Physical interactions of HLA-G with MHC class I partner molecules on the surface of two trophoblast cell lines, JEG-3 and ACH-3P, by Förster resonance energy transfer based on fluorescence lifetime measurements

2010 ◽  
Vol 86 (2) ◽  
pp. 90-91
Author(s):  
A. Jabeen ◽  
P. Jain ◽  
P.P. Laissue ◽  
K. Suhling ◽  
J.A. Levitt ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Mhc Class I ◽  
Cell Lines ◽  
Fluorescence Lifetime ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Trophoblast Cell ◽  
Class I ◽  
Lifetime Measurements ◽  
Physical Interactions

Automated multiwell fluorescence lifetime imaging for Förster resonance energy transfer assays and high content analysis

2015 ◽  
Vol 7 (10) ◽  
pp. 4071-4089 ◽  
Author(s):  
Douglas J. Kelly ◽  
Sean C. Warren ◽  
Dominic Alibhai ◽  
Sunil Kumar ◽  
Yuriy Alexandrov ◽  
...  
Keyword(s):  
Content Analysis ◽  
Energy Transfer ◽  
Fluorescence Lifetime ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Förster Resonance Energy Transfer ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
High Content Analysis ◽  
Förster Resonance

An HCA-FLIM instrument is presented alongside exemplar oligomerisation, intermolecular and intramolecular FRET assays that require robust measurement of small lifetime changes.

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