Role of fractalkine/CX3CR1 signaling pathway in the recovery of neurological function after early ischemic stroke in a rat model

Hyperuricemia is closely related to acute ischemic stroke (AIS). In our study, we investigated the pattern of miRNA-155-5p and miRNA-124-5p expressions along with its clinical application in AIS and hyperuricemia patients and in a hyperuricemia rat model by RT-qPCR. The hyperuricemia rat model was established, and we found that the levels of miRNA-155-5p and miRNA-124-5p were increased in the serum, brain and kidney tissues compared with those in the normal rats. We proved that the levels of miRNA-155-5p and miRNA-124-5p were also elevated in AIS, hyperuricemia and AIS accompanied with hyperuricemia patients enrolled from the department of neurology in Inner Mongolia People’s Hospital (IMPH). The miRNA-155-5p and miRNA-124-5p were mainly associated with neuronal apoptosis, cerebral vasospasm, neuron projection, neuron projection morphogenesis, neuron differentiation and exocytosis. The above results might provide clues for the study the pathogenesis of AIS and hyperuricemia.

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Inhibition of the SOCS1-JAK2-STAT3 Signaling Pathway Confers Neuroprotection in Rats with Ischemic Stroke

Cellular Physiology and Biochemistry ◽

10.1159/000484585 ◽

2017 ◽

Vol 44 (1) ◽

pp. 85-98 ◽

Cited By ~ 21

Author(s):

Xiao-Lei Wang ◽

Chun-Mei Qiao ◽

Jiong-Ou Liu ◽

Chun-Yang Li

Keyword(s):

Ischemic Stroke ◽

Protein Expression ◽

Cell Viability ◽

Signaling Pathway ◽

Rat Model ◽

Caspase 3 ◽

Neuronal Cells ◽

Stat3 Signaling ◽

Stat3 Signaling Pathway ◽

Mrna And Protein Expression

Background: The present study aims to investigate the protective effects of the SOCS1-JAK2-STAT3 signaling pathway on neurons in a rat model of ischemic stroke. Methods: Our study was conducted using an ischemic stroke rat model. After the microglia were extracted, 40 neonatal Sprague-Dawley (SD) rats were assigned into the blank, AG490, model and negative control (NC) groups. The neurological function of all the rats was evaluated. Histopathological changes were observed. qRT-PCR and western blotting were applied to measure the expression of genes and proteins in the SOCS1-JAK2-STAT3 signaling pathway and related to apoptosis. The TUNEL assay was conducted to calculate the cellular morphology and apoptosis of neuronal cells. Cell viability was detected using the MTT assay. In addition, immunoassays were used to measure the content of superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) as well as the levels of oxidative stress. Results: Compared with the blank group, the model and NC groups showed higher neurological function scores—the cytoplasm of the neurons were cavitated, the organelles were reduced with unclear margins, some of the neurons were necrotic, and apoptosis was increased. In addition, the NC and model groups exhibited decreased cell viability, lower mRNA and protein expression of SOCS1 SOCS3 and bcl-2 and reduced SOD and GSH levels but higher mRNA and protein expression levels of AK2, STAT3,Bax and caspase-3 as well as increased protein expression of P-JAK2, P-STAT3 and activated caspase-3 (c-caspase-3). Moreover, the MDA levels were up-regulated in the NC and model groups. In contrast, opposing trends were found in the AG490 group compared with the NC and model groups. Conclusion: These data demonstrate that inhibiting the SOCS1-JAK2-STAT3 signaling pathway can reduce the loss of nerve function and apoptosis of neuronal cells, which provides a new target for the clinical treatment of ischemic stroke.

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Protective Role of Apocynin via Suppression of Neuronal Autophagy and TLR4/NF-κB Signaling Pathway in a Rat Model of Traumatic Brain Injury

Neurochemical Research ◽

10.1007/s11064-017-2372-z ◽

2017 ◽

Vol 42 (11) ◽

pp. 3296-3309 ◽

Cited By ~ 18

Author(s):

Yan Feng ◽

Changmeng Cui ◽

Xin Liu ◽

Qiang Wu ◽

Fuguang Hu ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

Signaling Pathway ◽

Rat Model ◽

Protective Role ◽

Neuronal Autophagy

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Based on Dll4/Notch1 Signaling Pathway to Elucidate the Therapeutic Effect and Mechanism of L-borneolum on Cerebral Ischemic Stroke Rats

10.21203/rs.3.rs-847271/v1 ◽

2021 ◽

Author(s):

Taiwei Dong ◽

Nian Chen ◽

Rong Ma ◽

Qian Xie ◽

Xiaoqing Guo ◽

...

Keyword(s):

