Investigation of Free Radical Scavenging Enzyme Activities and Lipid Peroxidation in Human Placental Tissues With Miscarriage

2006 ◽  
Vol 13 (5) ◽  
pp. 384-388 ◽  
Author(s):  
Aydan Biri ◽  
Mustafa Kavutcu ◽  
Nuray Bozkurt ◽  
Erdinç Devrim ◽  
Nilhan Nurlu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Enzyme Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging

Antioxidant and Hepatoprotective Effects of Orthosiphon stamineus Benth.

2007 ◽  
Vol 35 (01) ◽  
pp. 115-126 ◽  
Author(s):  
Mun Fei Yam ◽  
Rusliza Basir ◽  
Mohd. Zaini Asmawi ◽  
Zhari Ismail
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Liver Toxicity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Liver Function Tests ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Orthosiphon Stamineus

Orthosiphon stamineus (OS), Benth. (Lamiaceae) is widely used in Malaysia for treatments of various kidney and liver ailments. In the experiment, DPPH• radicals scavenging, Fe 3+-induced lipid peroxidation inhibiting activities and trolox equivalent antioxidant capacity (TEAC) of methanol/water extract of Orthosiphon stamineus (SEOS) were determined. The results indicated that SEOS exhibited antioxidant, lipid peroxidation inhibition and free radical scavenging activities. The hepatoprotective activity of the SEOS was studied using CCl 4-induced liver toxicity in rats. The activity was assessed by monitoring liver function tests through the measurement of alanine transaminase (ALT) and aspartate transaminase (AST). Furthermore, hepatic tissues were also subjected to histopathological studies. Pretreatment of SEOS (125, 250, 500 and 1000 mg/kg p.o.) dose-dependently reduced the necrotic changes in rat liver and inhibited the increase of serum ALT and AST activities. The results of the present study indicated that the hepatoprotective effect of Orthosiphon stamineus might be ascribable to its antioxidant and free radical scavenging property.

scholarly journals Constituents of the Stem of Nauclea orientalis

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001 ◽  
Author(s):  
Phan Thi Anh Dao ◽  
Tran Le Quan ◽  
Nguyen Thi Thanh Mai
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Spectroscopic Data ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Propanoic Acid ◽  
Coumaric Acid ◽  
Aloe Emodin ◽  
Inhibitory Activities

A new α-pinene derivative (1) was isolated from the stem of Nauclea orientalis (L.) L. (Rubiaceae), together with twelve known compounds, including four terpenoids, loganetin (2), loganin (3), sweroside (4), grandifloroside (5), four simple phenols, methyl protocatechuate (6), trans- p-coumaric acid (7), 3-(2,4-dihydroxylphenyl)propanoic acid (8), methyl 3-(2,4-dihydroxylphenyl)propanoate (9), two coumarin glucosides, skimmin (10), adicardin (11), an anthraquinone, aloe emodin (12), and a lignan, (+)-pinoresinol (13). Their structures were elucidated on the basis of spectroscopic data. All compounds were tested for DPPH free radical scavenging and lipid peroxidation inhibitory activities. Among them, compounds 5 and 6 displayed strong antioxidant activity with IC50 values of 6.6 and 67.9 μM, 12.4 and 813.0 μM, respectively.

scholarly journals Free Radical Scavenging Activity, Reducing Power and Anti-Hemolytic Capacity of Algerian Drimia maritima Baker Flower Extracts

2020 ◽  
Vol 10 (4) ◽  
pp. 70-78
Author(s):  
Abir Rezzagui ◽  
Abderrahmane Senator ◽  
Soumia Benbrinis ◽  
Hamama Bouriche
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Superoxide Anion ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Scavenging Activity

This study was undertaken to evaluate the antioxidant and anti-hemolytic properties of Algerian Drimia maritima Baker flower extracts. Determination of phenolic content was carried out to estimate the chemical composition of D. maritima extracts. Antioxidant properties were investigated in all extracts using free radical scavenging activity (against DPPH, ABTS, hydroxyl radical, and superoxide anion), reducing power, inhibition of lipid peroxidation, and anti-hemolytic capacity. Phenolic determination revealed that D. maritima flowers contain phenolic compounds, flavonoids, and tannins. Ethyl acetate extract showed the highest reducing power and scavenging activity using DPPH and ABTS assays. However, aqueous extract was the most effective against hydroxyl radical, superoxide anion, and lipid peroxidation. The half-time of hemolysis indicates that chloroform extract exhibited the best anti-hemolytic capacity in the AAPH induced hemolysis model. The results of this study suggest that D. maritima could be used as a possible source of antioxidant phenolic compounds and that further determination of these compounds may provide more information on their medicinal value.  Keywords: Drimia maritima, phenolic compounds, scavenging activity, reducing power, anti-hemolytic.

