Выделение и агрессивность возбудителей болезней родов Fusarium и Аlternaria на моркови столовой

2018 ◽  
Author(s):  
L.M. Sokolova

Во многих регионах РФ и странах ближнего зарубежья отмечено усиление вредоносности болезней моркови, вызванных грибами рр. Fusarium и Alternaria. В зависимости от погодных условий и фитосанитарного состояния посевов распространённость болезней может достигать 70 – 80 %, а урожайность корнеплодов снижается на 35-50 %. Одним из путей, обеспечивающих целенаправленное ведение селекции на устойчивость, является выделение местных изолятов возбудителей болезней, методы ускоренной оценки на основе определения агрессивности новых штаммов и применение их в селекционной работе. Цель работы: выделить местные изоляты возбудителей болезней и определить их агрессивность. Отбор пораженного материала для исследований проводили на опытных делянках ФГБНУ ВНИИО (Московская обл.). Опыты по определению агрессивности выделенных штаммов возбудителей рр. Alternaria и Fusarium проводили с 2007 по 2014 гг. лабораторным методом. В результате проделанной работы создана коллекция наиболее агрессивных местных штаммов патогенов: Fusarium avenaceum Sacc (Т7 - F1), Fusarium oxysporum Schlecht (ПО3 – F3), и Ш3, Alternaria radicina M., Dr. Et E - ВС-1-1А; ВС-2-1А; М – 2 – 3А; А-1 и А-3. Приведено описание мицелия по штамму р. Fusarium: Ш3 – штамм формирует пушистую бело-розовую колонию и обильное спороношение. Рассмотрена градация варьирования штаммов р. Fusarium - от белых войлочных и слизистых до бело - розовых пушистых. Выделенные наиболее агрессивные штаммы используются в селекционной работе над повышением устойчивости моркови столовой при проведении опытов по опрыскиванию суспензией спор по листовой пластине моркови столовой и при создании искусственных инфекционных фонов. Продолжается работа по выделению, идентификации и определению агрессивности возбудителей альтернариоза и фузариоза столовой моркови, а также по подбору образцов моркови столовой - контролей устойчивости к болезням.In many regions of the Russian Federation and the countries of the near abroad, there was an increase in the severity of carrot diseases caused by fungi of. Fusarium and Alternaria. Depending on the weather conditions and the phytosanitary condition of crops, the prevalence of diseases can reach 70-80%, and the yield of root crops is reduced by 35-50%. One of the ways ensuring targeted selection of sustainability is the isolation of local isolates of pathogens, rapid assessment methods based on the aggressiveness of new strains and their use in breeding. Objective: to isolate local isolates of pathogens and determine their aggressiveness. Selection of the affected material for the studies was carried out on experimental plots of the FGBNU VNIIO (Moscow region). Experiments to determine the aggressiveness of isolated strains of pathogens Alternaria and Fusarium were conducted from 2007 to 2014 years of laboratory method. As a result of the work done, a collection of the most aggressive local strains of pathogens was created: Fusarium avenaceum Sacc (T7 - F1), Fusarium oxysporum Schlecht (PO3 - F3), and III, Alternaria radicina M., Dr. Et E - ВС-1-1А; ВС-2-1А; M - 2 - 3A; A-1 and A-3. The description of the mycelium on the strain of Fusarium is given: III - the strain forms a fluffy white-pink colony and abundant sporulation. The gradation of variation in the strains of Fusarium - from white felt and mucous to white - pink fluffy. Highlighted most aggressive strains are used in breeding work to increase the stability of carrots canteen when conducting experiments on spraying spore suspension on a plate of carrot dining room and when creating artificial infectious backgrounds. Work continues on the identification, identification and determination of the aggressiveness of pathogens of Alternaria and Fusarium of carrots, as well as the selection of carrot samples - disease resistance control.

2021 ◽  
Vol 244 ◽  
pp. 11055
Author(s):  
Marianna Gladkikh ◽  
Olga Kuznetsova

Race speed is one of the main indicators by which trotting horses are estimated. In addition to the horse genotype, race speed is also determined by the influence of environmental factors, such as feeding, keeping, training, and testing conditions. These conditions include of race track surface condition, the rider’s qualifications, season of the year, weather conditions. In this paper the influence of race track surface condition on the accuracy of assessing race time is considered. The data of horse trials of Orlov trotters at the Moscow hippodrome were used. The race time of 256 horses at a distance of 1600 m was assessed. Depending on the weather conditions and the track surface, 9 different track conditions were identified. For the analysis we used data on stallions and mares aged 4 years and older without disaggregation by sex, since there were no significant differences between the average race speed of stallions and mares and it was shown that it was the horses of the older age group that were reliably characterized by the highest average race time with minimal races per horse. It was found that the strength of the influence of the variety of race tracks on the variety of assessments of race time is reliable and amounted to 23.5%. When analyzing the group of the fastest horses, this indicator turned out to be higher and amounts to 43.8%. It is also shown that the selection of horses for breeding work is more expedient and accurate to carry out according to the average race time of the horse for a number of races, and not according to its best result, since in this case the stability of performances is not taken into account.


