Dietary supplementation of Pediococcus pentosaceus enhances innate immunity, physiological health and resistance to Vibrio anguillarum in orange-spotted grouper (Epinephelus coioides)

Salmonella enteritidis can cause significant morbidity and mortality in humans and economic loss in the animal industry. Improving the innate immunity is an effective method to prevent S. enteritidis infection. Pediococcus pentosaceus is a Gram-positive coccus which had probiotics properties. Numerous previously published studies reported that probiotics were beneficial to gut microbiota by changing the intestinal flora structure and inhibiting the harmful microbial growth to enhance the innate immunity. We investigated the immunological effects of P. pentosaceus on Salmonella-infected chickens by the following experiment. A total of 120 broilers from AA line were fed and divided into 2 groups (treated and control groups) for the experiment from day 1. The control group was fed with the basic diet, while the treated group was fed with the basic diet adding P. pentosaceus microcapsule with the bacterial concentration of 1 g/kg in the feed and bacterial counts 2.5 × 10 9 CFU/g. All the birds were given with 0.5 ml of S. enteritidis bacterial suspension (109 CFU/ml) through oral cavity at day 9. The number of dead birds was recorded and used in the analysis. The bacterial culture method and quantitative real-time PCR analysis were used to evaluate the effects of P. pentosaceus on chickens infected with S. enteritidis and to ascertain the mechanism of the effect. The results showed that the P. pentosaceus could restrain the pathogenicity of S. enteritidis and reduce the death rate from 44.4% to 23.3%. The flora in the caecum exhibited “rising-declining” trends, and the gene (TLR4, MyD88, TRAF6 NF-κB, IFN-β, TNF-a, IL6, and IL8) expression pattern was different between the experimental and control group. P. pentosaceus as a probiotic may competitively inhibit the growth of S. enteritidis and control the inflammatory response through regulating the gene expression which involved in the toll-like receptor pathway and inflammation pathway.

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Effects of Dietary Supplementation of Barodon, an Anionic Alkali Mineral Complex, on Growth Performance, Feed Utilization, Innate Immunity, Goblet Cell and Digestibility in Olive Flounder (<italic>Paralichthys olivaceus</italic>)

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.2013.13485 ◽

2014 ◽

Vol 27 (3) ◽

pp. 383-390 ◽

Cited By ~ 3

Author(s):

Chang-Hoon Shin ◽

Ji-Hoon Cha ◽

Samad Rahimnejad ◽

Joon-Bum Jeong ◽

Byung-Woo Yoo ◽

...

Keyword(s):

Innate Immunity ◽

Growth Performance ◽

Goblet Cell ◽

Paralichthys Olivaceus ◽

Dietary Supplementation ◽

Feed Utilization ◽

Olive Flounder ◽

Mineral Complex

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Effect of dietary supplementation with Bacillus amyloliquefaciens in the innate immunity in the European sea bass (Dicentrarchus labrax)

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2016.03.079 ◽

2016 ◽

Vol 53 ◽

pp. 70 ◽

Cited By ~ 2

Author(s):

F. Acosta ◽

J. Ramos-Vivas ◽

M. Lazaro-Diez ◽

A. Gonzalez-de Canales ◽

B. Vega ◽

...

Keyword(s):

Innate Immunity ◽

Bacillus Amyloliquefaciens ◽

Dicentrarchus Labrax ◽

Dietary Supplementation ◽

Sea Bass ◽

European Sea Bass

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Autochthonous Probiotics Alleviate the Adverse Effects of Dietary Histamine in Juvenile Grouper (Epinephelus coioides)

Frontiers in Microbiology ◽

10.3389/fmicb.2021.792718 ◽

2021 ◽

Vol 12 ◽

Author(s):

Zi-Yan Liu ◽

Hong-Ling Yang ◽

Ling-Hao Hu ◽

Wei Yang ◽

Chun-Xiang Ai ◽

...

