Selection of macroinvertebrate metrics for rapid assessment of the human impact by biotic conditions of Bolivian Altiplano streams

Author(s):  
Carlos I. Molina ◽  
M. Julio Pinto ◽  
C. Dario Achá
Author(s):  
Steven L. McMurtry ◽  
Susan J. Rose ◽  
Lisa K. Berger

Accurate measurement is essential for effective social work practice, but doing it well can be difficult. One solution is to use rapid assessment instruments (RAIs), which are brief scales that typically require less than 15 minutes to complete. Some are administered by practitioners, but most are self-administered on paper or electronically. RAIs are available for screening, initial assessment, monitoring of service progress, and outcome evaluation. Some require author permission, others are sold commercially, and many more are free and in the public domain. Selection of an RAI should be based first on its psychometric strength, including content, concurrent, and known-groups validity, as well as on types of reliability such as internal consistency, but practical criteria such as readability are also important. And when used in practice settings, RAIs should be part of a well-rounded measurement plan that also includes behavioral observations, client logs, unobtrusive measures, and other approaches.


2017 ◽  
Vol 34 (4) ◽  
pp. 371-386 ◽  
Author(s):  
Eduardo Coutinho ◽  
Klaus R. Scherer

The systematic study of music-induced emotions requires standardized measurement instruments to reliably assess the nature of affective reactions to music, which tend to go beyond garden-variety basic emotions. We describe the development and conceptual validation of a checklist for rapid assessment of music-induced affect, designed to extend and complement the Geneva Emotional Music Scale. The checklist contains a selection of affect and emotion categories that are frequently used in the literature to refer to emotional reactions to music. The development of the checklist focused on an empirical investigation of the semantic structure of the relevant terms, combined with fuzzy classes based on a series of hierarchical cluster analyses. Two versions of the checklist for assessing the intensity and frequency of affective responses to music are proposed.


2021 ◽  
Author(s):  
Rahul Mahadev Shelake ◽  
Dibyajyoti Pramanik ◽  
Jae-Yean Kim

Rapid assessment of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based tools and validation of cloned CRISPR-plasmid vectors are critical aspects for the successful application of genome editing (GE) in different organisms, including plants. Also, the selection of active guide RNAs (gRNAs) is a determining factor to achieve efficient GE at the targeted locus. Incidentally, plasmid vectors capable of ectopic expression of Cas enzyme and sgRNAs in bacteria will facilitate the quick screening and reliability of cloned plasmids and the functionality of designed gRNAs. We report a platform for in vivo rapid investigation of CRISPR components in Escherichia coli (IRI-CCE), which is compatible with plant-transforming binary vectors comprising plant promoters/terminators. Besides, IRI-CCE analyses reveal distinct features of cytidine (PmCDA1, evoCDA1, APOBEC3A) and adenine (ABE8e) base editors. Finally, we show the IRI-CCE platform as a reliable and rapid method for screening functional gRNAs to achieve successful GE outcomes. Base editor-based CRISPR components expressed by promoters of different strengths led to the establishment of IRI-CCE platform for three major applications: optimization of novel CRISPR tools, validation of cloned CRISPR plasmids, and to know gRNA functionality.


2014 ◽  
Vol 1036 ◽  
pp. 800-805
Author(s):  
Mariusz Piotr Hetmańczyk ◽  
Piotr Michalski

Development of devices for the automatic control of the state of the elements that are components of cars has been presented in this article. In spite of the diversity-controlled parts authors have identified the essential elements of each of the positions. Each unit is composed of a housing base plate - with a notch adapted to the controlled element skeleton - part responsible for the determination of controlled parts [, electronic control system detail, visual signaling allowing for rapid assessment of the inspection. A noticeable fact is related to the necessity of proper selection of industrial sensor technology for specific applications. The premises, existence of which can cancel the interop control in favor of the final inspection was been shown by the authors. Considering the most popular industrial sensor systems, with respect to the following criteria measuring range, resolution, sensitivity, also an important factor in the selection [ of the actuators and control subsystem has been discussed. Also the general algorithm of the automatic process control with the methodology of Poka-Yoke has been presented.


2012 ◽  
Vol 22 ◽  
pp. 587-596 ◽  
Author(s):  
Marcelo M. Azambuja ◽  
William J. O'Brien

Zootaxa ◽  
2018 ◽  
Vol 4466 (1) ◽  
pp. 205 ◽  
Author(s):  
MARIE GRENIER ◽  
CÉSAR RUIZ ◽  
MAIA FOURT ◽  
MATHIEU SANTONJA ◽  
MAUDE DUBOIS ◽  
...  

Mediterranean sponges represent about 10 % of the world sponge biodiversity, with these sessile organisms dominating in terms of diversity and biomass in most of the rocky bottoms shaded from light. After 60 years of intensive study of the sponge diversity along the French coast, we present the first comprehensive reference-list for this biogeographic area. A total of 389 sponge species was recorded, of which 222 known in the Marseille region. In this area, special attention was paid to species from underwater caves. Although this particular habitat appeared as one of the richest, a wealth of hidden diversity still requires description. About 37 % of the sponge diversity can be found in underwater caves, most of these species being also distributed in other habitats. However, 23 % of this sponge diversity is cave-exclusive. An easy and rapid assessment method was developed with a selection of 65 representative sponge species, for the monitoring of semi-dark cave communities. This method, based on data acquisition with photoquadrats and their processing using a DataBase built with ACCESS, was deployed in 13 studied sites. Altogether, this study represents a useful contribution for marine environment managers who might refer to this French reference list and apply the rapid and easy assessment method in the framework of several European Directives and international Conventions. 


