Comment on the article “Wastewater treatment performance and microbial community of anode electrodes of membrane and membrane-less MFCs under effect of sunlight” by Dao Anh Nguyen, Ngoc Pham, Hoa Thi Pham [Journal of Water Process Engineering 42 (2021) 102159]

AbstractThe present study points to the relevance of the earthworms-microorganism’s symbiotic and synergistic interactions that drive the wastewater treatment by identifying the most essential mechanisms underlying the removal of contaminants during vermifiltration technology. Previous studies have showed the presence of earthworms improves treatment performance of vermifilter (VF), but earthworm microbial community dynamics, their structure and functional characteristics in VF were not fully investigated. To investigate the effects of earthworms on the inherent microbial community of the VF, the present study envisages the dynamics of the complex symbiosis of earthworms & microorganisms associated to the treatment mechanisms. In this study, the design, operations and performance evaluation and influent, effluent and filter media layer were investigated for microbial diversity inside the earthworm population, along with the antimicrobial activity, enzymatic activity, and protein profiling assays. The results showed that earthworm gut microbial communities were dominated by Gammaproteobacteria, and the percentages arrived to 59–60% of the microbial species detected, while filter media layer showed presence of Firmicutes and Actinobacteria. The protein profiling of the microbiota associated with the VF showed that earthworms feeding and earthworm–microorganism interaction were responsible for enhanced treatment performance. The finding provides an insight into the complex earthworm microbial dynamics and mechanisms for wastewater treatment in VF. Furthermore, earthworm predation strongly regulated microbial biomass while improving microbial activity, and is deciphered as the possible mechanisms behind the vermifiltration technology.

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Integrating sulfur, iron(II), and fixed organic carbon for mixotrophic denitrification in a composite filter bed reactor for decentralized wastewater treatment: Performance and microbial community

The Science of The Total Environment ◽

10.1016/j.scitotenv.2021.148825 ◽

2021 ◽

pp. 148825

Author(s):

Xiejuan Lu ◽

Yanlei Wan ◽

Zhenxing Zhong ◽

Bo Liu ◽

Feixiang Zan ◽

...

Keyword(s):

Wastewater Treatment ◽

Microbial Community ◽

Organic Carbon ◽

Decentralized Wastewater Treatment ◽

Treatment Performance ◽

Composite Filter ◽

Filter Bed

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Gas-lift anaerobic dynamic membrane bioreactors for high strength synthetic wastewater treatment: Effect of biogas sparging velocity and HRT on treatment performance

Chemical Engineering Journal ◽

10.1016/j.cej.2016.02.003 ◽

2016 ◽

Vol 305 ◽

pp. 46-53 ◽

Cited By ~ 30

Author(s):

Mustafa Evren Ersahin ◽

Juan B. Gimenez ◽

Hale Ozgun ◽

Yu Tao ◽

Henri Spanjers ◽

...

Keyword(s):

Wastewater Treatment ◽

Treatment Effect ◽

High Strength ◽

Membrane Bioreactors ◽

Synthetic Wastewater ◽

Dynamic Membrane ◽

Treatment Performance ◽

Gas Lift

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Pollutant-Removing Biofilter Strains Associated with High Ammonia and Hydrogen Sulfide Removal Rate in a Livestock Wastewater Treatment Facility

Sustainability ◽

10.3390/su13137358 ◽

2021 ◽

Vol 13 (13) ◽

pp. 7358

Author(s):

Dong-Hyun Kim ◽

Hyun-Sik Yun ◽

Young-Saeng Kim ◽

Jong-Guk Kim

Keyword(s):

Wastewater Treatment ◽

Hydrogen Sulfide ◽

Microbial Community ◽

Microbial Communities ◽

Illumina Miseq ◽

Illumina Miseq Sequencing ◽

Miseq Sequencing ◽

Treatment Facility ◽

Livestock Wastewater ◽

Wastewater Treatment Facility

This study analyzed the microbial community metagenomically to determine the cause of the functionality of a livestock wastewater treatment facility that can effectively remove pollutants, such as ammonia and hydrogen sulfide. Illumina MiSeq sequencing was used in analyzing the composition and structure of the microbial community, and the 16S rRNA gene was used. Through Illumina MiSeq sequencing, information such as diversity indicators as well as the composition and structure of microbial communities present in the livestock wastewater treatment facility were obtained, and differences between microbial communities present in the investigated samples were compared. The number of reads, operational taxonomic units, and species richness were lower in influent sample (NLF), where the wastewater enters, than in effluent sample (NL), in which treated wastewater is found. This difference was greater in June 2019 than in January 2020, and the removal rates of ammonia (86.93%) and hydrogen sulfide (99.72%) were also higher in June 2019. In both areas, the community composition was similar in January 2020, whereas the influent sample (NLF) and effluent sample (NL) areas in June 2019 were dominated by Proteobacteria (76.23%) and Firmicutes (67.13%), respectively. Oleiphilaceae (40.89%) and Thioalkalibacteraceae (12.91%), which are related to ammonia and hydrogen sulfide removal, respectively, were identified in influent sample (NLF) in June 2019. They were more abundant in June 2019 than in January 2020. Therefore, the functionality of the livestock wastewater treatment facility was affected by characteristics, including the composition of the microbial community. Compared to Illumina MiSeq sequencing, fewer species were isolated and identified in both areas using culture-based methods, suggesting Illumina MiSeq sequencing as a powerful tool to determine the relevance of microbial communities for pollutant removal.

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