Bactericidal action of ferulic acid with ultraviolet-A light irradiation

Emergence of more virulent forms of human pathogenic bacteria with multi drug resistance is a serious global issue and requires alternative control strategies. The current study was focused to investigate the antibacterial and antibiofilm potential of ferulic acid grafted chitosan (CFA) against Listeria monocytogenes (LM), Pseudomonas aeruginosa (PA), and Staphylococcus aureus (SA). The present result showed that CFA at 64 µg/mL concentration exhibit bactericidal action against LM and SA (>4 log reduction) and bacteriostatic action against PA (<2 log CFU) within 24 h of incubation. Further studies based on propidium iodide uptake assay, measurement of material released from the cell, and electron microscopic analysis revealed that the bactericidal action of CFA was due to the altered membrane integrity and permeability. CFA dose-dependently inhibited biofilm formation (52-89% range), its metabolic activity (30.8-75.1% range) and eradicated mature biofilms, and reduced viability (71-82% range) of the test bacteria. Also, the swarming motility of LM was differentially affected at sub-MIC concentration of CFA. In the present study, the ability of CFA to kill and alter the virulence production in human pathogenic bacteria will insight a new scope for the application of these biomaterials in healthcare to effectively treat bacterial infections.

Download Full-text

Inactivating foodborne pathogens in apple juice by combined treatment with fumaric acid and ultraviolet-A light, and mechanisms of their synergistic bactericidal action

Food Microbiology ◽

10.1016/j.fm.2019.103387 ◽

2020 ◽

Vol 87 ◽

pp. 103387 ◽

Cited By ~ 5

Author(s):

Min-Jin Jeon ◽

Jae-Won Ha

Keyword(s):

Foodborne Pathogens ◽

Fumaric Acid ◽

Apple Juice ◽

Combined Treatment ◽

Bactericidal Action ◽

Ultraviolet A

Download Full-text

Antibacterial and Biofilm Modulating Potential of Ferulic Acid-Grafted Chitosan against Human Pathogenic Bacteria

International Journal of Molecular Sciences ◽

10.3390/ijms19082157 ◽

2018 ◽

Vol 19 (8) ◽

pp. 2157 ◽

Cited By ~ 7

Author(s):

Chakradhar Dasagrandhi ◽

Seulki Park ◽

Won-Kyo Jung ◽

Young-Mog Kim

Keyword(s):

Ferulic Acid ◽

Bacterial Infections ◽

Pathogenic Bacteria ◽

Membrane Integrity ◽

Microscopic Analysis ◽

Multi Drug Resistance ◽

Bactericidal Action ◽

Electron Microscopic ◽

Iodide Uptake ◽

Human Pathogenic Bacteria

The emergence of more virulent forms of human pathogenic bacteria with multi-drug resistance is a serious global issue and requires alternative control strategies. The current study focused on investigating the antibacterial and antibiofilm potential of ferulic acid-grafted chitosan (CFA) against Listeria monocytogenes (LM), Pseudomonas aeruginosa (PA), and Staphylococcus aureus (SA). The result showed that CFA at 64 µg/mL concentration exhibits bactericidal action against LM and SA (>4 log reduction) and bacteriostatic action against PA (<2 log colony forming units/mL reduction) within 24 h of incubation. Further studies based on propidium iodide uptake assay, measurement of material released from the cell, and electron microscopic analysis revealed that the bactericidal action of CFA was due to altered membrane integrity and permeability. CFA dose dependently inhibited biofilm formation (52–89% range), metabolic activity (30.8–75.1% range) and eradicated mature biofilms, and reduced viability (71–82% range) of the test bacteria. Also, the swarming motility of LM was differentially affected at sub-minimum inhibitory concentration (MIC) concentrations of CFA. In the present study, the ability of CFA to kill and alter the virulence production in human pathogenic bacteria will offer insights into a new scope for the application of these biomaterials in healthcare to effectively treat bacterial infections.

Download Full-text

Photostability of TiO2-Coated Wool Fibers Exposed to Ultraviolet B, Ultraviolet A, and Visible Light Irradiation

Autex Research Journal ◽

10.2478/aut-2019-0055 ◽

2019 ◽

Vol 0 (0) ◽

Author(s):

Qi Tang ◽

Hui Zhang ◽

Ye Han ◽

Dou Wang ◽

Hailiang Wu

Keyword(s):

Thermal Stability ◽

Visible Light ◽

Visible Light Irradiation ◽

High Intensity ◽

Ultraviolet B ◽

Light Irradiation ◽

Ultraviolet A ◽

Orientation Degree ◽

X Ray ◽

Wool Fibers

Abstract This study is to investigate the role of the coating of TiO2 nanoparticles deposited on wool fibers against high-intensity ultraviolet B (UVB), ultraviolet A (UVA), and visible light irradiation. The properties of tensile and yellowness and whiteness indices of irradiated TiO2-coated wool fibers are measured. The changes of TiO2-coated wool fibers in optical property, thermal stability, surface morphology, composition, molecular structure, crystallinity, and orientation degree are characterized using diffuse reflectance spectroscopy, thermogravimetric analysis, scanning electronic microscopy, energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction techniques. Experimental results show that the tensile properties of anatase TiO2-coated wool fibers can be degraded under the high-intensity UVB, UVA, and visible light irradiation for a certain time, resulting in the loss of the postyield region of stress–strain curve for wool fibers. The coating of TiO2 nanoparticles makes a certain contribution to the tensile property, yellowness and whiteness indices, thermal stability, and surface morphology of wool fibers against high-intensity UVB, UVA, and visible light irradiation. The high-intensity UVB, UVA, and visible light can result in the photo-oxidation deterioration of the secondary structure of TiO2-coated wool fibers to a more or less degree. Meanwhile, the crystallinity and orientation degree of TiO2 coated wool fibers decrease too.

Download Full-text