Role of VDR in 1α,25-dihydroxyvitamin D3-dependent non-genomic activation of MAPKs, Src and Akt in skeletal muscle cells

2013 ◽  
Vol 136 ◽  
pp. 125-130 ◽  
Author(s):  
Claudia Buitrago ◽  
Verónica Gonzalez Pardo ◽  
Ricardo Boland
Keyword(s):  
Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Dihydroxyvitamin D3

scholarly journals Zinc Modulates Several Transcription-Factor Regulated Pathways in Mouse Skeletal Muscle Cells

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5098
Author(s):  
Parisa Vahidi Ferdowsi ◽  
Rachel Ng ◽  
John Adulcikas ◽  
Sukhwinder Singh Sohal ◽  
Stephen Myers
Keyword(s):  
Skeletal Muscle ◽  
Transcription Factor ◽  
Metal Ion ◽  
Insulin Signalling ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Western Blots ◽  
Insulin Signalling Pathway ◽  
C2c12 Skeletal Muscle Cells

Zinc is an essential metal ion involved in many biological processes. Studies have shown that zinc can activate several molecules in the insulin signalling pathway and the concomitant uptake of glucose in skeletal muscle cells. However, there is limited information on other potential pathways that zinc can activate in skeletal muscle. Accordingly, this study aimed to identify other zinc-activating pathways in skeletal muscle cells to further delineate the role of this metal ion in cellular processes. Mouse C2C12 skeletal muscle cells were treated with insulin (10 nM), zinc (20 µM), and the zinc chelator TPEN (various concentrations) over 60 min. Western blots were performed for the zinc-activation of pAkt, pErk, and pCreb. A Cignal 45-Reporter Array that targets 45 signalling pathways was utilised to test the ability of zinc to activate pathways that have not yet been described. Zinc and insulin activated pAkt over 60 min as expected. Moreover, the treatment of C2C12 skeletal muscle cells with TPEN reduced the ability of zinc to activate pAkt and pErk. Zinc also activated several associated novel transcription factor pathways including Nrf1/Nrf2, ATF6, CREB, EGR1, STAT1, AP-1, PPAR, and TCF/LEF, and pCREB protein over 120 min of zinc treatment. These studies have shown that zinc’s activity extends beyond that of insulin signalling and plays a role in modulating novel transcription factor activated pathways. Further studies to determine the exact role of zinc in the activation of transcription factor pathways will provide novel insights into this metal ion actions.

scholarly journals Induction of utrophin gene expression by heregulin in skeletal muscle cells: Role of the N-box motif and GA binding protein

1999 ◽  
Vol 96 (6) ◽  
pp. 3223-3227 ◽  
Author(s):  
A. O. Gramolini ◽  
L. M. Angus ◽  
L. Schaeffer ◽  
E. A. Burton ◽  
J. M. Tinsley ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Binding Protein ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Ga Binding Protein

scholarly journals Defining the role of mesenchymal stromal cells on the regulation of matrix metalloproteinases in skeletal muscle cells

2014 ◽  
Vol 323 (2) ◽  
pp. 297-313 ◽  
Author(s):  
Chiara Sassoli ◽  
Daniele Nosi ◽  
Alessia Tani ◽  
Flaminia Chellini ◽  
Benedetta Mazzanti ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Matrix Metalloproteinases ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Muscle Cells ◽  
Skeletal Muscle Cells
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