Marine endophytic actinomycetes assisted synthesis of copper nanoparticles (CuNPs): Characterization and antibacterial efficacy against human pathogens

2017 ◽  
Vol 194 ◽  
pp. 176-180 ◽  
Author(s):  
Ubaid Rasool ◽  
S. Hemalatha
Keyword(s):  
Copper Nanoparticles ◽  
Antibacterial Efficacy ◽  
Human Pathogens ◽  
Endophytic Actinomycetes

scholarly journals INVESTIGATION OF THE SYNERGISTIC ANTIBACTERIAL ACTION OF COPPER NANOPARTICLES ON CERTAIN ANTIBIOTICS AGAINST HUMAN PATHOGENS

2018 ◽  
Vol 10 (10) ◽  
pp. 83
Author(s):  
Selvarani Murugan
Keyword(s):  
Antibacterial Activity ◽  
Copper Nanoparticles ◽  
Pathogenic Bacteria ◽  
Color Change ◽  
Quantitative Information ◽  
Inorganic Nanoparticles ◽  
Diffusion Method ◽  
Human Pathogens ◽  
Bacterial Strains ◽  
Agar Disc

Objective: Resistance to antibacterial agents by pathogenic bacteria has emerged in recent years and is a major challenge for the healthcare industry. Copper nanoparticles (CuNPs) are known to be one of the multifunctional inorganic nanoparticles with effective antibacterial activity. Hence the present investigation has been focused on synthesizing and evaluating the bactericidal effect of copper nanoparticles.Methods: CuNPs were synthesized by reducing the aqueous solution of copper sulfate with sodium borohydride. The synthesized particles were characterized by x-ray diffractogram (XRD), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) techniques to analyze size, morphology and quantitative information respectively. The antibacterial activity of CuNPs was examined by agar well diffusion method. Synergistic effect of CuNPs with broad-spectrum antibiotics was determined by the agar disc diffusion method.Results: Color change of reaction mixture from blue to dark brown indicated the formation of CuNPs. SEM image clearly demonstrated that the synthesized particles were spherical in shape and its size was found to be 17.85 nm. EDS report confirmed the presence of elemental copper in the resultant nanoparticles and its accounts for major proportion (96%) of the mass of nanoparticles. Bacterial effect of CuNPs revealed that Pseudomonas aeruginosa showed the highest antibacterial sensitivity (16.00±1.63 mm), whereas least susceptibility (9.67±0.47 mm) was noticed against Staphylococcus aureus. An enhanced antibacterial activity of commercial antibiotics was also noticed when it combined with CuNPS. A minimum zone of inhibition was increased from 0.67±0.47 mm to 10.66±0.24 mm when the nanoparticles and antibiotics were given together.Conclusion: It was observed that copper nanoparticles exhibited profound activity against all the tested bacterial strains which shows that CuNPs may serve as a better option for use in medicine in the future.

Bacterial endophytes from Artemisia nilagirica (Clarke) Pamp., with antibacterial efficacy against human pathogens

2019 ◽  
Vol 135 ◽  
pp. 103624 ◽  
Author(s):  
A. Ashitha ◽  
S.J. Midhun ◽  
M.A. Sunil ◽  
T.U. Nithin ◽  
E.K. Radhakrishnan ◽  
...  
Keyword(s):  
Antibacterial Efficacy ◽  
Bacterial Endophytes ◽  
Human Pathogens ◽  
Artemisia Nilagirica

scholarly journals New approach for antimicrobial activity and bio-control of various pathogens by biosynthesized copper nanoparticles using endophytic actinomycetes

2018 ◽  
Vol 11 (3) ◽  
pp. 262-270 ◽  
Author(s):  
Saad EL-Din Hassan ◽  
Salem S. Salem ◽  
Amr Fouda ◽  
Mohamed A. Awad ◽  
Mamdouh S. El-Gamal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Copper Nanoparticles ◽  
New Approach ◽  
Endophytic Actinomycetes

scholarly journals Isolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens

2016 ◽  
Vol 8 (1) ◽  
pp. 416-422 ◽  
Author(s):  
Preeti Saini ◽  
Madhurama Gangwar ◽  
Anu Kalia ◽  
Narinder Singh ◽  
Deepti Narang
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Chitinase Activity ◽  
Syzygium Cumini ◽  
Human Pathogens ◽  
Endophytic Actinomycetes ◽  
Antistaphylococcal Activity ◽  
Leaf Tissues ◽  
Drug Resistant Strains ◽  
Promising Source

Isolation of endophytic actinomycetes is an important step to screen antimicrobial compounds to curb the threat of drug-resistant strains of human pathogens. Out of the 50 endophytic actinomycetes obtained from surface sterilized root, stem and leaf tissues of Syzygium cumini, 50 isolates (30%) exhibited antimicrobial activity. Antistaphylococcal activity was displayed by most of the isolates, with maximum percent inhibition by J-10 (Mean of Inhibition Factor=12.12 mm2). A total of 8 isolates (4 each) were able to hydrolyse protein (proteinase activity) and solubilize chitin (chitinase activity). Results of thin layer chromatography confirm the production of chloramphenicol family |antibiotic by the isolate J-5. This is the first report providing an insight into untapped endophytic actinomycete milieu of Syzygium cumini yet to be explored which might be a promising source for novel antimicrobial agents.

Plasminogen Activation by Invasive Human Pathogens

1997 ◽  
Vol 77 (01) ◽  
pp. 001-010 ◽  
Author(s):  
Michael D P Boyle ◽  
Richard Lottenberg
Keyword(s):  
Plasminogen Activators ◽  
Plasminogen Activation ◽  
Potential Importance ◽  
Human Pathogens

SummaryIn this review the interaction between invasive human pathogens expressing plasmin(ogen) receptors and/or producing plasminogen activators with the human plasmin(ogen) system is described. Evidence is presented for multiple mechanisms by which human pathogens can acquire a surface bound form of plasmin that cannot be regulated by host serpins. The potential importance of these pathways in providing the organisms with the ability to cross tissue barriers is discussed.

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