Quantum dot effects upon the interaction between porphyrins and phospholipids in cell membrane models

2015 ◽  
Vol 45 (3) ◽  
pp. 219-227 ◽  
Author(s):  
Gustavo G. Parra ◽  
Galina Borissevitch ◽  
Iouri Borissevitch ◽  
Ana P. Ramos
Keyword(s):  
Cell Membrane ◽  
Quantum Dot ◽  
Membrane Models

scholarly journals Formation of Giant Lipid Vesicles in the Presence of Nonelectrolytes—Glucose, Sucrose, Sorbitol and Ethanol

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 945
Author(s):  
Qiong Wang ◽  
Ning Hu ◽  
Jincan Lei ◽  
Qiurong Qing ◽  
Jing Huang ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Cell Membrane ◽  
Lipid Membranes ◽  
Volume Fraction ◽  
Hydrodynamic Force ◽  
Lipid Vesicles ◽  
Ethanol Solution ◽  
Sugar Solution ◽  
Ethanol Content ◽  
Membrane Models

Lipid vesicles, especially giant lipid vesicles (GLVs), are usually adopted as cell membrane models and their preparation has been widely studied. However, the effects of some nonelectrolytes on GLV formation have not been specifically studied so far. In this paper, the effects of the nonelectrolytes, including sucrose, glucose, sorbitol and ethanol, and their coexistence with sodium chloride, on the lipid hydration and GLV formation were investigated. With the hydration method, it was found that the sucrose, glucose and sorbitol showed almost the same effect. Their presence in the medium enhanced the hydrodynamic force on the lipid membranes, promoting the GLV formation. GLV formation was also promoted by the presence of ethanol with ethanol volume fraction in the range of 0 to 20 percent, but higher ethanol content resulted in failure of GLV formation. However, the participation of sodium chloride in sugar solution and ethanol solution stabilized the lipid membranes, suppressing the GLV formation. In addition, the ethanol and the sodium chloride showed the completely opposite effects on lipid hydration. These results could provide some suggestions for the efficient preparation of GLVs.

scholarly journals Antituberculosis Drug Interactions with Membranes: A Biophysical Approach Applied to Bedaquiline

Membranes ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 141
Author(s):  
Marina Pinheiro ◽  
Heinz Amenitsch ◽  
Salette Reis
Keyword(s):  
Cell Membrane ◽  
Eukaryotic Cell ◽  
Membrane Model ◽  
Molar Ratio ◽  
X Ray ◽  
X Ray Scattering ◽  
Membrane Models ◽  
Bacterial Cell Membrane ◽  
Cell Membrane Model ◽  
Ray Scattering

This work focuses on the interaction of the novel and representative antituberculosis (anti-TB) drug bedaquiline (BDQ) with different membrane models of eukaryotic and prokaryotic cells. The effect of BDQ on eukaryotic cell membrane models was assessed using liposomes, namely, multilamellar vesicles (MLVs) made of 1,2-dimyristoyl-rac-glycero-3-phosphocholine (DMPC) and also a mixture of DMPC and cholesterol (CHOL) (8:2 molar ratio). To mimic the prokaryotic cell membrane, 1,2-dimyristoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DMPG) and 1,1′2,2′-tetra-oleoyl-cardiolipin (TOCL) were chosen. Powerful biophysical techniques were employed, including small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS), to understand the effect of BDQ on the nanostructure of the membrane models. The results showed that BDQ demonstrated a pronounced disordering effect in the bacterial cell membrane models, especially in the membrane model with cardiolipin (CL), while the human cell membrane model with large fractions of neutral phospholipids remained less affected. The membrane models and techniques provide detailed information about different aspects of the drug–membrane interaction, thus offering valuable information to better understand the effect of BDQ on their target membrane-associated enzyme as well as its side effects on the cardiovascular system.

Interaction of chitosan derivatives with cell membrane models in a biologically relevant medium

2020 ◽  
Vol 192 ◽  
pp. 111048 ◽  
Author(s):  
Rafael de Oliveira Pedro ◽  
Andressa Ribeiro Pereira ◽  
Osvaldo N. Oliveira ◽  
Paulo Barbeitas Miranda
Keyword(s):  
Cell Membrane ◽  
Chitosan Derivatives ◽  
Biologically Relevant ◽  
Membrane Models

Graphene quantum dot assisted translocation of drugs into a cell membrane

Nanoscale ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 4503-4514 ◽  
Author(s):  
Zhengyang Xue ◽  
Quan Sun ◽  
Li Zhang ◽  
Zhengzhong Kang ◽  
Lijun Liang ◽  
...  
Keyword(s):  
Free Energy ◽  
Cell Membrane ◽  
Quantum Dot ◽  
Lipid Bilayer ◽  
Graphene Quantum Dot ◽  
A Cell

Translocation free energy of model drugs permeating into the lipid bilayer could be significantly reduced with the assistance of GQDs.

