Evaluation of change in antibiotic sensitivity pattern of E. coli isolates from urinary tract infections in a tertiary care hospital

2015 ◽  
Vol 3 (2) ◽  
pp. 73
Author(s):  
Mistry Yogita ◽  
Rajdev Sangita ◽  
Mulla Summaiya
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Care Hospital ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Tract Infections

scholarly journals Antibiotic sensitivity pattern of pathogens in children with urinary tract infection in a tertiary care hospital in Kachchh, Gujarat, India

2017 ◽  
Vol 4 (6) ◽  
pp. 2103
Author(s):  
Rekha Thaddanee ◽  
Gurudas Khilnani ◽  
Nupur Shah ◽  
Ajeet Kumar Khilnani
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Care Hospital ◽  
Coagulase Negative Staphylococci ◽  
E Coli ◽  
Sensitivity Pattern

Background: This prospective observational study was conducted in a tertiary care hospital in Kachchh, over a period of 6 months, to know the antibiotic sensitivity pattern of pathogens in children less than 18 years old with Urinary Tract Infection (UTI).Methods: Between December 2016 to June 2017, 186 children met the inclusion criterion. Urine samples were collected and processed for urine routine microscopy, culture and antibiotic sensitivity as per the standard laboratory guidelines. Urine culture was positive in 50 cases, which were further evaluated to find out any renal disease. All patients were given 10-14 days antibiotics course and follow-up urine reports were done. Patients were considered cured when the follow-up urine reports were normal.Results: E. coli was the commonest organism (34%) isolated, found mainly in 1-5 years age group (20%). Enterococci were isolated in 32% cases, most of which were less than 5 years of age (28%). Klebsiella (12%), Methicillin Resistant Staphylococcus Aureus (MRSA) (10%), Coagulase negative staphylococci (6%), Pseudomonas (2%) and Budding yeast cell (4%) were the less frequent organisms isolated. E. coli were found to be less sensitive to different Aminoglycosides (11.7% - 23.5%), Cephalosporins (11.7% - 52.9%), Fluoroquinolones (5.8% - 11.7%), Co-trimoxazole (17.6%) and Piperacillin (17.6%), but were more sensitive (70.6%) to Imipenems. Enterococci were also partially sensitive to Aminoglycosides (6.2-18.7%), Cephalosporins (25-37.5.7%), Fluoroquinolones (6.25-12.5%), Penicillin-G (50%), Piperacillin (31.25%) and Co-trimoxazole (43.75%), but had good sensitivity for Imipenems (68.7%), Linezolid (75%) and Vancomycin (81.2%). Similarly, MRSA was 100% sensitive to Linezolid and Imipenem but partially sensitive (20-60%) to other antibiotics. Klebsiella showed 16.65-33.3% sensitivity to all antibiotics except Imipenem (83.3%) and was 100% resistant to Co-trimoxazole. Coagulase negative Staphylococci (CONS) remained 100% sensitive to all antibiotics and Pseudomonas was resistant to all antibiotics.Conclusions: The study concludes that pathogens for UTI in children have developed resistance, even to the newer generation antibiotics, probably due to the irrational use of antibiotics. In view of emergence of multi drug resistant pathogens, which carry considerable morbidity and mortality, every effort must be taken to use antibiotics judiciously.

Determination of ESBL Production & Antibiotic Resistance Profile of Escherichia Coli Isolated from Patients with Urinary Tract Infections: A Study from Tertiary Care Hospital

2020 ◽  
Vol 7 (1) ◽  
pp. 23-29
Author(s):  
Gunjal P. N. ◽  
Gunjal S. P.
Keyword(s):  
Urinary Tract ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Multidrug Resistant ◽  
Care Hospital ◽  
Esbl Production ◽  
Sensitivity Testing ◽  
E Coli ◽  
Tract Infections

Urinary tract infection (UTI), is defined as a disease caused by invasion of urinary tract by microorganisms. Majority of UTI cases are due to bacterial infection constitute about 95% of total UTI cases. About 80% of UTI cases are caused by E.coli producing extended spectrum ?-lactamase (ESBL) producing isolates. In recent years limitations in treating infections caused by multidrug resistant organisms has increased. This study aims to determine ESBL production of E. coli cases from a tertiary care hospital. Methodology: A total 358 midstream urine samples were collected by random sampling method during March 2015 to June 2018. Identification, antibiotic sensitivity testing, performed according to standard protocol following Clinical and Laboratory Standard Institute (CLSI) guidelines, 2013. Screening for ESBL producing E.coli isolates performed using ceftazidime further confirmation done by phenotypic disc diffusion test using combined disc method using ceftazidime (30µg) & ceftazidime/ clavulanic acid (30/10 µg) as per CLSI guidelines. Results: Total 358 specimens processed for urine culture. Gram negative bacilli isolated from 123(34.35 %), out of which 68 (55.28%) were E.coli, 19 (15.44%) K. pneumoniae, 15 (12.19%), Pseudomonas spp. 08 (6.50%), Citrobacter spp and Acinetobacter spp, 03 (2.43%), Proteus mirabilis, 01 (0.81%) Proteus vulgaris and Enterobacter respectively. Out of 68 isolates of E.coli, 65 (95.58%) were MDR, ESBL was detected in 31 (47.69%) out of these 65 isolates. Out of these 31 cases 19 (61.29%) were female and 12 (38.70%) were male cases. Conclusion: This study concludes 47.69% ESBL producing MDR E. coli were isolated from UTI cases with female predominance.

scholarly journals Microbes and antibiotic susceptibility patterns of urinary tract infections in toilet-trained Children at a Tertiary Care Hospital of Sialkot, Pakistan.

