Volumetric Absorptive Microsampling of Blood for Untargeted Lipidomics

In the present, proof-of-concept paper, we explore the potential of one common solid support for blood microsampling (dried blood spot, DBS) and a device (volumetric absorptive microsampling, VAMS) developed for the untargeted lipidomic profiling of human whole blood, performed by high-resolution LC-MS/MS. Dried blood microsamples obtained by means of DBS and VAMS were extracted with different solvent compositions and compared with fluid blood to evaluate their efficiency in profiling the lipid chemical space in the most broad way. Although more effort is needed to better characterize this approach, our results indicate that VAMS is a viable option for untargeted studies and its use will bring all the corresponding known advantages in the field of lipidomics, such as haematocrit independence.

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Volumetric absorptive microsampling (VAMS) coupled with high-resolution, accurate-mass (HRAM) mass spectrometry as a simplified alternative to dried blood spot (DBS) analysis for therapeutic drug monitoring of cardiovascular drugs

Clinical Mass Spectrometry ◽

10.1016/j.clinms.2018.08.002 ◽

2018 ◽

Vol 10 ◽

pp. 1-8 ◽

Cited By ~ 5

Author(s):

Sangeeta Tanna ◽

Ahmed Alalaqi ◽

Dennis Bernieh ◽

Graham Lawson

Keyword(s):

Mass Spectrometry ◽

High Resolution ◽

Therapeutic Drug Monitoring ◽

Drug Monitoring ◽

Dried Blood Spot ◽

Accurate Mass ◽

Therapeutic Drug ◽

Blood Spot ◽

Volumetric Absorptive Microsampling ◽

High Resolution Accurate Mass

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Dried Blood Spot RNA Transcriptomes Correlate with Transcriptomes Derived from Whole Blood RNA

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.17-0653 ◽

2018 ◽

Vol 98 (5) ◽

pp. 1541-1546 ◽

Cited By ~ 3

Author(s):

Mary J. Reust ◽

Myung Hee Lee ◽

Jenny Xiang ◽

Wei Zhang ◽

Dong Xu ◽

...

Keyword(s):

Whole Blood ◽

Dried Blood Spot ◽

Blood Spot

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The stability of HIV-1 nucleic acid in whole blood and improved detection of HIV-1 in alternative specimen types when compared to Dried Blood Spot (DBS) specimens

Journal of Virological Methods ◽

10.1016/j.jviromet.2018.08.009 ◽

2018 ◽

Vol 261 ◽

pp. 91-97 ◽

Cited By ~ 2

Author(s):

Cheryl Jennings ◽

Brian Harty ◽

Salvatore R. Scianna ◽

Suzanne Granger ◽

Amy Couzens ◽

...

Keyword(s):

Nucleic Acid ◽

Whole Blood ◽

Dried Blood Spot ◽

The Stability ◽

Alternative Specimen ◽

Hiv 1 ◽

Blood Spot

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Development and validation of a dried blood spot–HPLC assay for the determination of metronidazole in neonatal whole blood samples

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-010-3571-5 ◽

2010 ◽

Vol 397 (2) ◽

pp. 687-693 ◽

Cited By ~ 42

Author(s):

Maysa Faisal Suyagh ◽

Godwill Iheagwaram ◽

Prashant Laxman Kole ◽

Jeff Millership ◽

Paul Collier ◽

...

Keyword(s):

Whole Blood ◽

Dried Blood Spot ◽

Hplc Assay ◽

Blood Samples ◽

Whole Blood Samples ◽

Development And Validation ◽

Blood Spot

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Tranexamic acid quantification in human whole blood using liquid samples or volumetric absorptive microsampling devices

Bioanalysis ◽

10.4155/bio-2020-0088 ◽

2020 ◽

Author(s):

Elodie Lamy ◽

Ileana Runge ◽

Ian Roberts ◽

Haleema Shakur-Still ◽

Stanislas Grassin-Delyle

Keyword(s):