The interaction of eugenol with cell membrane models at the air–water interface is modulated by the lipid monolayer composition

2015 ◽  
Vol 207 ◽  
pp. 7-12 ◽  
Author(s):  
Giulia E.G. Gonçalves ◽  
Fernanda S. de Souza ◽  
João Henrique G. Lago ◽  
Luciano Caseli
Keyword(s):  
Cell Membrane ◽  
Lipid Monolayer ◽  
Water Interface ◽  
Air Water Interface ◽  
Membrane Models

In situ labelling chemistry of respiratory syncytial viruses by employing the biotinylated host-cell membrane protein for tracking the early stage of virus entry

2014 ◽  
Vol 50 (99) ◽  
pp. 15776-15779 ◽  
Author(s):  
Lin Ling Zheng ◽  
Xiao Xi Yang ◽  
Yue Liu ◽  
Xiao Yan Wan ◽  
Wen Bi Wu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Protein ◽  
Quantum Dot ◽  
Early Stage ◽  
Virus Entry ◽  
Host Cells ◽  
Host Cell Membrane ◽  
Respiratory Syncytial Viruses ◽  
Cell Membrane Protein

Anin situstrategy for producing quantum dot-labelled respiratory syncytial viruses by incorporating the biotinylated membrane protein of the host cells into mature virions is reported.

Dual-color quantum dot detection of a heterotetrameric potassium channel (hKCa3.1)

2011 ◽  
Vol 300 (4) ◽  
pp. C843-C849 ◽  
Author(s):  
Daniel E. J. Waschk ◽  
Anke Fabian ◽  
Thomas Budde ◽  
Albrecht Schwab
Keyword(s):  
Quantum Dots ◽  
Plasma Membrane ◽  
Membrane Potential ◽  
Cell Membrane ◽  
Quantum Dot ◽  
Fluorescence Microscopy ◽  
Potassium Channel ◽  
Binomial Distribution ◽  
Hek293 Cells ◽  
F Cells

Potassium channels play a key role in establishing the cell membrane potential and are expressed ubiquitously. Today, more than 70 mammalian K+ channel genes are known. The diversity of K+ channels is further increased by the fact that different K+ channel family members may assemble to form heterotetramers. We present a method based on fluorescence microscopy to determine the subunit composition of a tetrameric K+ channel. We generated artificial “heteromers” of the K+ channel hKCa3.1 by coexpressing two differently tagged hKCa3.1 constructs containing either an extracellular hemagglutinin (HA) or an intracellular V5 epitope. hKCa3.1 channel subunits were detected in the plasma membrane of MDCK-F cells or HEK293 cells by labeling the extra- and intracellular epitopes with differently colored quantum dots (QDs). As previously shown for the extracellular part of hKCa3.1 channels, its intracellular domain can also bind only one QD label at a time. When both channel subunits were coexpressed, 27.5 ± 1.8% and 24.9 ± 2.1% were homotetramers consisting of HA- and V5-tagged subunits, respectively. 47.6 ± 3.2% of the channels were heteromeric and composed of both subunits. The frequency distribution of HA- and V5-tagged homo- and heteromeric hKCa3.1 channels is reminiscent of the binomial distribution ( a + b)2 = a2 + 2 ab + b2. Along these lines, our findings are consistent with the notion that hKCa3.1 channels are assembled from two homomeric dimers and not randomly from four independent subunits. We anticipate that our technique will be applicable to other heteromeric membrane proteins, too.

Interaction of Chitosan with Cell Membrane Models at the Air−Water Interface

2007 ◽  
Vol 8 (5) ◽  
pp. 1633-1640 ◽  
Author(s):  
Felippe J. Pavinatto ◽  
Adriana Pavinatto ◽  
Luciano Caseli ◽  
David S. dos Santos, ◽  
Thatyane M. Nobre ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Water Interface ◽  
Air Water Interface ◽  
Membrane Models

Localization and interactions of melatonin in dry cholesterol/lecithin mixed reversed micelles used as cell membrane models

2005 ◽  
Vol 38 (4) ◽  
pp. 292-298 ◽  
Author(s):  
David Bongiorno ◽  
Leopoldo Ceraulo ◽  
Mirella Ferrugia ◽  
Felice Filizzola ◽  
Angela Ruggirello ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Reversed Micelles ◽  
Membrane Models

scholarly journals Recent advances in the use of Langmuir monolayers as cell membrane models

2021 ◽  
Vol 46 (1SI) ◽  
pp. 18-29
Author(s):  
Andressa Ribeiro Pereira ◽  
Osvaldo Novais de Oliveira Junior
Keyword(s):  
Cell Membrane ◽  
Review Paper ◽  
Molecular Level ◽  
Langmuir Monolayers ◽  
Pharmaceutical Drugs ◽  
Membrane Composition ◽  
Biologically Relevant ◽  
Membrane Models ◽  
Biologically Relevant Molecules

Understanding the role of biomolecules in cells at the molecular level has been the trade of Prof. Marcio Francisco Colombo and Prof. Jo�o Ruggiero Neto in their carriers, which is why it was found appropriate to address the use of Langmuir monolayers as cell membrane models in this special issue. In the review paper, we elaborate upon the reasons why Langmuir monolayers are good models with the possible control of membrane composition and molecular packing. After describing several experimental methods to characterize the Langmuir monolayers, we discuss selected results from the last five years where monolayers were made to interact with pharmaceutical drugs, emerging pollutants and other biologically-relevant molecules. The challenges to take the field forward are also commented upon.

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