Comparative proteomic profiling reveals specific adaption of Vibrio anguillarum to oxidative stress, iron deprivation and humoral components of innate immunity

Introduction: Hibernation is a natural molecular adaptation to extreme environmental conditions with important implications for perioperative organ protection. Hypothesis: We hypothesized that the hibernator cardioprotective phenotype is accompanied by altered expression of innate immune pattern-recognition receptors (PRRs) and inflammatory pathways. Methods: LV myocardium, peripheral blood monocytes (PBMC), and plasma were collected from rat, summer AGS, and winter AGS after sham, 3h or 24h ischemia/reperfusion (I/R). Results: Plasma troponin I detection confirmed greater I/R injury in rat compared to AGS (Fig 1). Proteomic profiling of LV myocardium detected multiple differences including higher expression of MyD88 dependent toll-like receptors (TLR) in rat compared to AGS; average levels of TLRs were 1.8 fold higher in rat compared with hibernating AGS. Comparison of PBMCs from rat, summer, and hibernating AGS revealed increased PRR expression and cytokine production in the rat compared to the AGS, along the TLR3/TICAM (Fig2), TLR/MyD88, and AIM2/inflammasome axes. Conclusions: Compared to AGS, rats experience robust inflammasome activation in response to I/R as evidenced by >30-fold increases in AIM2 and Caspase 1. Hibernation state differences in innate immunity exist, including reduced expression of PRRs(TLR1,3, and AIM2); additionally signaling via TLR 3 and 4 is greatly dampened in winter AGS due to nearly absent expression of TICAM1. Circulating immune effector cells in winter AGS have an abrogated response to DAMPs compared to cells from summer AGS or rat, as evidenced by reduced cytokine production.

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Mtor-Dependent Oxidative Stress Regulates Oxldl-Induced Trained Innate Immunity In Human Monocytes

Atherosclerosis ◽

10.1016/j.atherosclerosis.2019.06.072 ◽

2019 ◽

Vol 287 ◽

pp. e25

Author(s):

Y. Sohrabi ◽

L. Schnack ◽

S. Lagache ◽

J. Waltenberger ◽

H. Reinecke ◽

...

Keyword(s):

Oxidative Stress ◽

Innate Immunity ◽

Human Monocytes

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Comparative effect of bovine buttermilk, whey, and lactoferrin on the innate immunity receptors and oxidative status of intestinal epithelial cells.

Biochemistry and Cell Biology ◽

10.1139/bcb-2020-0121 ◽

2020 ◽

Author(s):

Berta Buey ◽

Andrea Bellés ◽

Eva Latorre ◽

Inés Abad ◽

María Dolores Pérez ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Innate Immunity ◽

Epithelial Cells ◽

Milk Fat ◽

Functional Foods ◽

Intestinal Epithelial Cells ◽

Protein Carbonyl ◽

Intestinal Epithelial ◽

Milk Fat Globule

Milk contains active molecules with important functional properties as the defensive proteins; among them are the whey protein lactoferrin and proteins of the milk fat globule membrane (MFGM) present in buttermilk. The aim of this study has been to investigate the effect of lactoferrin, whey and buttermilk as modulators of intestinal innate immunity and oxidative stress on intestinal epithelial cells, to evaluate its potential use for the development of functional foods. Innate immune Toll-like receptors (TLR2, TLR4, and TLR9) mRNA expression, lipid peroxidation (MDA+4-HDA) and protein carbonyl levels were analyzed in enterocyte-like Caco-2/TC7 cells treated for 24 hours with different concentrations of lactoferrin, whey or buttermilk. None of the substances analyzed caused oxidative damage; however, whey significantly decreased the levels of lipid peroxidation. Furthermore, both lactoferrin and whey were able to reduce the oxidative stress induced by lipopolysaccharide. Respect to TLR receptors, lactoferrin, whey and buttermilk specifically altered the expression of TLR2, TLR4 and TLR9 receptors, with a strong decrease in TLR4 expression. These results suggest that lactoferrin, whey and buttermilk could be interesting potential ingredients for functional foods as they seem to modulate oxidative stress and inflammatory response induced by TLRs activation.

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Proteomic Profiling of the TRAF3 Interactome Network Reveals a New Role for the ER-to-Golgi Transport Compartments in Innate Immunity

PLoS Pathogens ◽

10.1371/journal.ppat.1002747 ◽

2012 ◽

Vol 8 (7) ◽

pp. e1002747 ◽

Cited By ~ 38

Author(s):

Wendy J. van Zuylen ◽

Priscilla Doyon ◽

Jean-François Clément ◽

Kashif Aziz Khan ◽

Lisa M. D'Ambrosio ◽

...

Keyword(s):

Innate Immunity ◽

Proteomic Profiling ◽

Golgi Transport ◽

Interactome Network

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Oxidative stress, innate immunity and sperm quality

Journal of Reproductive Immunology ◽

10.1016/j.jri.2011.06.021 ◽

2011 ◽

Vol 90 (2) ◽

pp. 140

Author(s):

M. Kurpisz ◽

F. Monika ◽

P. Małgorzata ◽

J. Piotr

Keyword(s):

Oxidative Stress ◽

Innate Immunity ◽

Sperm Quality

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Carvacrol, Thymol, and Garlic Essential Oil Promote Skin Innate Immunity in Gilthead Seabream (Sparus aurata) Through the Multifactorial Modulation of the Secretory Pathway and Enhancement of Mucus Protective Capacity

Frontiers in Immunology ◽

10.3389/fimmu.2021.633621 ◽

2021 ◽

Vol 12 ◽

Author(s):

Joana P. Firmino ◽

Laura Fernández-Alacid ◽

Eva Vallejos-Vidal ◽

Ricardo Salomón ◽

Ignasi Sanahuja ◽

...

Keyword(s):

Oxidative Stress ◽

Immune Response ◽

Innate Immunity ◽

Pathogenic Bacteria ◽

Secretory Pathway ◽

Sparus Aurata ◽

Gilthead Seabream ◽

Protective Capacity ◽

Skin Mucus ◽

Garlic Essential Oil

One of the main targets for the use of phytogenics in aquafeeds is the mucosal tissues as they constitute a physical and biochemical shield against environmental and pathogenic threats, comprising elements from both the innate and acquired immunity. In the present study, the modulation of the skin transcriptional immune response, the bacterial growth capacity in skin mucus, and the overall health condition of gilthead seabream (Sparus aurata) juveniles fed a dietary supplementation of garlic essential oil, carvacrol, and thymol were assessed. The enrichment analysis of the skin transcriptional profile of fish fed the phytogenic-supplemented diet revealed the regulation of genes associated to cellular components involved in the secretory pathway, suggesting the stimulation, and recruitment of phagocytic cells. Genes recognized by their involvement in non-specific immune response were also identified in the analysis. The promotion of the secretion of non-specific immune molecules into the skin mucus was proposed to be involved in the in vitro decreased growth capacity of pathogenic bacteria in the mucus of fish fed the phytogenic-supplemented diet. Although the mucus antioxidant capacity was not affected by the phytogenics supplementation, the regulation of genes coding for oxidative stress enzymes suggested the reduction of the skin oxidative stress. Additionally, the decreased levels of cortisol in mucus indicated a reduction in the fish allostatic load due to the properties of the tested additive. Altogether, the dietary garlic, carvacrol, and thymol appear to promote the gilthead seabream skin innate immunity and the mucus protective capacity, decreasing its susceptibility to be colonized by pathogenic bacteria.

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