MP44-01 A PROGNOSTIC MODEL FOR OVERALL SURVIVAL IN PATIENTS WITH METASTATIC CLEAR CELL RENAL CARCINOMA: RESULTS FROM CALGB 90206 (ALLIANCE)

Hypoxia is a significant clinical feature and regulates various tumor processes in clear cell renal carcinoma (ccRCC). Increasing evidence has demonstrated that long non-coding RNAs (lncRNAs) are closely associated with the survival outcomes of ccRCC patients and regulates hypoxia-induced tumor processes. Thus, this study aimed to develop a hypoxia-related lncRNA (HRL) prognostic model for predicting the survival outcomes in ccRCC. LncRNAs in ccRCC samples were extracted from The Cancer Genome Atlas database. Hypoxia-related genes were downloaded from the Molecular Signatures Database. A co-expression analysis between differentially expressed lncRNAs and hypoxia-related genes in ccRCC samples was performed to identify HRLs. Univariate and multivariate Cox regression analyses were performed to select nine optimal lncRNAs for developing the HRL model. The prognostic model showed good performance in predicting prognosis among patients with ccRCC, and the validation sets reached consistent results. The model was also found to be related to the clinicopathologic parameters of tumor grade and tumor stage and to tumor immune infiltration. In conclusion, our findings indicate that the hypoxia-lncRNA assessment model may be useful for prognostication in ccRCC cases. Furthermore, the nine HRLs included in the model might be useful targets for investigating the tumorigenesis of ccRCC and designing individualized treatment strategies.

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Clear cell renal carcinoma - evolution of metastatic disease and overall survival after four lines of targeted therapy. Case report

Oncolog-Hematolog ro ◽

10.26416/onhe.41.4.2017.1349 ◽

2017 ◽

Vol 4 (41) ◽

pp. 32

Author(s):

Andreea Lăzescu ◽

Laura Ciurea ◽

Ioan Burlănescu ◽

Dragoş Mitulescu ◽

Alexandru C. Grigorescu

Keyword(s):

Overall Survival ◽

Case Report ◽

Targeted Therapy ◽

Metastatic Disease ◽

Renal Carcinoma ◽

Clear Cell ◽

Clear Cell Renal Carcinoma ◽

Cell Renal Carcinoma

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Low expression of ferritinophagy-related NCOA4 gene in relation to unfavorable outcome and defective immune cells infiltration in clear cell renal carcinoma

BMC Cancer ◽

10.1186/s12885-020-07726-z ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Yanhua Mou ◽

Jinchun Wu ◽

Yao Zhang ◽

Omar Abdihamid ◽

Chaojun Duan ◽

...

Keyword(s):

Overall Survival ◽

Immune Cells ◽

Renal Carcinoma ◽

Clear Cell ◽

Gene Expression Omnibus ◽

The Cancer Genome Atlas ◽

Clear Cell Renal Carcinoma ◽

Cell Renal Carcinoma ◽

Cancer Genome Atlas ◽

Genome Atlas

Abstract Background Clear cell renal cell carcinoma is susceptible to ferroptosis, and immunotherapy is recently recommended as a priority for the initial treatment of metastatic clear cell renal carcinoma. Increased ferroptosis and immune activation can synergistically reinforce each other in killing cancer cells. NCOA4 depletion can eliminate iron accumulation and thus weaken ferroptosis. Here, we aim to identify and validate the association between NCOA4 expression, clinicopathologic characteristics, and overall survival in ccRCC by using The Cancer Genome Atlas and Gene Expression Omnibus databases. We further analyze the interacted proteins of NCOA4 and infiltrated immune cells via TIMER and GEPIA databases. Methods NCOA4 expression in clear cell renal carcinoma (ccRCC) tissues and normal adjacent tissues in The Cancer Genome Atlas (TCGA) data were primarily screened, and further validated in another independent cohort from the gene expression omnibus (GEO) database and human protein atlas. The relationships of NCOA4 expression and clinicopathologic parameters and overall survival (OS) were assessed using multivariate methods and Kaplan-Meier survival curves. And the proteins network with which NCOA4 interacted were also built using the online STRING website. Meanwhile, we use TIMER and GEPIA databases to investigate the relationships between NCOA4 expression and infiltrated immune cells and their corresponding gene marker sets. Results Contrast to normal tissue, NCOA4 expression was lower in ccRCC tumor tissue(p < 0.05). Lower NCOA4 expression was closely associated with high-grade malignancy and advanced TNM stage. Univariate and multivariate analysis indicated the overall survival of ccRCC cases with low NCOA4 level is shorter than those of patients with high NCOA4 expression (p < 0.05). FTL and FTH1 were the important proteins interacting with NCOA4. ccRCC with NCOA4 deficiency presented the paucity of infiltrated immune cells and their matching marker sets, including CD8+ T cells. Conclusion Deficient NCOA4 expression was related to disease progression and poor prognosis, as well as impaired infiltration of immune cells in ccRCC.

