Toxicology tailored low density oligonucleotide microarray for the thicklip grey mullets (Chelon labrosus): Biomarker gene transcription profile after caging in a polluted harbour

AbstractCells in simulated microgravity undergo a reversible morphology switch, causing the appearance of two distinct phenotypes. Despite the dramatic splitting into an adherent-fusiform and a floating-spherical population, when looking at the gene-expression phase space, cell transition ends up in a largely invariant gene transcription profile characterized by only mild modifications in the respective Pearson’s correlation coefficients. Functional changes among the different phenotypes emerging in simulated microgravity using random positioning machine are adaptive modifications—as cells promptly recover their native phenotype when placed again into normal gravity—and do not alter the internal gene coherence. However, biophysical constraints are required to drive phenotypic commitment in an appropriate way, compatible with physiological requirements, given that absence of gravity foster cells to oscillate between different attractor states, thus preventing them to acquire a exclusive phenotype. This is a proof-of-concept of the adaptive properties of gene-expression networks supporting very different phenotypes by coordinated ‘profile preserving’ modifications.

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Gene transcription profiling in pollutant exposed mussels (Mytilus spp.) using a new low-density oligonucleotide microarray

Gene ◽

10.1016/j.gene.2006.02.015 ◽

2006 ◽

Vol 376 (1) ◽

pp. 24-36 ◽

Cited By ~ 80

Author(s):

Francesco Dondero ◽

Luciana Piacentini ◽

Francesco Marsano ◽

Mauro Rebelo ◽

Laura Vergani ◽

...

Keyword(s):

Gene Transcription ◽

Oligonucleotide Microarray ◽

Low Density ◽

Transcription Profiling

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BIOFILM – ORGANISATION OF BACTERIA LIFE IN NATURAL ECO SYSTEMS

Archives of Veterinary Medicine ◽

10.46784/e-avm.v1i2.227 ◽

2008 ◽

Vol 1 (2) ◽

pp. 5-15 ◽

Cited By ~ 1

Author(s):

Dubravka Milanov ◽

Ružica Ašanin ◽

Branka Vidić ◽

Dejan Krnjajić ◽

Jelena Petrović

Keyword(s):

Gene Transcription ◽

Intercellular Communication ◽

Biofilm Formation ◽

Transcription Profile ◽

Natural Ecosystems ◽

Microbial Biofilms ◽

Unicellular Organisms ◽

Gene Transcription Profile ◽

Different Characteristics ◽

Chronic Course

In all natural ecosystems, including both humans and animals, bacteria show a tendency to bind on the surface and form a structure known as biofilm. Biofilm formation is a genetically regulated process in the life of bacteria and has several phases demanding intercellular communication. In biofilms bacteria express different characteristics comparing to their free suspended counterparts, due to different gene transcription profile and increased resistance towards antibiotics and disinfectants. Discovery of microbial biofilms has changed our understanding of bacteria, that are not viewed only as unicellular organisms, but more as a multi-cellular community that in some characteristics imitates primitive eukaryotic tissue. In the last decades there is an increasing evidence on infections caused by bacteria that form biofilms, and have a chronic course with possibility of recidives. Conventional methods of killing microbes by antibiotics and biocides is usually ineffective in bacteria organized in biofilms.

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Cell type-dependent gene transcription profile in a three-dimensional human skin tissue model exposed to low doses of ionizing radiation: Implications for medical exposures

Environmental and Molecular Mutagenesis ◽

10.1002/em.21682 ◽

2012 ◽

Vol 53 (4) ◽

pp. 247-259 ◽

Cited By ~ 14

Author(s):

Claere von Neubeck ◽

Harish Shankaran ◽

Norman J. Karin ◽

Paula M. Kauer ◽

William B. Chrisler ◽

...

Keyword(s):

Ionizing Radiation ◽

Human Skin ◽

Gene Transcription ◽

Three Dimensional ◽

Low Doses ◽

Transcription Profile ◽

Cell Type ◽

Tissue Model ◽

Gene Transcription Profile ◽

Human Skin Tissue

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Effects on Gene Transcription Profile and Fatty Acid Composition by Genetic Modification of Mevalonate Diphosphate Decarboxylase MVD/Erg19 in Aspergillus Oryzae

Microorganisms ◽

10.3390/microorganisms7090342 ◽

2019 ◽

Vol 7 (9) ◽

pp. 342

Author(s):

Hu ◽

Huang ◽

Sun ◽

Niu ◽

Xu ◽

...

Keyword(s):

Fatty Acid ◽

Gene Transcription ◽

Aspergillus Oryzae ◽

Acid Content ◽

Ergosterol Biosynthesis ◽

Transcription Profile ◽

Control Strain ◽

Overexpression Strain ◽

Mevalonate Diphosphate Decarboxylase ◽

Gene Transcription Profile

Mevalonate diphosphate decarboxylase MVD/Erg19 is required for ergosterol biosynthesis, growth, sporulation, and stress tolerance in Aspergillus oryzae. In this study, RNA-seq was used to analyze the gene transcription profile in AoErg19 overexpression (OE) and RNAi strains. There were 256 and 74 differentially expressed genes (DEGs) in AoErg19 OE and RNAi strains, respectively, compared with the control strain (CK). The most common DEGs were transport- and metabolism-related genes. Only 22 DEGs were obtained that were regulated in both OE and RNAi strains. The transcriptomic comparison between CK and AoErg19 overexpression strain (CK vs. OE), and between CK and AoErg19 RNAi strain (CK vs. RNAi) revealed that the greatest difference existed in the number of genes belonging to the cytochrome P450 family; 12 were found in CK vs. OE, whereas 1 was found in CK vs. RNAi. The expression patterns of lipid biosynthesis and metabolism related genes were altered in OE and RNAi strains, either by gene induction or suppression. Moreover, the total fatty acid content in the RNAi strain was 12.1% greater than the control strain, but no difference in total acid content was found between the overexpression strain and the control strain. Therefore, this study highlights the gene expression regulation within mevalonate (MVA), ergosterol biosynthesis, and fatty acid biosynthesis pathways.

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