Next day Salmonella spp. detection method based on real-time PCR for meat, dairy and vegetable food products

The aim of the present study was the implementation of molecular techniques in the detection and quantification of allergic substances of peanut in various kinds of food products, e.g., breakfast cereals, chocolates and biscuits that are frequently related to allergies. In some cases, the presence of peanuts can be due to contamination during production and are not declared on the label. A total of 152 samples were collected from supermarkets and were analysed by a Real Time PCR method. The results indicated that 125 samples (83,3%) were found positive in peanut traces but the most important finding is that from the 84 samples that had no allergen declaration for peanuts, 48 (57,1%) of them were found positive. In conclusion, Real Time PCR can be a very important tool for the rapid detection and quantification of food allergens.

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Quantitative real-time PCR assays to detect DNA degradation in soy-based food products

Journal of the Science of Food and Agriculture ◽

10.1002/jsfa.3563 ◽

2009 ◽

Vol 89 (7) ◽

pp. 1137-1144 ◽

Cited By ~ 19

Author(s):

Sarah R Murray ◽

Ruth C Butler ◽

Gail M Timmerman-Vaughan

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Food Products ◽

Dna Degradation ◽

Quantitative Real Time Pcr ◽

Pcr Assays

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A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis) in Food Products

Foods ◽

10.3390/foods9030286 ◽

2020 ◽

Vol 9 (3) ◽

pp. 286 ◽

Cited By ~ 2

Author(s):

Amaya Velasco ◽

Graciela Ramilo-Fernández ◽

Carmen G. Sotelo

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Food Products ◽

Polymerase Chain Reaction Method ◽

Cross Reactivity ◽

Sepia Officinalis ◽

Base Pairs ◽

Mitochondrial Coi ◽

Fresh Frozen ◽

Pcr Method

Cephalopods are very relevant food resources. The common cuttlefish (Sepia officinalis) is highly appreciated by consumers and there is a lack of rapid methods for its authentication in food products. We introduce a new minor groove binding (MGB) TaqMan real-time PCR (Polymerase Chain Reaction) method for the authentication of S. officinalis in food products to amplify a 122 base pairs (bp) fragment of the mitochondrial COI (Cytochrome Oxidase I) region. Reference and commercial samples of S. officinalis showed a threshold cycle (Ct) mean of 14.40, while the rest of the species examined did not amplify, or showed a significantly different Ct (p < 0.001). The calculated efficiency of the system was 101%, and the minimum DNA quantity detected was 10−4 ng. No cross-reactivity was detected with any other species, thus, the designed method differentiates S. officinalis from other species of the genus Sepia and other cephalopod species and works for fresh, frozen, grilled, cooked and canned samples of Sepia spp. The method has proved to be reliable and rapid, and it may prove to be a useful tool for the control of fraud in cuttlefish products.

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Molecular Analyses of Fecal Bacteria and Hydrodynamic Modeling for Microbial Risk Assessment of a Drinking Water Source

Water ◽

10.3390/w12010003 ◽

2019 ◽

Vol 12 (1) ◽

pp. 3

Author(s):

Olga D. Chuquimia ◽

Viktor Bergion ◽

Jessica Guzman-Otazo ◽

Kaisa Sörén ◽

Lars Rosén ◽

...

Keyword(s):

Risk Assessment ◽

Real Time ◽

Water Intake ◽

Real Time Pcr ◽

Hydrodynamic Modeling ◽

Salmonella Spp ◽

Molecular Analyses ◽

Microbial Risk Assessment ◽

Microbial Risk ◽

Campylobacter Spp

Safe water is a global concern, and methods to accurately monitor quality of water are vital. To assess the risks related to bacterial pathogen load in Lake Vomb that provides drinking water to the southern part of Sweden, this study combined molecular analyses of enterobacteria and bacterial pathogens in water using quantitiative real-time PCR with hydrodynamic modeling and quantitative microbial risk assessment (QMRA). A real-time PCR assay to detect enterobacteria was set up by primers targeting ssrA. Between February 2015 and May 2016, presence of ssrA gene copies as well as Campylobacter spp., Salmonella spp., and EHEC O157 DNA was analyzed by real-time PCR at several locations in the catchment of Lake Vomb and its tributaries Björkaån, Borstbäcken, and Torpsbäcken. Björkaån had the highest detected concentrations of the ssrA gene and, according to the results of hydrodynamic modeling, contributed most to the contamination of the water intake in the lake. None of the water samples were positive for genes encoding EHEC O157 and Campylobacter spp., while invA (Salmonella spp.) was present in 11 samples. The QMRA showed that the suggested acceptable risk level (daily probability of infection <2.7 × 10−7) is achieved with a 95% probability, if the Salmonella concentrations in the water intake are below 101 bacteria/100 mL. If a UV-disinfection step is installed, the Salmonella concentration at the water intake should not exceed 106 bacteria/100 mL.

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