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A Molecular Model for Predicting Overall Survival in Patients with Metastatic Clear Cell Renal Carcinoma: Results from CALGB 90206 (Alliance)

EBioMedicine ◽

10.1016/j.ebiom.2015.09.012 ◽

2015 ◽

Vol 2 (11) ◽

pp. 1814-1820 ◽

Cited By ~ 4

Author(s):

Hyung L. Kim ◽

Susan Halabi ◽

Ping Li ◽

Greg Mayhew ◽

Jeff Simko ◽

...

Keyword(s):

Overall Survival ◽

Renal Carcinoma ◽

Clear Cell ◽

Molecular Model ◽

Clear Cell Renal Carcinoma ◽

Cell Renal Carcinoma

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Vimentin over-expression and carbonic anhydrase IX under-expression are independent predictors of recurrence, specific and overall survival in non-metastatic clear-cell renal carcinoma: a validation study

World Journal of Urology ◽

10.1007/s00345-016-1854-y ◽

2016 ◽

Vol 35 (1) ◽

pp. 81-87 ◽

Cited By ~ 15

Author(s):

A. Ingels ◽

M. Hew ◽

F. Algaba ◽

O. J. de Boer ◽

R. J. A. van Moorselaar ◽

...

Keyword(s):

Overall Survival ◽

Carbonic Anhydrase ◽

Validation Study ◽

Renal Carcinoma ◽

Clear Cell ◽

Carbonic Anhydrase Ix ◽

Clear Cell Renal Carcinoma ◽

Over Expression ◽

Predictors Of Recurrence ◽

Cell Renal Carcinoma

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1651: Molecular Prognostic Modeling Using Protein Expression Profile in Clear Cell Renal Carcinoma

The Journal of Urology ◽

10.1016/s0022-5347(18)38859-1 ◽

2004 ◽

Vol 171 (4S) ◽

pp. 436-436 ◽

Cited By ~ 3

Author(s):

Hyung L. Kim ◽

David B. Seligson ◽

Nicolette Janzen ◽

Matthew H. Bui ◽

Robert A. Figlin ◽

...

Keyword(s):

Protein Expression ◽

Expression Profile ◽

Renal Carcinoma ◽

Clear Cell ◽

Clear Cell Renal Carcinoma ◽

Protein Expression Profile ◽

Cell Renal Carcinoma ◽

Prognostic Modeling

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The VHL tumor suppressor inhibits expression of the IGF1R and its loss induces IGF1R upregulation in human clear cell renal carcinoma

Oncogene ◽

10.1038/sj.onc.1210474 ◽

2007 ◽

Vol 26 (45) ◽

pp. 6499-6508 ◽

Cited By ~ 68

Author(s):

J S P Yuen ◽

M E Cockman ◽

M Sullivan ◽

A Protheroe ◽

G D H Turner ◽

...

Keyword(s):

Tumor Suppressor ◽

Renal Carcinoma ◽

Clear Cell ◽

Clear Cell Renal Carcinoma ◽

Cell Renal Carcinoma

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The Influence of the Extremely Low Frequency Electromagnetic Field on Clear Cell Renal Carcinoma

International Journal of Molecular Sciences ◽

10.3390/ijms22031342 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1342

Author(s):

Aleksandra Cios ◽

Martyna Ciepielak ◽

Wanda Stankiewicz ◽

Łukasz Szymański

Keyword(s):

Electromagnetic Field ◽

Cancer Cells ◽

Cell Lines ◽

Renal Carcinoma ◽

Clear Cell ◽

Low Frequency ◽

Inhibitory Effect ◽

Hek293 Cells ◽

Clear Cell Renal Carcinoma ◽

Cell Renal Carcinoma

The development of new technologies and industry is conducive to the increase in the number and variety of electromagnetic field (EMF) sources in our environment. The main sources of EMF are high-voltage lines, household appliances, audio/video devices, mobile phones, radio stations, and radar devices. In the growing use of electronic devices, scientists are increasingly interested in the effects of EMF on human health. Even though many studies on the effects of EMF have already been carried out, none of them has shown a significant effect on mammals, including humans. Moreover, it is not entirely clear how EMF influences cell behavior. The International Agency for Research on Cancer on 31 May 2011, classified PEM as a possible carcinogenic factor. This study aimed to investigate the effect of the electromagnetic field on morphological and functional changes in clear cell renal carcinoma. The research was carried out on in vitro cultures of four cell lines: HEK293, 786-O 769-P, and Caki1. The results of the research showed that the EMF of low frequency had a slight effect on the viability of cells. EMF, which induced cell arrest in the G1 phase, increased the number of early apoptotic cells and decreased the number of viable cells in the 786-O line. EMF did not affect the proliferation and viability of HEK293 cells. Extreme low-frequency EMF (ELF-EMF) also showed an inhibitory effect on the migration and metastatic properties of clear cell kidney cancer cells. Moreover, shortly after the end of ELF-EMF exposure, significant increases in ROS levels were observed in all tested cell lines. As part of the work, it was shown that low-frequency EMF shows an inhibitory effect on the proliferation of primary cancer cells, diminishing their migratory, invasive, and metastatic abilities. It also increases the apoptosis of cancer cells and the amount of reactive oxygen species. Based on the results of our research, we want to point up that the effect of ELF-EMF depends on a specific metabolic state or at a specific stage in the cell cycle of the cells under study.

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