Cell adherence efficacy of probiotic Pediococcus pentosaceus GS4 (MTCC 12683) and demonstrable role of its surface layer protein (Slp)

2020 ◽  
Vol 226 ◽  
pp. 103894
Author(s):  
Vinay Dubey ◽  
Alok Kumar Mishra ◽  
Asit Ranjan Ghosh
Keyword(s):  
Surface Layer ◽  
Cell Adherence ◽  
Pediococcus Pentosaceus ◽  
Surface Layer Protein

scholarly journals Surface-Layer Protein A (SlpA) Is a Major Contributor to Host-Cell Adherence of Clostridium difficile

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e78404 ◽  
Author(s):  
Michelle M. Merrigan ◽  
Anilrudh Venugopal ◽  
Jennifer L. Roxas ◽  
Farhan Anwar ◽  
Michael J. Mallozzi ◽  
...  
Keyword(s):  
Surface Layer ◽  
Clostridium Difficile ◽  
Host Cell ◽  
Protein A ◽  
Major Contributor ◽  
Cell Adherence ◽  
Surface Layer Protein

Structural analysis of the surface-layer protein of spirillum serpens by high-resolution electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 738-739
Author(s):  
W. H. Wu ◽  
R. M. Glaeser
Keyword(s):  
Electron Microscopy ◽  
Surface Layer ◽  
Structural Analysis ◽  
High Resolution ◽  
Low Dose ◽  
High Resolution Electron Microscopy ◽  
Optical Diffraction ◽  
Surface Layer Protein ◽  
Morphological Unit ◽  
Resolution Electron

Spirillum serpens possesses a surface layer protein which exhibits a regular hexagonal packing of the morphological subunits. A morphological model of the structure of the protein has been proposed at a resolution of about 25 Å, in which the morphological unit might be described as having the appearance of a flared-out, hollow cylinder with six ÅspokesÅ at the flared end. In order to understand the detailed association of the macromolecules, it is necessary to do a high resolution structural analysis. Large, single layered arrays of the surface layer protein have been obtained for this purpose by means of extensive heating in high CaCl2, a procedure derived from that of Buckmire and Murray. Low dose, low temperature electron microscopy has been applied to the large arrays.As a first step, the samples were negatively stained with neutralized phosphotungstic acid, and the specimens were imaged at 40,000 magnification by use of a high resolution cold stage on a JE0L 100B. Low dose images were recorded with exposures of 7-9 electrons/Å2. The micrographs obtained (Fig. 1) were examined by use of optical diffraction (Fig. 2) to tell what areas were especially well ordered.

Prediction and assessment of effective state of machinery surface layer at science intensive technologies use

2018 ◽  
Vol 2018 (8) ◽  
pp. 44-48
Author(s):  
В. Бутенко ◽  
V. Butenko
Keyword(s):  
Surface Layer ◽  
Quality Index ◽  
Working Capacity ◽  
Material Quality ◽  
Layer Material ◽  
Operation Characteristics

The assessment indices of surface layer effective state in machinery subjected to different ways of finishing are considered. The role of the dislocation restructurization process in the formation of operation characteristics of surface layer material quality and prediction of its working capacity is shown. A generalized quality index of multi-component functional layers formed on operation surfaces of machinery is described.

Role of suspended matter in controlling beryllium-7 (7Be) in the Black Sea surface layer

2021 ◽  
pp. 103513
Author(s):  
Dmitrii A. Kremenchutskii ◽  
Gennady F. Batrakov ◽  
Illarion I. Dovhyi ◽  
Yury A. Sapozhnikov
Keyword(s):  
Surface Layer ◽  
Black Sea ◽  
Suspended Matter ◽  
Sea Surface ◽  
The Black Sea

scholarly journals Active immunization of hamsters againstClostridium difficileinfection using surface-layer protein

2008 ◽  
Vol 52 (2) ◽  
pp. 207-218 ◽  
Author(s):  
Déirdre B. Ní Eidhin ◽  
Julie B. O'Brien ◽  
Matthew S. McCabe ◽  
Verónica Athié-Morales ◽  
Dermot P. Kelleher
Keyword(s):  
Surface Layer ◽  
Active Immunization ◽  
Surface Layer Protein

Effects of short-term administration of Pediococcus pentosaceus on physiological characteristics, inflammation and intestinal microecology in mice