Keyword(s):

Innate Immunity ◽

Adverse Effects ◽

Antioxidant Capacity ◽

Growth Performance ◽

High Dose ◽

Epinephelus Coioides ◽

Gut Health ◽

Microbial Composition ◽

Fatty Acid Binding ◽

Probiotic Strains

High dose (0.3%) of dietary histamine can cause adverse effects on growth performance, innate immunity, and gut health in juvenile grouper (Epinephelus coioides). In the present study, three autochthonous probiotics (Bacillus pumilus SE5, Psychrobacter sp. SE6, and Bacillus clausii DE5) were supplemented separately to diets containing 0.3% of histamine and their effects on growth performance, innate immunity, and gut health of grouper (E. coioides) were evaluated in a 56-day feeding trial. The results showed considerable increase in weight gain, specific growth rate, hepatosomatic index, and decreased feed conversion rate in groupers fed with probiotic-supplemented diets. Supplementation of autochthonous probiotics has improved antioxidant capacity and innate immunity of E. coioides by measuring correlative parameters, such as total antioxidant capacity, superoxide dismutase activity, malondialdehyde content, and so on. Additionally, dietary probiotics have significantly reduced the levels of serum interleukin-1β (at days 28 and 56), fatty acid-binding protein 2, and intestinal trefoil factor (at day 28), and promoted intestinal integrity following remarkably increased muscle thickness and mucosal fold height at day 56, especially in grouper fed with B. pumilus SE5 containing diet (P < 0.05). On day 56, the gut microbial composition of E. coioides was positively shaped by autochthonous probiotics, the relative abundance of potentially pathogenic Photobacterium decreased while beneficial Lactobacillus increased in fish fed with probiotic strains, especially with B. pumilus SE5 and B. clausii DE5. These results suggest that among the three autochthonous probiotic strains tested, B. pumilus SE5 is showing better efficiency in alleviating the adverse effects of (high levels) dietary histamine by decreasing the expression of inflammatory markers and by improving the growth, innate immunity, and gut health of juvenile grouper E. coioides.

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IFP35 Is a Relevant Factor in Innate Immunity, Multiple Sclerosis, and Other Chronic Inflammatory Diseases: A Review

Biology ◽

10.3390/biology10121325 ◽

2021 ◽

Vol 10 (12) ◽

pp. 1325

Author(s):

Roberto De Masi ◽

Stefania Orlando ◽

Francesco Bagordo ◽

Tiziana Grassi

Keyword(s):

Multiple Sclerosis ◽

Innate Immunity ◽

Leucine Zipper ◽

Nuclear Translocation ◽

Response To Therapy ◽

Demyelinating Diseases ◽

In Vivo Studies ◽

Type I ◽

Epinephelus Coioides ◽

Human Pathology

Discovered in 1993 by Bange et al., the 35-kDa interferon-induced protein (IFP35) is a highly conserved cytosolic interferon-induced leucine zipper protein with a 17q12-21 coding gene and unknown function. Belonging to interferon stimulated genes (ISG), the IFP35 reflects the type I interferon (IFN) activity induced through the JAK-STAT phosphorylation, and it can homodimerize with N-myc-interactor (NMI) and basic leucine zipper transcription factor (BATF), resulting in nuclear translocation and a functional expression. Casein kinase 2-interacting protein-1 (CKIP-1), retinoic acid-inducible gene I (RIG-I), and laboratory of genetics and physiology 2 Epinephelus coioides (EcLGP2) are thought to regulate IFP35, via the innate immunity pathway. Several in vitro and in vivo studies on fish and mammals have confirmed the IFP35 as an ISG factor with antiviral and antiproliferative functions. However, in a mice model of sepsis, IFP35 was found working as a damage associated molecular pattern (DAMP) molecule, which enhances inflammation by acting in the innate immune-mediated way. In human pathology, the IFP35 expression level predicts disease outcome and response to therapy in Multiple Sclerosis (MS), reflecting IFN activity. Specifically, IFP35 was upregulated in Lupus Nephritis (LN), Rheumatoid Arthritis (RA), and untreated MS. However, it normalized in the MS patients undergoing therapy. The considered data indicate IFP35 as a pleiotropic factor, suggesting it as biologically relevant in the innate immunity, general pathology, and human demyelinating diseases of the central nervous system.

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