scholarly journals In vitro inhibitory potential of avocado fruits, Persea americana (Lauraceae) against oxidation, inflammation and key enzymes linked to skin diseases

2021 ◽  
Vol 69 (2) ◽  
Author(s):  
Muhammed M. Hürkul ◽  
Sezen Yilmaz Sarialtin ◽  
Ayşegül Köroğlu ◽  
Tülay Çoban
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Skin Diseases ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Persea Americana ◽  
Methanol Extracts ◽  
Hexane Extracts ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Activities

Avocado (Persea americana Mill.) is a member of Lauraceae with one-seeded berry fruit and cultivated in all tropical, subtropical regions in the world and in the Southern coast region of Turkey. Oxidative damage caused by UV can trigger inflammation, resulting in serious inflammatory skin diseases including eczema, seborrheic dermatitis, hyperpigmentation and ageing. Enzyme inhibitors involved in melanogenesis, such as tyrosinase, have been used recently for hyperpigmentation and skin diseases in cosmetic products. Objective: This study aimed to evaluate the antioxidant, anti-inflammatory, anti-tyrosinase activities and total polyphenolic contents of the different parts of P. americana fruit. Methods: The fruit was divided into exocarp, mesocarp, seed, and then methanol and n-hexane extracts were prepared. DPPH and ABTS free radical scavenging capacities and inhibitory potentials on lipid peroxidation were determined to investigate the antioxidant potentials of the extracts. Anti-inflammatory activities of the extracts were evaluated by measuring the stabilization level of the human red blood cell membrane. The tyrosinase inhibitory activities of the samples were determined using mushroom tyrosinase. Results: In general methanol extracts possessed remarkable higher DPPH free radical scavenging activities than n-hexane extracts. The highest activity was determined in methanol extracts of seed (4.17 ± 0.04 mg/mL) followed by exocarp (5.25 ± 0.05 mg/mL). Overall methanol extracts possessed higher ABTS free radical scavenging activities than n-hexane extracts. The greatest ABTS free radical scavenging activity was obtained in methanol extracts of seed (0.03 ± 0.01 mg/mL). In the anti-lipid peroxidation assay, the greatest activity was noticed in methanol extracts of seed (7.71 ± 0.36 µg/mL) followed by exocarp (12.12 ± 0.34 µg/mL), while all n-hexane extracts were inactive. Overall methanol extracts exhibited higher anti-inflammatory and antioxidant properties than n-hexane extracts. However, the maximum anti-tyrosinase activity was determined in n-hexane extracts of exocarp (0.40 ± 0.01 mg/mL) followed by seed (0.46 ± 0.01 mg/mL). Conclusions: These extracts are promising candidates for use as natural products-based antioxidant and anti-inflammatory properties in inflammation-related disease, and also anti-tyrosinase properties in dermatological applications.

scholarly journals Evaluation of the Free Radical Scavenging Activities of Ellagic Acid and Ellagic Acid Peracetate by EPR Spectrometry

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4800
Author(s):  
Ajit Kumar ◽  
Preeti Kaushik ◽  
Sandra Incerpi ◽  
Jens Z. Pedersen ◽  
Sanjay Goel ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Free Radical ◽  
Ellagic Acid ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Cellular Systems ◽  
Ros Production ◽  
Microsomal Lipid Peroxidation

The purpose of this study was to examine the free radical scavenging and antioxidant activities of ellagic acid (EA) and ellagic acid peracetate (EAPA) by measuring their reactions with the radicals, 2,2-diphenyl-1-picrylhydrazyl and galvinoxyl using EPR spectroscopy. We have also evaluated the influence of EA and EAPA on the ROS production in L-6 myoblasts and rat liver microsomal lipid peroxidation catalyzed by NADPH. The results obtained clearly indicated that EA has tremendous ability to scavenge free radicals, even at concentration of 1 µM. Interestingly even in the absence of esterase, EAPA, the acetylated product of EA, was also found to be a good scavenger but at a relatively slower rate. Kinetic studies revealed that both EA and EAPA have ability to scavenge free radicals at the concentrations of 1 µM over extended periods of time. In cellular systems, EA and EAPA were found to have similar potentials for the inhibition of ROS production in L-6 myoblasts and NADPH-dependent catalyzed microsomal lipid peroxidation.

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