2021 ◽  
Author(s):  
Shumao Cui ◽  
Jie Jiang ◽  
Bowen Li ◽  
R. Paul Ross ◽  
Catherine Stanton ◽  
...  
Keyword(s):  
Physiological Characteristics ◽  
Short Term ◽  
Pediococcus Pentosaceus ◽  
Term Administration

The role of Pediococcus pentosaceus in gastrointestinne has received considerable attention in recent decades. This study aimed to investigate the effects of short-term administration of P. pentosaceus on physiological characteristics,...

scholarly journals toxB Gene on pO157 of Enterohemorrhagic EscherichiacoliO157:H7 Is Required for Full Epithelial Cell Adherence Phenotype

2001 ◽  
Vol 69 (11) ◽  
pp. 6660-6669 ◽  
Author(s):  
Ichiro Tatsuno ◽  
Masanori Horie ◽  
Hiroyuki Abe ◽  
Takeyoshi Miki ◽  
Kozo Makino ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Bacterial Infection ◽  
Intestinal Epithelium ◽  
Secreted Proteins ◽  
Cell Adherence ◽  
Full Adherence ◽  
Infection Study ◽  
Phenotype Expression

ABSTRACT Adherence of enterohemorrhagic Escherichia coli (EHEC) to the intestinal epithelium is critical for initiation of a bacterial infection. An in vitro infection study previously indicated that EHEC bacteria initially adhere diffusely and then proliferate to develop MC, a process that is mediated by various secreted proteins, such as EspA, EspB, EspD, Tir, and intimin, as well as other putative adherence factors. In the present study, we investigated the role of a large 93-kb plasmid (pO157) in the adherence of O157:H7 (O157Sakai) and found the toxB gene to be involved in the full adherence phenotype. A pO157-cured strain of O157Sakai (O157Cu) developed microcolonies on Caco-2 cells; however, the number of microcolonies was lower than that of O157Sakai, as were the production and secretion levels of EspA, EspB, and Tir. Introduction of a mini-pO157 plasmid (pIC37) composed of thetoxB and ori regions restored full adherence capacity to O157Cu, including production and secretion of the proteins. In contrast, introduction of a pO157 mutant possessingtoxB::Km into O157Cu could not restore the full adherence phenotype. Expression of truncated versions of His-tagged ToxB also promoted EspB production and/or secretion by O157Cu. These results suggest that ToxB contributes to the adherence of EHEC to epithelial cells through promotion of the production and/or secretion of type III secreted proteins.

Role of Surface Band Gap Widening in Cu(In, Ga)(Se, S)2 Thin-Films for the Photovoltaic Performance of ZnO/CdS/Cu(In, Ga)(Se, S)2 Heterojunction Solar Cells

2003 ◽  
Vol 763 ◽  
Author(s):  
U. Rau ◽  
M. Turcu
Keyword(s):  
Thin Films ◽  
Surface Layer ◽  
Solar Cells ◽  
Fermi Level ◽  
Band Gap ◽  
Photovoltaic Performance ◽  
Heterojunction Solar Cells ◽  
Fermi Level Pinning ◽  
Device Efficiency

AbstractNumerical simulations are used to investigate the role of the Cu-poor surface defect layer on Cu(In, Ga)Se2 thin-films for the photovoltaic performance of ZnO/CdS/Cu(In, Ga)Se2 heterojunction solar cells. We model the surface layer either as a material which is n-type doped, or as a material which is type-inverted due to Fermi-level pinning by donor-like defects at the interface with CdS. We further assume a band gap widening of this layer with respect to the Cu(In, Ga)Se2 bulk. This feature turns out to represent the key quality of the Cu(In, Ga)Se2 surface as it prevents recombination at the absorber/CdS buffer interface. Whether the type inversion results from n-type doping or from Fermi-level pinning is only of minor importance as long as the surface layer does not imply a too large number of excess defects in its bulk or at its interface with the normal absorber. With increasing number of those defects an n-type layer proofs to be less sensitive to material deterioration when compared to the type-inversion by Fermi-level pinning. For wide gap chalcopyrite solar cells the internal valence band offset between the surface layer and the chalcopyrite appears equally vital for the device efficiency. However, the unfavorable band-offsets of the ZnO/CdS/Cu(In, Ga)Se2 heterojunction limit the device efficiency because of the deterioration of the fill factor.

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