Development of reverse transcription loop-mediated isothermal amplification for rapid detection of H9 avian influenza virus

2008 ◽  
Vol 151 (2) ◽  
pp. 200-203 ◽  
Author(s):  
Hao-tai Chen ◽  
Jie Zhang ◽  
De-hui Sun ◽  
Li-na Ma ◽  
Xiang-tao Liu ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

scholarly journals Rapid Detection of Subtype H10N8 Influenza Virus by One-Step Reverse Transcription–Loop-Mediated Isothermal Amplification Methods

2015 ◽  
Vol 53 (12) ◽  
pp. 3884-3887 ◽  
Author(s):  
Hongmei Bao ◽  
Xiaoxiao Feng ◽  
Yong Ma ◽  
Jianzhong Shi ◽  
Yuhui Zhao ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Detection Limit ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
High Sensitivity ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Live Poultry ◽  
One Step ◽  
Live Poultry Markets

We developed hemagglutinin- and neuraminidase-specific one-step reverse transcription–loop-mediated isothermal amplification assays for detecting the H10N8 virus. The detection limit of the assays was 10 copies of H10N8 virus, and the assays did not amplify nonspecific RNA. The assays can detect H10N8 virus from chicken samples with high sensitivity and specificity, and they can serve as an effective tool for detecting and monitoring H10N8 virus in live poultry markets.

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