Tranexamic Acid ◽

Whole Blood ◽

International Association ◽

Clinical Samples ◽

European Medicines Agency ◽

Therapeutic Drug ◽

Blood Samples ◽

Human Whole Blood ◽

Liquid Samples ◽

Volumetric Absorptive Microsampling

Background: Recent clinical trials demonstrate the benefits of the antifibrinolytic drug tranexamic acid but its pharmacokinetics remain to be investigated more in depth. Although pharmacokinetics studies are usually performed with plasma, volumetric absorptive microsampling devices allow us to analyze dried whole blood samples with several advantages. Materials & methods: High-sensitivity LC–MS/MS methods for the quantification of tranexamic acid in human whole blood using liquid samples or dry samples on volumetric absorptive microsampling devices were developed and validated based on International Association from Therapeutic Drug Monitoring and Clinical Toxicology, European Medicines Agency and US Food and Drug Administration guidance. Conclusion: The method performances were excellent across the range of clinically relevant concentrations. The stability of tranexamic acid in blood samples stored up to 1 month at +50°C was demonstrated. The methods’ suitability was confirmed with clinical samples.

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A simple assay for glutathione in whole blood

The Analyst ◽

10.1039/c5an00345h ◽

2015 ◽

Vol 140 (10) ◽

pp. 3339-3342 ◽

Cited By ~ 13

Author(s):

Lovemore Hakuna ◽

Brandon Doughan ◽

Jorge O. Escobedo ◽

Robert M. Strongin

Keyword(s):

Fluorescence Detection ◽

Whole Blood ◽

Fast Response ◽

Dried Blood Spot ◽

Blood Spot

A simple dried blood spot isolation protocol yields glutathione samples that are free from optical interferents. Fluorescence detection and quantitation is achieved using a fast response probe selective for glutathione.

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Automated high-capacity on-line extraction and bioanalysis of dried blood spot samples using liquid chromatography/high-resolution accurate mass spectrometry

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.7033 ◽

2014 ◽

Vol 28 (22) ◽

pp. 2415-2426 ◽

Cited By ~ 20

Author(s):

Regina V. Oliveira ◽

Jack Henion ◽

Enaksha R. Wickremsinhe

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

High Resolution ◽

High Capacity ◽

Dried Blood Spot ◽

Line Extraction ◽

On Line ◽

Accurate Mass Spectrometry ◽

Blood Spot ◽

High Resolution Accurate Mass

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Quantitative determination of opioids in whole blood using fully automated dried blood spot desorption coupled to on-line SPE-LC-MS/MS

Drug Testing and Analysis ◽

10.1002/dta.1927 ◽

2015 ◽

Vol 8 (1) ◽

pp. 30-38 ◽

Cited By ~ 42

Author(s):

Ruth Verplaetse ◽

Jack Henion

Keyword(s):

Quantitative Determination ◽

Whole Blood ◽

Dried Blood Spot ◽

On Line ◽

Blood Spot

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Novel LC–MS/MS method for the determination of selumetinib (AZD6244) in whole blood collected with volumetric absorptive microsampling

Bioanalysis ◽

10.4155/bio-2020-0062 ◽

2020 ◽

Vol 12 (13) ◽

pp. 883-892

Author(s):

Ramakrishna R Voggu ◽

Theodore S Brus ◽

Chineta T Barksdale ◽

Paul Severin ◽

Patricia Hansen ◽

...

Keyword(s):

Whole Blood ◽

Ammonium Hydroxide ◽

Bioanalytical Method Validation ◽

Simple Method ◽

Human Whole Blood ◽

Regulatory Guidance ◽

Validation Parameters ◽

Diagnostic Applications ◽

Volumetric Absorptive Microsampling

Aim: A method has been developed and validated for quantitation of selumetinib in human whole blood collected using a Mitra™ volumetric absorptive microsampling device. This device is patient-friendly, affording less-invasive sampling with broad applicability to clinical and diagnostic applications – specifically in pediatric populations. Materials & methods: In this method, drug is extracted from the Mitra device via sonication in methanol: Ammonium hydroxide, then analyzed by LC–MS/MS. The linear range for selumetinib analysis is 2.00–2000 ng/ml. Results: All validation parameters met acceptance criteria established in agreement with current regulatory guidance for bioanalytical method validation. The stability of selumetinib in Mitra tips was established at both ambient and frozen conditions. Conclusion: A simple method has been developed and validated for determination of selumetinib from human whole blood, collected using volumetric absorptive microsampling and analyzed by LC–MS/MS.

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