scholarly journals The stability of HIV-1 nucleic acid in whole blood and improved detection of HIV-1 in alternative specimen types when compared to Dried Blood Spot (DBS) specimens

2018 ◽  
Vol 261 ◽  
pp. 91-97 ◽  
Author(s):  
Cheryl Jennings ◽  
Brian Harty ◽  
Salvatore R. Scianna ◽  
Suzanne Granger ◽  
Amy Couzens ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Whole Blood ◽  
Dried Blood Spot ◽  
The Stability ◽  
Alternative Specimen ◽  
Hiv 1 ◽  
Blood Spot

scholarly journals Volumetric Absorptive Microsampling of Blood for Untargeted Lipidomics

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 262
Author(s):  
Camilla Marasca ◽  
Maria Encarnacion Blanco Arana ◽  
Michele Protti ◽  
Andrea Cavalli ◽  
Laura Mercolini ◽  
...  
Keyword(s):  
High Resolution ◽  
Whole Blood ◽  
Chemical Space ◽  
Solid Support ◽  
Dried Blood Spot ◽  
Proof Of Concept ◽  
Viable Option ◽  
Human Whole Blood ◽  
Blood Spot ◽  
Volumetric Absorptive Microsampling

In the present, proof-of-concept paper, we explore the potential of one common solid support for blood microsampling (dried blood spot, DBS) and a device (volumetric absorptive microsampling, VAMS) developed for the untargeted lipidomic profiling of human whole blood, performed by high-resolution LC-MS/MS. Dried blood microsamples obtained by means of DBS and VAMS were extracted with different solvent compositions and compared with fluid blood to evaluate their efficiency in profiling the lipid chemical space in the most broad way. Although more effort is needed to better characterize this approach, our results indicate that VAMS is a viable option for untargeted studies and its use will bring all the corresponding known advantages in the field of lipidomics, such as haematocrit independence.

scholarly journals Dried Blood Spot RNA Transcriptomes Correlate with Transcriptomes Derived from Whole Blood RNA

2018 ◽  
Vol 98 (5) ◽  
pp. 1541-1546 ◽  
Author(s):  
Mary J. Reust ◽  
Myung Hee Lee ◽  
Jenny Xiang ◽  
Wei Zhang ◽  
Dong Xu ◽  
...  
Keyword(s):  
Whole Blood ◽  
Dried Blood Spot ◽  
Blood Spot

Development and validation of a dried blood spot–HPLC assay for the determination of metronidazole in neonatal whole blood samples

2010 ◽  
Vol 397 (2) ◽  
pp. 687-693 ◽  
Author(s):  
Maysa Faisal Suyagh ◽  
Godwill Iheagwaram ◽  
Prashant Laxman Kole ◽  
Jeff Millership ◽  
Paul Collier ◽  
...  
Keyword(s):  
Whole Blood ◽  
Dried Blood Spot ◽  
Hplc Assay ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
Development And Validation ◽  
Blood Spot

scholarly journals A simple assay for glutathione in whole blood

The Analyst ◽  
2015 ◽  
Vol 140 (10) ◽  
pp. 3339-3342 ◽  
Author(s):  
Lovemore Hakuna ◽  
Brandon Doughan ◽  
Jorge O. Escobedo ◽  
Robert M. Strongin
Keyword(s):  
Fluorescence Detection ◽  
Whole Blood ◽  
Fast Response ◽  
Dried Blood Spot ◽  
Blood Spot

A simple dried blood spot isolation protocol yields glutathione samples that are free from optical interferents. Fluorescence detection and quantitation is achieved using a fast response probe selective for glutathione.

scholarly journals Protein Bead Array for the Detection of HIV-1 Antibodies from Fresh Plasma and Dried-Blood-Spot Specimens

2004 ◽  
Vol 50 (7) ◽  
pp. 1250-1253 ◽  
Author(s):  
Sylvie Faucher ◽  
Alexandre Martel ◽  
Alice Sherring ◽  
Tao Ding ◽  
Laurie Malloch ◽  
...  
Keyword(s):  
Dried Blood Spot ◽  
Fresh Plasma ◽  
Hiv 1 ◽  
Blood Spot

Evaluation of the dried blood spot filter paper technology and five testing strategies of HIV-1 and HIV-2 infections in West Africa

2006 ◽  
Vol 38 (11-12) ◽  
pp. 1050-1056 ◽  
Author(s):  
Ramu Sarge-Njie ◽  
Maarten Schim Van Der Loeff ◽  
Saihou Ceesay ◽  
David Cubitt ◽  
Saihou Sabally ◽  
...  
Keyword(s):  
West Africa ◽  
Filter Paper ◽  
Dried Blood Spot ◽  
Testing Strategies ◽  
Paper Technology ◽  
Hiv 1 ◽  
Blood Spot

scholarly journals Impact of incomplete coverage of neonatal dried blood spot screening on estimating HIV-1 seroprevalence

1996 ◽  
Vol 117 (1) ◽  
pp. 173-177 ◽  
Author(s):  
E. J. Hutchinson ◽  
A. Streetly ◽  
C. Grant ◽  
R. Pollitt ◽  
P. Eldridge ◽  
...  
Keyword(s):  
Census Data ◽  
Monitoring Programme ◽  
Dried Blood Spot ◽  
Black African ◽  
Child Bearing ◽  
Incomplete Coverage ◽  
Substantial Bias ◽  
Minimal Bias ◽  
Hiv 1 ◽  
Blood Spot

SummaryThe aim of this study was to determine the extent to which selective under-coverage of births to mothers more likely to be at risk of HIV-1 infection will result in a significant underestimation of the true neonatal seroprevalence. Census data, local birth statisties, maternity data and data from the prevalence monitoring programme were used to produce a model to predict the effects of under-coverage in the uptake of neonatal metabolic screening which has been observed in babies with a mother of ethnic group black African. The adjustment factor which allows for under-coverage is the relative inclusion ratio (RIR); the probability that samples from a group at different risk of HIV infection were included in the survey divided by the probability of inclusion for samples from all other babies. The RIR was found to be close to unity (0·97), indicating a minimal bias. Under usual conditions only if the relative inclusion ratio (RIR) declined to values of 0·87 or below would there be a substantial bias. Despite some selective under representation, the results obtained from the Unlinked Anonymous HIV Monitoring Programme Dried Blood Spot Survey would seem to identity levels of prevalence in the population of child-bearing women with a good degree of accuracy and remains a useful tool for resource allocation, planning of services, provision of care and counselling.

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