scholarly journals Retinal degeneration rat model: A study on the structural and functional changes in the retina following injection of sodium iodate

2019 ◽  
Vol 196 ◽  
pp. 111514 ◽  
Author(s):  
Avin Ee-Hwan Koh ◽  
Hiba Amer Alsaeedi ◽  
Munirah binti Abd Rashid ◽  
Chenshen Lam ◽  
Mohd Hairul Nizam Harun ◽  
...  
Keyword(s):  
Retinal Degeneration ◽  
Rat Model ◽  
Sodium Iodate ◽  
Functional Changes

scholarly journals Ocular safety assessment of sodium iodate in cynomolgus monkeys

2017 ◽  
Vol 1 ◽  
pp. 239784731769637 ◽  
Author(s):  
Chang-Ning Liu ◽  
Qinghai Peng ◽  
David W Yates ◽  
Wenhu Huang ◽  
Heather Devantier ◽  
...  
Keyword(s):  
Retinal Degeneration ◽  
Cell Function ◽  
Retinal Pigment ◽  
Vehicle Control ◽  
Retinal Toxicity ◽  
Circulating Microrna ◽  
Large Animal ◽  
Sodium Iodate ◽  
Cynomolgus Monkeys ◽  
Functional Changes

Although sodium iodate (NaIO3)-induced retinal injury model has been widely used in rodents, its application in large animal species has encountered variation in retinal toxicity. NaIO3 induced retinal degeneration and functional changes in sheep, but not in swine. In monkeys, administration of NaIO3 via a carotid artery affected only the cell function of ipsilateral retinal pigment epithelium. The aim of the present study was to identify the dosage and route of NaIO3 administration resulting in morphologic and functional retinal changes in cynomolgus monkeys. Separate groups of animals received NaIO3 intravenously in three different dosing paradigms. Vehicle control animals received phosphate-buffered saline. At selected time points following dosing, flash electroretinograms (ERGs) were recorded followed by necropsy. The eyes were examined microscopically post-necropsy and the levels of circulating microRNA-183 cluster were evaluated in the blood samples collected on days 1, 4, and 5 postdose. A statistically significant reduction in both scotopic a-wave and scotopic and photopic b-wave signals ( p < 0.05) were observed between the ERG signals acquired from NaIO3-treated and vehicle control animals, coupled with time-dependent elevations in plasma miR-183 cluster. Mild to moderate retinal degeneration was observed in the outer layer of the retina, which correlated well with the functional and clinical observations. There were no statistically significant differences in scotopic oscillatory potentials. These findings suggest that intravenous injection of sublethal NaIO3 markedly damaged the cone and rod photoreceptors both functionally and morphologically, and plasma miR-183 reflected the retinal toxicity in those animals with moderate retinal damage.

Retinal blood vessels are damaged in a rat model of NMDA-induced retinal degeneration

2010 ◽  
Vol 485 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Kaori Ueda ◽  
Tsutomu Nakahara ◽  
Maya Hoshino ◽  
Asami Mori ◽  
Kenji Sakamoto ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Retinal Degeneration ◽  
Rat Model ◽  
Retinal Blood Vessels

Hyperpolarized 129Xe Magnetic Resonance Spectroscopy in a Rat Model of Bronchopulmonary Dysplasia

2021 ◽  
Author(s):  
Jordan David Fliss ◽  
Brandon Zanette ◽  
Yonni Friedlander ◽  
Siddharth Sadanand ◽  
Andras A Lindenmaier ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Gas Exchange ◽  
Magnetic Resonance Spectroscopy ◽  
Bronchopulmonary Dysplasia ◽  
Rat Model ◽  
Resonance Spectroscopy ◽  
Gas Exchange Parameters ◽  
Functional Changes ◽  
Non Invasive

Premature infants often require mechanical ventilation and oxygen therapy which can result in bronchopulmonary dysplasia (BPD), characterized by developmental arrest and impaired lung function. Conventional clinical methods for assessing the prenatal lung are not adequate for the detection and assessment of long-term health risks in infants with BPD, highlighting the need for a non-invasive tool for the characterization of lung microstructure and function. Theoretical diffusion models, like the Model of Xenon Exchange (MOXE), interrogate alveolar gas exchange by predicting the uptake of inert Hyperpolarized (HP) 129Xe gas measured with HP 129Xe magnetic resonance spectroscopy (MRS). To investigate HP 129Xe MRS as a tool for non-invasive characterization of pulmonary microstructural and functional changes in vivo, HP 129Xe gas exchange data were acquired in an oxygen exposure rat model of BPD that recapitulates the fewer and larger distal airways and pulmonary vascular stunting characteristics of BPD. Gas exchange parameters from MOXE, including airspace mean chord length (L­m), apparent hematocrit in the pulmonary capillaries (HCT), and pulmonary capillary transit time (tx), were compared with airspace mean axis length and area density (MAL and ρ­A) and percentage area of tissue and air (PTA and PAA) from histology. L­m was significantly larger in the exposed rats (p=0.003) and correlated with MAL, ρ­A, PTA, and PAA (0.59<|ρ|<0.66 and p<0.05). Observed increase in HCT (p=0.012) and changes in tx are also discussed. These findings support the use of HP 129Xe MRS for detecting fewer, enlarged distal airways in this rat model of BPD, and potentially in humans.

scholarly journals Lung and Intestine: A Specific Link in an Ulcerative Colitis Rat Model

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Yuan Liu ◽  
Xin-Yue Wang ◽  
Xue Yang ◽  
Shan Jing ◽  
Li Zhu ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Lung Tissue ◽  
Rat Model ◽  
Close Association ◽  
Trinitrobenzene Sulfonic Acid ◽  
Microvascular Endothelium ◽  
Functional Changes ◽  
Liver And Kidney ◽  
Embryonic Origin ◽  
Endothelial Growth Factor

Background. To investigate the link and mechanisms between intestine and lung in the ulcerative colitis (UC) rat model.Materials and Methods. We used the UC rat model by immunological sensitization combined with local 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) in 50% ethanol enema, observed dynamically animal general state and body weight, examined the histological and functional changes in the colon, lung, liver, and kidney tissues, and detected microvascular endothelium response towards inflammation characterized with the expression of iNOS, TXB2, P-selectin, ICAM-1, and vascular endothelial growth factor A (VEGF-A) in the colon and lung tissue.Results. Pulmonary function results suggested ventilator disorder, and pathological findings showed interstitial pneumonia. There were no significant changes in the liver and kidney function and histopathology. The colon and lung tissue iNOS, TXB2, P-selectin, ICAM-1, and VEGF-A expression of the model rats was significantly higher than the normal rats at both time points.Conclusions. Our study is the first to demonstrate the close association between the large intestine and lung in the immune-TNBS-ethanol-induced UC rat model. Different organs and tissues with the same embryonic origin may share the same pathological specificities in a disease. The present study provided a new way of thinking for pathological changes in clinical complex diseases manifested with multiorgan damage.

scholarly journals Course of Sodium Iodate–Induced Retinal Degeneration in Albino and Pigmented Mice

2017 ◽  
Vol 58 (4) ◽  
pp. 2239 ◽  
Author(s):  
Guy Chowers ◽  
Matan Cohen ◽  
Devora Marks-Ohana ◽  
Shelly Stika ◽  
Ayala Eijzenberg ◽  
...  
Keyword(s):  
Retinal Degeneration ◽  
Sodium Iodate
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