2021 ◽  
Vol 28 (01) ◽  
pp. 22-26
Author(s):  
Syed Muhammad Hassan Akhtar ◽  
Abdul Sattar ◽  
Wajiha Rizwan ◽  
Naeem Ahmed Cheema ◽  
Aftab Anwar
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Rural Areas ◽  
Urine Analysis ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Care Hospital ◽  
Amoxicillin Clavulanate ◽  
Tract Infections

Objective: To find out various types of microorganisms causing urinary tract infections and their antibiotic sensitivity patterns among toilet-trained children presenting at a tertiary care hospital of Sialkot District. Study Design: Descriptive study. Setting: Department of Microbiology/Urology, Khawaja Muhammad Safdar Medical College, Sialkot. Period: June to December 2019. Material & Methods: A total of 176 toilet trained children, aged 7 to 15 years, presenting in outpatient department and having culture positive urinary tract infections were enrolled. Demographic information like age, gender and area of residence along with clinical findings, antibiotic sensitivities and resistance patterns were recorded. Mid-stream urine sample was collected from all toilet-trained children and immediately sent to institutional laboratory for urine analysis and urine culture and sensitivities. Results: Out of a total of 176 children, 95 (54.0%) were female and 81 (46.0%) male. Overall mean age was noted to be 10.68+2.42 years while most of the children, 94 (53.4%) were above 10 years of age. Majority of the patients, 98 (55.7%) belonged to rural areas. Eschericia coli were the most common isolate, found among 140 (79.5%) children. Imipenem (98.9%), meropenem (98.9%), piperacillin tazobactam (97.7%), fosfomycin (96.6%), amikacin (95.5%), nitrofurantoin (84.4%), gentamycin (90.9%) and amoxicillin clavulanate (83%) were found to have the highest sensitivities. Conclusion: Eschericia coli were the most common bacterial urinary pathogens in toilet-trained children. Amoxicillin clavulanate, Nitrofurantoin and Fosfomycin available in oral form showed good sensitivity of 83%, 84.4%, and 96.6% respectively) to commonly found microorganisms. It was also noted that resistance to commonly used antibiotics in our region is rising.

scholarly journals Prevalence of urinary tract infection and sensitivity pattern amongst children less than 3 years of age with fever in a tertiary care hospital in South Karnataka

2020 ◽  
Vol 9 (5) ◽  
pp. 736
Author(s):  
Jasmine John Ponvelil ◽  
Hema Narasimhe Gowda ◽  
Savitha Mysore Ram Raj
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Tertiary Care ◽  
Colony Count ◽  
Resistance Pattern ◽  
Care Hospital ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Tract Infections

Background: Antimicrobial resistance is a major concern especially in urinary tract infections in children as improperly treated urinary tract infections (UTIs) on long term can cause renal scarring in young children, which leads to long term morbidities like hypertension, chronic renal disease and pre-eclampsia. The empirical therapy for UTI varies regionally due to their varied sensitivities and resistance pattern. This study aims to facilitate policy making in empirical antibiotic therapy of pediatric patients with urinary tract infections.Methods: A cross sectional study, which included a group of 140 children’s (6 months to 3 years) with fever were included in the study. All patients with colony count >1 lakh CFU/ml or colony count >50,000 CFU/ml, with leukocyturia (>5 WBCs/HPF in centrifuged urine) or colony count >1000 CFU/ml with urinary symptoms were diagnosed to be positive for urinary tract infection.Results: The prevalence of UTI in febrile children less than 3 years in our study was 0.1%. Out of 140 patients with fever, 35 were UTI positive. E. coli was the most commonly isolated organism (60%), followed by MRSA (14.2%) and Enterococci (11.4 %). E. coli was found to be most sensitive to nitrofurantoin (85.7%), followed by gentamicin (61.9%) and norfloxacin (38%). E. coli showed high resistance to cefuroxime (76.2%) and ceftriaxone (71.4%). MRSA was found to be most sensitive to linezolid and vancomycin, and resistant to norfloxacin.Conclusions: The data shows the increased resistance of E. coli to commonly prescribed antibiotics like cefuroxime and ceftriaxone. So, this study shows the empirical treatment of UTI in our region and the importance of having antibiotic prescription policies in every region.

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