2019 ◽  
pp. 1-23
Author(s):  
А.В. Семенютина ◽  
С.Е. Лазарев ◽  
К.А. Мельник

Представители родовых комплексов Robinia (Робиния), Gleditsia (Гледичия) относятся к экономически важным и перспективным, для деградированных ландшафтов засушливого региона, видам растений. Несмотря на это до последнего времени отсутствуют сведения по многим видам этих родовых комплексов, связанные с вопросами экологических основ семеноведения и их репродуктивной способности в условиях светлокаштановых почв Волгоградской области. Все это направлено на выявление и расширение перспективных ареалов культивирования для лесомелиорации и озеленения населенных пунктов. Цель исследований изучение репродуктивной способности представителей родовых комплексов Robinia (Робиния), Gleditsia (Гледичия) в коллекциях ФНЦ агроэкологии РАН и выявление особенностей их селекционного семеноведения для лесомелиорации и озеленения населенных пунктов в сухостепных условиях. Объектами исследований являлись виды и формы рода Robinia: R. viscosa Vent. R. neomexicana Gray. R. pseudoacacia L. R. neomexicana х pseudoacacia, и Gleditsia (G. triacanthos L., G. triacanthos L. f. inermis, G. caspica Desf, G. texana Sarg., G. aquatica, G. japonica, G. caspica, G. sinensis), произрастающие в кластерных участках коллекций ФНЦ агроэкологии РАН, кадастр 34:34:000000:122, 34:34:060061:10. Разработка основ селекционного семеноведения базируется на изучении репродуктивных особенностей выделенных для целевого использования собственных биоресурсов с учетом возможностей всестороннего изучения генеративных качеств и оценки биологического потенциала. Выявлено, что стабильность плодоношения фиксируется через несколько лет после вступления в генеративную фазу: у Robinia и Gleditsia 67 лет. Первое цветение у Gleditsia triacanthos и G. texana, G. aquatica наблюдалось в возрасте 5 лет, G. japonica 6, G. caspica и G. sinensis 8 лет. Завязываемость плодов зависит не только от возраста растения, но и от погодных условий во время цветения. На семенную продуктивность влияет количество выпавших осадков и сумма активных температур в период созревания плодов. Формирование более крупных плодов и семян наблюдается в возрасте до 15 лет. В результате исследований (20172019 гг.) выявлено влияние лимитирующих факторов на биологический потенциал цветения, плодоношения и семенную продуктивность для определения ареалов их культивирования. Representatives of generic systems Robinia, Gleditsia are economically important and promising for degraded arid landscapes of the region, types of plants. Despite this, until recently, there is no information on many types of these generic complexes related to the environmental foundations of seed and their reproductive capacity in light chestnut soils of the Volgograd region. All this is aimed at identifying and expanding promising areas of cultivation for forest reclamation and greening of settlements. The aim of the research is to study the reproductive capacity of representatives of ancestral complexes Robinia, Gleditsia in the collections of the Federal scientific center for Agroecology Russian Academy of Sciences and the identifying features of their selection of seed for forest reclamation and landscaping of settlements in the dry steppe conditions. The objects of research were species and forms of the genus Robinia: R. viscosa Vent. R. neomexicana Gray. R. pseudoacacia L. R. neomexicana x pseudoacacia and Gleditsia (G. triacanthos L., G. triacanthos L. f. inermis, G. caspica Desf, G. texana Sarg., G. aquatica, G. japonica, G. caspica, G. sinensis), growing in the cluster areas of collections of FSC Agroecology RAS, cadastre 34:34:000000:122, 34:34:060061:10. The development of the principles of seed breeding is based on the study of reproductive characteristics of the allocated for the targeted use of their own bioresources, taking into account the possibilities of a comprehensive study of generative qualities and assessment of biological potential. It was found that the stability of fruiting is fixed a few years after entering the generative phase: Robinia and Gleditsia 67 years. The first flowering in Gleditsia triacanthos and G. texana, G. aquatica was observed at the age of 5 years, G. japonica 6, G. caspica and G. sinensis 8 years. Fruit setability depends not only on the age of the plant, but also on weather conditions during flowering. Seed productivity is affected by the amount of precipitation and the amount of active temperatures during the ripening period. The formation of larger fruits and seeds is observed in the age of 15 years. As a result of studies (20172019), the influence of limiting factors on the biological potential of flowering, fruiting and seed production to determine the areas of their cultivation was revealed.


