The interaction of a β2 adrenoceptor agonist drug with biomimetic cell membrane models: The case of terbutaline sulphate

Lipid vesicles, especially giant lipid vesicles (GLVs), are usually adopted as cell membrane models and their preparation has been widely studied. However, the effects of some nonelectrolytes on GLV formation have not been specifically studied so far. In this paper, the effects of the nonelectrolytes, including sucrose, glucose, sorbitol and ethanol, and their coexistence with sodium chloride, on the lipid hydration and GLV formation were investigated. With the hydration method, it was found that the sucrose, glucose and sorbitol showed almost the same effect. Their presence in the medium enhanced the hydrodynamic force on the lipid membranes, promoting the GLV formation. GLV formation was also promoted by the presence of ethanol with ethanol volume fraction in the range of 0 to 20 percent, but higher ethanol content resulted in failure of GLV formation. However, the participation of sodium chloride in sugar solution and ethanol solution stabilized the lipid membranes, suppressing the GLV formation. In addition, the ethanol and the sodium chloride showed the completely opposite effects on lipid hydration. These results could provide some suggestions for the efficient preparation of GLVs.

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Quantum dot effects upon the interaction between porphyrins and phospholipids in cell membrane models

European Biophysics Journal ◽

10.1007/s00249-015-1088-8 ◽

2015 ◽

Vol 45 (3) ◽

pp. 219-227 ◽

Cited By ~ 2

Author(s):

Gustavo G. Parra ◽

Galina Borissevitch ◽

Iouri Borissevitch ◽

Ana P. Ramos

Keyword(s):

Cell Membrane ◽

Quantum Dot ◽

Membrane Models

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Antituberculosis Drug Interactions with Membranes: A Biophysical Approach Applied to Bedaquiline

Membranes ◽

10.3390/membranes9110141 ◽

2019 ◽

Vol 9 (11) ◽

pp. 141

Author(s):

Marina Pinheiro ◽

Heinz Amenitsch ◽

Salette Reis

Keyword(s):

Cell Membrane ◽

Eukaryotic Cell ◽

Membrane Model ◽

Molar Ratio ◽

X Ray ◽

X Ray Scattering ◽

Membrane Models ◽

Bacterial Cell Membrane ◽

Cell Membrane Model ◽

Ray Scattering

This work focuses on the interaction of the novel and representative antituberculosis (anti-TB) drug bedaquiline (BDQ) with different membrane models of eukaryotic and prokaryotic cells. The effect of BDQ on eukaryotic cell membrane models was assessed using liposomes, namely, multilamellar vesicles (MLVs) made of 1,2-dimyristoyl-rac-glycero-3-phosphocholine (DMPC) and also a mixture of DMPC and cholesterol (CHOL) (8:2 molar ratio). To mimic the prokaryotic cell membrane, 1,2-dimyristoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DMPG) and 1,1′2,2′-tetra-oleoyl-cardiolipin (TOCL) were chosen. Powerful biophysical techniques were employed, including small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS), to understand the effect of BDQ on the nanostructure of the membrane models. The results showed that BDQ demonstrated a pronounced disordering effect in the bacterial cell membrane models, especially in the membrane model with cardiolipin (CL), while the human cell membrane model with large fractions of neutral phospholipids remained less affected. The membrane models and techniques provide detailed information about different aspects of the drug–membrane interaction, thus offering valuable information to better understand the effect of BDQ on their target membrane-associated enzyme as well as its side effects on the cardiovascular system.

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Interaction of chitosan derivatives with cell membrane models in a biologically relevant medium

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2020.111048 ◽

2020 ◽

Vol 192 ◽

pp. 111048 ◽

Cited By ~ 1

Author(s):

Rafael de Oliveira Pedro ◽

Andressa Ribeiro Pereira ◽

Osvaldo N. Oliveira ◽

Paulo Barbeitas Miranda

Keyword(s):

Cell Membrane ◽

Chitosan Derivatives ◽

Biologically Relevant ◽

Membrane Models

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The interaction of eugenol with cell membrane models at the air–water interface is modulated by the lipid monolayer composition

Biophysical Chemistry ◽

10.1016/j.bpc.2015.07.007 ◽

2015 ◽

Vol 207 ◽

pp. 7-12 ◽

Cited By ~ 7

Author(s):

Giulia E.G. Gonçalves ◽

Fernanda S. de Souza ◽

João Henrique G. Lago ◽

Luciano Caseli

Keyword(s):

Cell Membrane ◽

Lipid Monolayer ◽

Water Interface ◽

Air Water Interface ◽

Membrane Models

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Interaction of Chitosan with Cell Membrane Models at the Air−Water Interface

Biomacromolecules ◽

10.1021/bm0701550 ◽

2007 ◽

Vol 8 (5) ◽

pp. 1633-1640 ◽

Cited By ~ 79

Author(s):

Felippe J. Pavinatto ◽

Adriana Pavinatto ◽

Luciano Caseli ◽

David S. dos Santos, ◽

Thatyane M. Nobre ◽

...

Keyword(s):

Cell Membrane ◽

Water Interface ◽

Air Water Interface ◽

Membrane Models

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Localization and interactions of melatonin in dry cholesterol/lecithin mixed reversed micelles used as cell membrane models

Journal of Pineal Research ◽

10.1111/j.1600-079x.2005.00211.x ◽

2005 ◽

Vol 38 (4) ◽

pp. 292-298 ◽

Cited By ~ 28

Author(s):

David Bongiorno ◽

Leopoldo Ceraulo ◽

Mirella Ferrugia ◽

Felice Filizzola ◽

Angela Ruggirello ◽

...

Keyword(s):

Cell Membrane ◽

Reversed Micelles ◽

Membrane Models

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Recent advances in the use of Langmuir monolayers as cell membrane models

Eclética Química Journal ◽

10.26850/1678-4618eqj.v46.1si.2021.p18-29 ◽

2021 ◽

Vol 46 (1SI) ◽

pp. 18-29

Author(s):

Andressa Ribeiro Pereira ◽

Osvaldo Novais de Oliveira Junior

Keyword(s):

Cell Membrane ◽

Review Paper ◽

Molecular Level ◽

Langmuir Monolayers ◽

Pharmaceutical Drugs ◽

Membrane Composition ◽

Biologically Relevant ◽

Membrane Models ◽

Biologically Relevant Molecules

Understanding the role of biomolecules in cells at the molecular level has been the trade of Prof. Marcio Francisco Colombo and Prof. Jo�o Ruggiero Neto in their carriers, which is why it was found appropriate to address the use of Langmuir monolayers as cell membrane models in this special issue. In the review paper, we elaborate upon the reasons why Langmuir monolayers are good models with the possible control of membrane composition and molecular packing. After describing several experimental methods to characterize the Langmuir monolayers, we discuss selected results from the last five years where monolayers were made to interact with pharmaceutical drugs, emerging pollutants and other biologically-relevant molecules. The challenges to take the field forward are also commented upon.

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