Ischemic Stroke ◽

Signaling Pathway ◽

Caspase 3 ◽

Neurological Function ◽

Immunohistochemical Method ◽

Research Progress ◽

Notch1 Signaling ◽

Cerebral Ischemic Stroke ◽

Cerebral Ischemic ◽

Notch1 Signaling Pathway

Abstract Background: The current research progress suggests that a single therapy may not be ideal means for complex cerebral ischemic stoke (CIS). l-Borneolum is the crystallization of fresh leaves of Blumea balsamifera (L.) DC, we have found that l-borneolum plays a best anti-cerebral ischemic effect than d-borneolum or synthetic borneolum. However, the mechanism is needed to be explored in depth. Therefore, based on comprehensive approach that combines molecular docking technology and molecular biology, this stiudy aimed to investigate the potential mechanism of l-borneolum on CIS rats and provide scientific evidence for the treatment of l-borneolum in CIS.Methods: Cerebral ischemic stroke (CIS) rats with permanent middle cerebral artery occlusion (pMCAO) were applied to this study. The modified neurological severity scores (mNSS) and Longa neurological function scoring methods were used to assess the neurobehavioral scores. 2,3,5-Triphenyltetrazolium chloride (TTC) staining and hematoxylin-eosin (HE) staining were used to evaluate pathological changes of cerebral tissue. Ultrastructure of cortical capillary and blood-brain barrier (BBB) in rats were observed by transmission electron microscopy. In addition, the protein expression of Notch1, Dll4, Hey1, Hes1, Hes5, VEGFA and p65 in the cortex of rats were determined by Western blotting (WB). The protein contents of Caspase 3 in the cortex of rats were determined by immunohistochemical method (IHC). Results: l-Borneolum could prolong the resuscitation time, reduce the abnormal increased rectal temperature, improve neurological function in a dose-dependently. Additionally, l-borneolum could significantly alleviate brainstem edema and inflammation, as well as improve the ultrastructure of capillary and BBB in cortex. Moreover, 0.2 g/kg l-borneolum could substantially decrease the protein expressions of Dll4, Notch1, Hes1, Hes5, and VEGFA in the cortex while it decreased the level of Caspase-3 in the cortex of rats. Conclusions: l-Borneolum could repair neurological function by regulating Dll4/Notch1 signaling pathway, l-borneolum might be a good complementary agent for CIS.

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Role of the extracellular signal-regulated kinase 1/2 signaling pathway in regulating the secretion of bronchial smooth muscle cells in a rat model of chronic asthma

Chinese Medical Journal ◽

10.1097/00029330-200801010-00014 ◽

2008 ◽

Vol 121 (1) ◽

pp. 73-77 ◽

Cited By ~ 5

Author(s):

Min XIE ◽

Xian-sheng LIU ◽

Yong-jian XU ◽

Zhen-xiang ZHANG ◽

Jing BAI ◽

...

Keyword(s):

Smooth Muscle ◽

Smooth Muscle Cells ◽

Signaling Pathway ◽

Rat Model ◽

Chronic Asthma ◽

Bronchial Smooth Muscle ◽

Extracellular Signal Regulated Kinase ◽

Extracellular Signal ◽

Bronchial Smooth Muscle Cells

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PROTECTIVE ROLE OF XINNAONING TABLET IN ISCHEMIC STROKE IN RAT MODEL

African Journal of Traditional Complementary and Alternative Medicines ◽

10.21010/ajtcam.v15i3.10 ◽

2018 ◽

Vol 15 (3) ◽

pp. 84-90

Author(s):

Ling Wang ◽

Hongdi Lv ◽

Guoqi Xie ◽

Feng Su ◽

Xiuli Geng ◽

...

Keyword(s):

Ischemic Stroke ◽

Rat Model ◽

Protective Role

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Inhibition of SNK-SPAR signaling pathway promotes the restoration of motor function in a rat model of ischemic stroke

Journal of Cellular Biochemistry ◽

10.1002/jcb.26278 ◽

2017 ◽

Vol 119 (1) ◽

pp. 1093-1110 ◽

Cited By ~ 5

Author(s):

Qing-Yu Fan ◽

Jing-Jie Liu ◽

Gui-Lian Zhang ◽

Hai-Qin Wu ◽

Ru Zhang ◽

...

Keyword(s):

Ischemic Stroke ◽

Signaling Pathway ◽

Motor Function ◽

Rat Model

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PROTECTIVE ROLE OF XINNAONING TABLET IN ISCHEMIC STROKE IN RAT MODEL

African Journal of Traditional Complementary and Alternative Medicines ◽

10.21010/ajtcamv15i3.10 ◽

2018 ◽

Vol 15 (3) ◽

pp. 84

Author(s):

Ling Wang ◽

Hongdi Lv ◽

Guoqi Xie ◽

Feng Su ◽

Xiuli Geng ◽

...

Keyword(s):

Ischemic Stroke ◽

Cerebral Ischemia ◽

Rat Model ◽

Brain Function ◽

Protective Role ◽

Serum Samples ◽

Male Wistar Rats ◽

Wet Weight

Background: Stroke has been considered as the second leading cause of death worldwide. The survivors of stroke experience different level of impair brain function. In China, Chinese traditional medicine had been widely accepted for stroke therapy and prevention. In this study, we developed Traditional Chinese Medicine based Xinnaoning (peace of heart and brain) Tablet and tested its protective role for ischemic stroke in rat model. Material and Methods: Male Wistar rats (n=60) with 12 weeks old and weight from 180 to 200 gram were randomly divided to five groups (n=12). For the groups with Xinaoning administration, the drug was administrated to rats once per day for 7 consecutive days. The blood clotting time and the thrombus wet weight was measured. Serum samples were collected from each rat for further Measurement of biochemical indicators. Results: Our results demonstrated that Xinnaoning tablet reduced lactate acid (LD) level and increased lactic acid dehydrogenase (LDH) in cerebral ischemia model as well as reduced the infarct size caused by stroke. Besides, evaluation of the level of different ATPases suggested Xinnaoning tablet could modulate ATPases activity and confer a protective role in brain. Moreover, analysis indicated Xinnaoning tablet have the anti-coagulation effect in vivo which may contribute to the protection of ischemia. Conclusion: Our findings suggest that Xinnaoning tablet may be a potential way for cerebral ischemia prevention.

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