2011 ◽  
Vol 64 (4) ◽  
pp. 999-1008 ◽  
Author(s):  
M. A. Dennis ◽  
M. Landman ◽  
S. A. Wood ◽  
D. Hamilton

Flow cytometry has potential as a rapid assessment technique to evaluate phytoplankton biomass and species composition. It facilitates for multi-parameter analysis of individual cells on the basis of light scattering effects induced from cellular constituents, as well as auto-fluorescence. Fluorescence emission characteristics may be especially useful in classifying cyanobacteria as they contain phycoerythrin which emits light predominantly in the 550–600 nm waveband, chlorophyll-a (650–700 nm emission) and allophycocyanin (660 nm emission). The objective of our study was to assess the utility of flow cytometry for the rapid identification and sorting of freshwater algae and cyanobacteria species. Using a selection of laboratory-cultured freshwater algae and cyanobacteria species, this study demonstrated unique light scatter and fluorescent characteristics for each species examined, allowing for rapid species identification and sorting of mixed populations of laboratory cultures and samples from two lakes in the Rotorua region (New Zealand). Analysis of lake water samples collected over seven months demonstrated changes in abundance and community composition of phytoplankton in the two lakes and demonstrates that flow cytometry may be a useful technique for examining seasonal changes in phytoplankton composition.


2018 ◽  
Author(s):  
L.M. Sokolova

Во многих регионах РФ и странах ближнего зарубежья отмечено усиление вредоносности болезней моркови, вызванных грибами рр. Fusarium и Alternaria. В зависимости от погодных условий и фитосанитарного состояния посевов распространённость болезней может достигать 70 – 80 %, а урожайность корнеплодов снижается на 35-50 %. Одним из путей, обеспечивающих целенаправленное ведение селекции на устойчивость, является выделение местных изолятов возбудителей болезней, методы ускоренной оценки на основе определения агрессивности новых штаммов и применение их в селекционной работе. Цель работы: выделить местные изоляты возбудителей болезней и определить их агрессивность. Отбор пораженного материала для исследований проводили на опытных делянках ФГБНУ ВНИИО (Московская обл.). Опыты по определению агрессивности выделенных штаммов возбудителей рр. Alternaria и Fusarium проводили с 2007 по 2014 гг. лабораторным методом. В результате проделанной работы создана коллекция наиболее агрессивных местных штаммов патогенов: Fusarium avenaceum Sacc (Т7 - F1), Fusarium oxysporum Schlecht (ПО3 – F3), и Ш3, Alternaria radicina M., Dr. Et E - ВС-1-1А; ВС-2-1А; М – 2 – 3А; А-1 и А-3. Приведено описание мицелия по штамму р. Fusarium: Ш3 – штамм формирует пушистую бело-розовую колонию и обильное спороношение. Рассмотрена градация варьирования штаммов р. Fusarium - от белых войлочных и слизистых до бело - розовых пушистых. Выделенные наиболее агрессивные штаммы используются в селекционной работе над повышением устойчивости моркови столовой при проведении опытов по опрыскиванию суспензией спор по листовой пластине моркови столовой и при создании искусственных инфекционных фонов. Продолжается работа по выделению, идентификации и определению агрессивности возбудителей альтернариоза и фузариоза столовой моркови, а также по подбору образцов моркови столовой - контролей устойчивости к болезням.In many regions of the Russian Federation and the countries of the near abroad, there was an increase in the severity of carrot diseases caused by fungi of. Fusarium and Alternaria. Depending on the weather conditions and the phytosanitary condition of crops, the prevalence of diseases can reach 70-80%, and the yield of root crops is reduced by 35-50%. One of the ways ensuring targeted selection of sustainability is the isolation of local isolates of pathogens, rapid assessment methods based on the aggressiveness of new strains and their use in breeding. Objective: to isolate local isolates of pathogens and determine their aggressiveness. Selection of the affected material for the studies was carried out on experimental plots of the FGBNU VNIIO (Moscow region). Experiments to determine the aggressiveness of isolated strains of pathogens Alternaria and Fusarium were conducted from 2007 to 2014 years of laboratory method. As a result of the work done, a collection of the most aggressive local strains of pathogens was created: Fusarium avenaceum Sacc (T7 - F1), Fusarium oxysporum Schlecht (PO3 - F3), and III, Alternaria radicina M., Dr. Et E - ВС-1-1А; ВС-2-1А; M - 2 - 3A; A-1 and A-3. The description of the mycelium on the strain of Fusarium is given: III - the strain forms a fluffy white-pink colony and abundant sporulation. The gradation of variation in the strains of Fusarium - from white felt and mucous to white - pink fluffy. Highlighted most aggressive strains are used in breeding work to increase the stability of carrots canteen when conducting experiments on spraying spore suspension on a plate of carrot dining room and when creating artificial infectious backgrounds. Work continues on the identification, identification and determination of the aggressiveness of pathogens of Alternaria and Fusarium of carrots, as well as the selection of carrot samples - disease resistance control.


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