Protein Bead Array for the Detection of HIV-1 Antibodies from Fresh Plasma and Dried-Blood-Spot Specimens

SummaryThe aim of this study was to determine the extent to which selective under-coverage of births to mothers more likely to be at risk of HIV-1 infection will result in a significant underestimation of the true neonatal seroprevalence. Census data, local birth statisties, maternity data and data from the prevalence monitoring programme were used to produce a model to predict the effects of under-coverage in the uptake of neonatal metabolic screening which has been observed in babies with a mother of ethnic group black African. The adjustment factor which allows for under-coverage is the relative inclusion ratio (RIR); the probability that samples from a group at different risk of HIV infection were included in the survey divided by the probability of inclusion for samples from all other babies. The RIR was found to be close to unity (0·97), indicating a minimal bias. Under usual conditions only if the relative inclusion ratio (RIR) declined to values of 0·87 or below would there be a substantial bias. Despite some selective under representation, the results obtained from the Unlinked Anonymous HIV Monitoring Programme Dried Blood Spot Survey would seem to identity levels of prevalence in the population of child-bearing women with a good degree of accuracy and remains a useful tool for resource allocation, planning of services, provision of care and counselling.

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Long-term stability of HIV-1 antibody in dried blood spot samples and eluates

AIDS ◽

10.1097/qad.0000000000000348 ◽

2014 ◽

Vol 28 (12) ◽

pp. 1850-1851 ◽

Cited By ~ 2

Author(s):

David Williams ◽

Pat Tookey ◽

Catherine S. Peckham ◽

Mario Cortina-Borja

Keyword(s):

Dried Blood Spot ◽

Term Stability ◽

Long Term Stability ◽

Hiv 1 ◽

Blood Spot

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Low prevalence of transmitted HIV-1 drug resistance detected by a dried blood spot (DBS)-based next-generation sequencing (NGS) method in newly diagnosed individuals in Cameroon in the years 2015–16

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dky103 ◽

2018 ◽

Vol 73 (7) ◽

pp. 1917-1929 ◽

Cited By ~ 8

Author(s):

Herbert A Mbunkah ◽

Alex Marzel ◽

Stefan Schmutz ◽

Yik Lim Kok ◽

Osvaldo Zagordi ◽

...

Keyword(s):

Drug Resistance ◽

Next Generation Sequencing ◽

Dried Blood Spot ◽

Newly Diagnosed ◽

Next Generation ◽

Low Prevalence ◽

Next Generation Sequencing Ngs ◽

Generation Sequencing ◽

Hiv 1 ◽

Blood Spot

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Evaluation of dried blood spot specimens for HIV-1 drug-resistance testing using the Trugene® HIV-1 genotyping assay

Journal of Clinical Virology ◽

10.1016/j.jcv.2007.12.011 ◽

2008 ◽

Vol 41 (4) ◽

pp. 283-287 ◽

Cited By ~ 36

Author(s):

Renee Hallack ◽

Lauren E. Doherty ◽

Judith A. Wethers ◽

Monica M. Parker

Keyword(s):

Drug Resistance ◽

Dried Blood Spot ◽

Resistance Testing ◽

Drug Resistance Testing ◽

Genotyping Assay ◽

Hiv 1 ◽

Blood Spot

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Factors influencing HIV-1 banding patterns in miniaturized Western blot testing of dried blood spot specimens

Journal of Immunological Methods ◽

10.1016/0022-1759(92)90196-z ◽

1992 ◽

Vol 154 (2) ◽

pp. 225-233 ◽

Cited By ~ 2

Author(s):

Timothy C. Granade ◽

Susan K. Phillips ◽

Carol J. Bell ◽

Chou-Pong Pau ◽

Bharat Parekh ◽

...

Keyword(s):

Western Blot ◽

Dried Blood Spot ◽

Banding Patterns ◽

Factors Influencing ◽

Western Blot Testing ◽

Hiv 1 ◽

Blood Spot

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Dried blood spot specimen quality and validation of a new pre-analytical processing method for qualitative HIV-1 PCR, KwaZulu-Natal, South Africa

African Journal of Laboratory Medicine ◽

10.4102/ajlm.v5i1.349 ◽

2016 ◽

Vol 5 (1) ◽

Cited By ~ 6

Author(s):

Kerusha Govender ◽

Raveen Parboosing ◽

Ntombizandile Siyaca ◽

Pravikrishnen Moodley

Keyword(s):

South Africa ◽

Limit Of Detection ◽

Health Facilities ◽

Dried Blood Spot ◽

Specimen Preparation ◽

Kwazulu Natal ◽

Specimen Processing ◽

Specimen Quality ◽

Hiv 1 ◽

Blood Spot

Background: Poor quality dried blood spot (DBS) specimens are usually rejected by virology laboratories, affecting early infant diagnosis of HIV. The practice of combining two incompletely-filled DBS in one specimen preparation tube during pre-analytical specimen processing (i.e., the two-spot method) has been implemented to reduce the number of specimens being rejected for insufficient volume.Objectives: This study analysed laboratory data to describe the quality of DBS specimens and the use of the two-spot method over a one-year period, then validated the two-spot method against the standard (one-spot) method.Methods: Data on HIV-1 PCR test requests submitted in 2014 to the Department of Virology at Inkosi Albert Luthuli Central Hospital in KwaZulu-Natal province, South Africa were analysed to describe reasons for specimen rejection, as well as results of the two-spot method. The accuracy, lower limit of detection and precision of the two-spot method were assessed.Results: Of the 88 481 specimens received, 3.7% were rejected for pre-analytical problems. Of those, 48.9% were rejected as a result of insufficient specimen volume. Two health facilities had significantly more specimen rejections than other facilities. The two-spot method prevented 10 504 specimen rejections. The Pearson correlation coefficient comparing the standard to the two-spot method was 0.997.Conclusions: The two-spot method was comparable with the standard method of pre-analytical specimen processing. Two health facilities were identified for targeted retraining on specimen quality. The two-spot method of DBS specimen processing can be used as an adjunct to retraining, to reduce the number of specimens rejected and improve linkage to care.

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