Author(s):  
A.A. Dronic A.A. ◽  

The article presents an assessment of the stability of introduced cherry varieties to spring return frosts in 2020 in the conditions of the sharply continental climate of the Astrakhan region. As a result of unfavorable weather conditions, the total damage score of all varieties was 2-5 points. Almost all the studied varieties showed an insufficient level of resistance to recurrent frosts.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuping Li ◽  
Xiaoju Liang ◽  
Xuguo Zhou ◽  
Yu An ◽  
Ming Li ◽  
...  

AbstractGlycyrrhiza, a genus of perennial medicinal herbs, has been traditionally used to treat human diseases, including respiratory disorders. Functional analysis of genes involved in the synthesis, accumulation, and degradation of bioactive compounds in these medicinal plants requires accurate measurement of their expression profiles. Reverse transcription quantitative real-time PCR (RT-qPCR) is a primary tool, which requires stably expressed reference genes to serve as the internal references to normalize the target gene expression. In this study, the stability of 14 candidate reference genes from the two congeneric species G. uralensis and G. inflata, including ACT, CAC, CYP, DNAJ, DREB, EF1, RAN, TIF1, TUB, UBC2, ABCC2, COPS3, CS, R3HDM2, were evaluated across different tissues and throughout various developmental stages. More importantly, we investigated the impact of interactions between tissue and developmental stage on the performance of candidate reference genes. Four algorithms, including geNorm, NormFinder, BestKeeper, and Delta Ct, were used to analyze the expression stability and RefFinder, a comprehensive software, provided the final recommendation. Based on previous research and our preliminary data, we hypothesized that internal references for spatio-temporal gene expression are different from the reference genes suited for individual factors. In G. uralensis, the top three most stable reference genes across different tissues were R3HDM2, CAC and TUB, while CAC, CYP and ABCC2 were most suited for different developmental stages. CAC is the only candidate recommended for both biotic factors, which is reflected in the stability ranking for the spatio (tissue)-temporal (developmental stage) interactions (CAC, R3HDM2 and DNAJ). Similarly, in G. inflata, COPS3, R3HDM2 and DREB were selected for tissues, while RAN, COPS3 and CS were recommended for developmental stages. For the tissue-developmental stage interactions, COPS3, DREB and ABCC2 were the most suited reference genes. In both species, only one of the top three candidates was shared between the individual factors and their interactions, specifically, CAC in G. uralensis and COPS3 in G. inflata, which supports our overarching hypothesis. In summary, spatio-temporal selection of reference genes not only lays the foundation for functional genomics research in Glycyrrhiza, but also facilitates these traditional medicinal herbs to reach/maximize their pharmaceutical potential.


2021 ◽  
Vol 11 (5) ◽  
pp. 2106
Author(s):  
Abdelali El Aroudi ◽  
Mohamed Debbat ◽  
Mohammed Al-Numay ◽  
Abdelmajid Abouloiafa

Numerical simulations reveal that a single-stage differential boost AC module supplied from a PV module under an Maximum Power Point Tracking (MPPT) control at the input DC port and with current synchronization at the AC grid port might exhibit bifurcation phenomena under some weather conditions leading to subharmonic oscillation at the fast-switching scale. This paper will use discrete-time approach to characterize such behavior and to identify the onset of fast-scale instability. Slope compensation is used in the inner current loop to improve the stability of the system. The compensation slope values needed to guarantee stability for the full range of operating duty cycle and leading to an optimal deadbeat response are determined. The validity of the followed procedures is finally validated by a numerical simulations performed on a detailed circuit-level switched model of the AC module.


2021 ◽  
Vol 13 (15) ◽  
pp. 8170
Author(s):  
Veronica Sanda Chedea ◽  
Ana-Maria Drăgulinescu  ◽  
Liliana Lucia Tomoiagă  ◽  
Cristina Bălăceanu ◽  
Maria Lucia Iliescu 

