A rapid and sensitive assay for detection of replication-competent adenoviruses by a combination of microcarrier cell culture and quantitative PCR

2007 ◽  
Vol 145 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Johanna A.C. Schalk ◽  
Claudette G.J.C.A. de Vries ◽  
Tom J.H. Orzechowski ◽  
Marianne G. Rots
Keyword(s):  
Cell Culture ◽  
Quantitative Pcr ◽  
Sensitive Assay ◽  
Microcarrier Cell Culture

Fructose as a carbohydrate source yields stable pH and redox parameters in microcarrier cell culture

1982 ◽  
Vol 124 (2) ◽  
pp. 353-358 ◽  
Author(s):  
Toshiko Imamura ◽  
Charles L. Crespi ◽  
William G. Thilly ◽  
Henri Brunengraber
Keyword(s):  
Cell Culture ◽  
Carbohydrate Source ◽  
Microcarrier Cell Culture

Microcarrier cell culture: Vero cells on cytodex® 1

1986 ◽  
Vol 9 (4) ◽  
pp. 205-210
Author(s):  
U. Lindskog ◽  
B. Lundgren ◽  
I. Wergeland ◽  
D. Billig
Keyword(s):  
Cell Culture ◽  
Vero Cells ◽  
Microcarrier Cell Culture

scholarly journals Assessment of Long-Term Effects of Nanoparticles in a Microcarrier Cell Culture System

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56791 ◽  
Author(s):  
Maria Mrakovcic ◽  
Markus Absenger ◽  
Regina Riedl ◽  
Claudia Smole ◽  
Eva Roblegg ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Cell Culture System ◽  
Long Term Effects ◽  
Microcarrier Cell Culture

Retention of Rotavirus Infectivity in Mussels Heated by Using the French Recipe Moules Marinières

2015 ◽  
Vol 78 (11) ◽  
pp. 2064-2069 ◽  
Author(s):  
DORIS SOBRAL MARQUES SOUZA ◽  
TAKAYUKI MIURA ◽  
CÉCILE LE MENNEC ◽  
CÉLIA REGINA MONTE BARARDI ◽  
FRANÇOISE S. LE GUYADER
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Quantitative Pcr ◽  
Infectious Virus ◽  
Real Time Quantitative Pcr ◽  
Infectivity Assay ◽  
A Cell

To evaluate the persistence of infectious virus after heating, mussels contaminated with a rotavirus strain were prepared following the French recipe moules marinières (mariner's mussels). Rotavirus was then quantified by real-time quantitative PCR (RT-qPCR) and a cell culture infectivity assay. Results showed the persistence of infectious virus after 3 min of cooking. After 5 min, when no infectious virus could be detected, the RT-qPCR approach showed a 1-log decrease compared with concentrations detected after 1 min of cooking.

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