
Caffeoylquinic Acid ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Gas-Liquid Chromatographic Determination of C22 Fatty Acids for the Certification of Low Erucic Acid Varieties of Rapeseeds

1976 ◽  
Vol 59 (4) ◽  
pp. 855-858
Author(s):  
Jean P Barrette
Keyword(s):  
Fatty Acids ◽  
Erucic Acid ◽  
Methyl Esters ◽  
Chromatographic Determination ◽  
Detector Response ◽  
Check Sample ◽  
Liquid Chromatographic Determination ◽  
Low Erucic Acid ◽  
Liquid Chromatographic

Abstract A rapid gas-liquid chromatographic (GLC) procedure was developed for the determination of C22 fatty acids (mostly erucic acid) in rapeseeds containing less than 5% erucic acid. The method involves an oil extraction from a representative sample of rapeseeds, a vigorous boron trifluoride-methanol transesterification of the oil to methyl esters, and analysis by GLC with the aid of an electronic integrator. Six laboratories participated in a monthly check sample program. By adjusting the GLC detector response with 2 synthetic reference standard mixtures containing 1.0 and 3.0% methyl behenate, interlaboratory agreement within 0.3% C22 was consistently obtained.

Mass Spectrometric Identification and Gas-Liquid Chromatographic Determination of 2-Chloroethyl Esters of Fatty Acids in Spices and Foods

1979 ◽  
Vol 62 (4) ◽  
pp. 786-791
Author(s):  
David L Heikes ◽  
Kenneth R Griffitt
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Content ◽  
Chromatographic Determination ◽  
Food Samples ◽  
Esterification Reaction ◽  
Gas Liquid Chromatography ◽  
Mass Spectrometric Identification ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract The 2-chloroethyl esters of 5 fatty acids have been identified in spice and food samples by gas-liquid chromatography-mass spectrometry (GLC/MS). Twenty-four spice samples were analyzed for the 2-chloroethyl esters of fatty acids by AOAC official multiple residue pesticide procedures using CLC with microcoulometric detection. The esters of capric, lauric, myristic, palmitic, and linoleic acids have been identified at levels up to 1400 ppm. 2-Chloroethyl linoleate was the most abundant ester in all samples. Several foods analyzed by the same procedures showed levels of 2-chloroethyl linoleate as high as 35 ppm. Recoveries from fortified samples ranged from 84 to 98% for the various esters. A method using an acid-catalyzed esterification reaction was developed to rapidly determine the fatty acid content of these spices. CLC analysis with microcoulometric detection was used. Recoveries from fortified samples ranged from 92 to 110%. After 2 spice samples found to be free of 2-chloroethyl esters were fumigated with ethylene oxide, the level of 2-chloroethyl linoleate reached 77 ppm. All levels of 2-chloroethyl esters were confirmed by GLC/MS.

Gas-Liquid Chromatographic Determination of Sucrose Fatty Acid Esters

1983 ◽  
Vol 66 (4) ◽  
pp. 1050-1052
Author(s):  
Taizo Tsuda ◽  
Hiroshi Nakanishi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Methyl Esters ◽  
Chromatographic Determination ◽  
Fatty Acid Esters ◽  
Liquid Chromatographic Determination ◽  
Sucrose Fatty Acid Esters ◽  
Liquid Chromatographic ◽  
Acid Esters

Abstract A method was developed for gas-liquid chromatographic determination of sucrose fatty acid esters as TMS derivative of sucrose and methyl esters of fatty acids. Sucrose fatty acid esters were completely degraded to sucrose and fatty acids in alkaline ethanol overnight at 25°C. Sucrose was derivatized with pyridine, trimethylchlorosilane, and N-trimethylsilylimidazole and the sucrose TMS derivative was determined on a 2% OV-17 column. Fatty acids were extracted with ethyl ether, methylated with BF3-methanol complex at 65°C, and determined on a 2% DEGS + 0.5% H3PO4 column. This method was applied to selected sucrose fatty acid esters. For example, sucrose and fatty acids derived from 50 mg sample F20 were 10.6-11.0 and 38.1-39.0 mg, respectively. Total amounts were 48.7-50.0 mg with a standard deviation of 0.4 (n = 6).

scholarly journals Rapid Liquid Chromatographic Determination of Fatty Acids as 2-Nitrophenylhydrazine Derivatives

1996 ◽  
Vol 79 (2) ◽  
pp. 493-497 ◽  
Author(s):  
Hiroshi Miwa ◽  
Magobei Yamamoto
Keyword(s):  
Fatty Acids ◽  
Fats And Oils ◽  
Chromatographic Determination ◽  
Trans Isomers ◽  
Liquid Chromatograph ◽  
Quantitative Accuracy ◽  
Direct Derivatization ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Direct derivatization of saponified fats and oils with 2-nitrophenylhydrazine hydrochloride produced corresponding fatty acid hydrazides without timeconsuming sample cleanup and/or extraction steps. Hydrazides were injected directly into a liquid chromatograph. Improved isocratic separation was achieved within only 22 min for 29 saturated and mono- and polyunsaturated fatty acids (C8:0- C22:6), including cis-trans isomers and doublebond positional isomers. The technique has good quantitative accuracy while reducing analysis time, cost, and labor.

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