Known for its dry and semi-dry white wine, the Târnave vineyard located in central Transylvania is challenged by the current climate change, which has resulted in an increase of the period of active vegetation by approximately 15–20 days, the average annual temperature by 1–1.5 °C and also the amount of useful temperatures (useful thermal balance for the grapevine). Furthermore, the frost periods have been reduced. Transylvania is an important Romanian region for grapevine cultivation. In this context, one can use the climatic changes to expand their wine assortment by cultivating an autochthonous grapevine variety called Amurg. Amurg is a red grape cultivar homologated at SCDVV Blaj, which also homologated 7 cultivars and 11 clones. Because viticulture depends on the stability of meteorological and hydrological parameters of the growing area, its foundations are challenged by climate change. Grapevine production is a long time investment, taking at least five years before the freshly planted vines produce the desired quality berries. We propose the implementation of a climate change-based precision viticulture turn-key solution for environmental monitoring in the Târnave vineyard. This solution aims to evaluate the grapevine’s micro-climate to extend the sustainable cultivation of the Amurg red grapes cultivar in Transylvania with the final goal of obtaining Protected Designation of Origin (PDO) rosé and red wines from this region. Worldwide, the changing conditions from the existing climate (a 30-year average), used in the past hundred years to dictate local standards, such as new and erratic trends of temperature and humidity regimes, late spring freezes, early fall frosts, storms, heatwaves, droughts, area wildfires, and insect infestations, would create dynamic problems for all farmers to thrive. These conditions will make it challenging to predict shifts in each of the components of seasonal weather conditions. Our proposed system also aims to give a solution that can be adapted to other vineyards as well.


Author(s):  
Dandan Li ◽  
Zhiqiang Zuo ◽  
Yijing Wang

Using an event-based switching law, we address the stability issue for continuous-time switched affine systems in the network environment. The state-dependent switching law in terms of the region function is firstly developed. We combine the region function with the event-triggering mechanism to construct the switching law. This can provide more candidates for the selection of the next activated subsystem at each switching instant. As a result, it is possible for us to activate the appropriate subsystem to avoid the sliding motion. The Zeno behavior for the switched affine system can be naturally ruled out by guaranteeing a positive minimum inter-event time between two consecutive executions of the event-triggering threshold. Finally, two numerical examples are given to demonstrate the effectiveness of the proposed method.


2021 ◽  
pp. 073401682110383
Author(s):  
Bruno Truzzi ◽  
Marcelo Justus ◽  
Henrique C. Kawamura ◽  
Thomas V. Conti

This article investigates the relationship between the perception of violence and the spending on security goods and services in households. Individual microdata from a random national survey on family budget carried out in Brazil in 2008-2009 were used for modeling the household spending using two instrumental variables. The stability of results was checked by applying the Lasso-Gaussian regularization method in the selection of the statistically significant variables. Positive relationships were found between household spending on security goods and services and (i) the fear of insecurity at the household level, (ii) the neighbors’ spending on security, and (iii) the registered criminality, but no evidence was found on the relationship between the role of police on household spending on security goods and services.


2016 ◽  
Vol 22 (2) ◽  
pp. 324-341
Author(s):  
Rafał Gawałkiewicz ◽  
Anna Szafarczyk

Mounds, as anthropogenic constructions of a very delicate structure, are subdued to constant changes, which, due to the impact of external factors (prolonged precipitation, tremors) are subdued to deformations in the form of mass movements. These phenomena usually have the character of mild soil creep in time and sometimes, as a result of rapid loss of stability, they are seriously damaged by landslide. This phenomenon causes temporary exclusion of the object from use. In the framework of the protection of these objects, the maintenance was carried out within the preventive measures referring to the construction and surveying monitoring of the geometry changes in time, as a result of phenomena taking place in the ground medium under the influence of environmental factors causing strains. The process of the deformation of mounds is similar to the characteristic, according to the Terzagie's theory. The application of surveying technologies of high precision allows the monitoring of changes in their geometry in time. The properly defined study area and the proper selection of measurement technology in the aspect of the accuracy of the prediction of changes, can efficiently help in defining the scale of deformations in the decisive process referring to the way of efficient protection of barrows. The article presents the results of point monitoring carried out with surveying technologies within 11 measurement series carried out on the selected measurement base of the Wanda Mound. The use of measurement technologies of integrated and specialist software, allows complex assessment of the degree of deformation and the trends of these changes in time, as well as identifying anomaly zones in the framework of the landslide monitoring.


2020 ◽  
Vol 86 (5) ◽  
Author(s):  
O. E. López ◽  
L. Guazzotto

The present work considers the stability of a high- $\beta$ , large aspect ratio, circular plasma with diffuse profiles for the safety factor and the angular toroidal frequency (López & Guazzotto, Phys. Plasmas, vol. 24, 032501). An application of the Frieman–Rotenberg formalism results in a system of scalar eigenmode equations whose coupling is retained at the plasma–vacuum transition but is disregarded across the plasma column, which is a standard practice. The solution technique consists of a multidimensional shooting method for the poloidal harmonics; robust initial guesses are constructed by solving the dispersion relation in the static scenario with vanishing magnetic shear. Flow shear appears as a high- $\beta$ toroidal contribution, and we illustrate its destabilizing influence on $n=1$ external kink modes in the presence of ideal and resistive walls. Internal resonances are avoided by means of the selection of appropriate equilibrium parameters. The stabilizing influence of a finite positive average magnetic shear is also exemplified.


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