scholarly journals Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes

2021 ◽  
pp. 105131
Author(s):  
Marco A. Brovedan ◽  
Patricia M. Marchiaro ◽  
María S. Díaz ◽  
Diego Faccone ◽  
Alejandra Corso ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Group Species

scholarly journals Pseudomonas putida group species serve as reservoirs of conjugative plasmids disseminating Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes

2020 ◽  
Author(s):  
Marco A. Brovedan ◽  
Patricia M. Marchiaro ◽  
María S. Díaz ◽  
Diego Faccone ◽  
Alejandra Corso ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Phylogenetic Analyses ◽  
Sequencing Analysis ◽  
Class 1 Integrons ◽  
Complete Transposition ◽  
Carbapenem Resistant ◽  
Wide Range ◽  
Class 1 ◽  
Well Differentiated ◽  
Group Species

ABSTRACTThe Pseudomonas putida group (P. putida G) is composed of at least 21 species associated to a wide range of environments, including the clinical setting. Here, we characterized 13 carbapenem-resistant P. putida G clinical isolates carrying blaVIM-2 from different hospitals of Argentina. Multilocus sequencing (MLSA) and phylogenetic analyses based on the 16S rDNA, gyrB and rpoD sequences comparison allowed us to assign them to 7 well-differentiated species. Sequencing analysis revealed that blaVIM-2 genes were carried in these isolates by three different class 1 integrons (In41, In899 and In528) embedded into Tn402-like transposons. Those harboring In41 and In899 were designated Tn6335 and Tn6336, respectively, with the former found among 10 isolates. Both encompassed complete transposition modules and inverted repeats boundaries characteristic of the Tn5053/Tn402 family, whereas the third, bearing In528, exhibited a defective tni module. Tn6335 and Tn6336 were located in conjugative pLD209-type plasmids in P. asiatica, P. juntendi, P. putida G/II, and P. putida G/V isolates, and could be mobilized to Escherichia coli and P. aeruginosa indicating a relevant mechanism of blaVIM-2 dissemination. In other P. asiatica and P. putida G/II isolates, Tn6335 was found inserted into the Tn21 subgroup transposons-res region, indicating capability for intragenomic mobilization and further dissemination associated to Tn3 family transposons. The Tn402-like defective element was also found inserted into the res region of another Tn3 family transposon in a P. monteilii isolate, but in an atypical orientation. Overall findings shed light on the mechanisms by which resistance genes move through environmental and opportunist Pseudomonas species.

Antimicrobial Resistance and Presence of Class 1 Integrons Among Different Serotypes of Salmonella spp. Recovered From Children with Diarrhea in Tehran, Iran

2020 ◽  
Vol 20 (2) ◽  
pp. 160-166
Author(s):  
Seyedeh Hanieh Eshaghi Zadeh ◽  
Hossein Fahimi ◽  
Fatemeh Fardsanei ◽  
Mohammad Mehdi Soltan Dallal
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Class 1 Integron ◽  
Class 1 Integrons ◽  
Food Borne ◽  
Class 1 ◽  
Resistance Patterns ◽  
C 30

Background: Salmonellosis is a major food-borne disease worldwide. The increasing prevalence of antimicrobial resistance among food-borne pathogens such as Salmonella spp. is concerning. Objective: The main objective of this study is to identify class 1 integron genes and to determine antibiotic resistance patterns among Salmonella isolates from children with diarrhea. Methods: A total of 30 Salmonella isolates were recovered from children with diarrhea. The isolates were characterized for antimicrobial susceptibility and screened for the presence of class 1 integron genes (i.e. intI1, sulI1, and qacEΔ1). Results: The most prevalent serotype was Enteritidis 36.7%, followed by Paratyphi C (30%), and Typhimurium (16.7%). The highest rates of antibiotic resistance were obtained for nalidixic acid (53.3%), followed by streptomycin (40%), and tetracycline (36.7%). Regarding class 1 integrons, 36.7%, 26.7%, and 33.3% of the isolates carried intI1, SulI, and qacEΔ1, respectively, most of which (81.8%) were multidrug-resistant (MDR). Statistical analysis revealed that the presence of class 1 integron was significantly associated with resistance to streptomycin and tetracycline (p = 0.042). However, there was no association between class 1 integron and other antibiotics used in this study (p > 0.05). Conclusion: The high frequency of integron class 1 gene in MDR Salmonella strains indicates that these mobile genetic elements are versatile among different Salmonella serotypes, and associated with reduced susceptibility to many antimicrobials.

Class 1 integrons as predominant carriers in Escherichia coli isolates from waterfowls in Hainan, China

2019 ◽  
Vol 183 ◽  
pp. 109514 ◽  
Author(s):  
Shaqiu Zhang ◽  
Hong Yang ◽  
Mujeeb Ur Rehman ◽  
Kema Yang ◽  
Mengyi Dong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Class 1 Integrons ◽  
Class 1

Antibiotic selective pressure for the maintenance of antibiotic resistant genes in coliform bacteria isolated from the aquatic environment

2003 ◽  
Vol 47 (3) ◽  
pp. 249-253 ◽  
Author(s):  
J.C. Park ◽  
J.C. Lee ◽  
J.Y. Oh ◽  
Y.W. Jeong ◽  
J.W. Cho ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Selective Pressure ◽  
Functional Class ◽  
Coliform Bacteria ◽  
Antibiotic Resistant ◽  
Gene Cassettes ◽  
Class 1 Integrons ◽  
Selective Pressures ◽  
Class 1 ◽  
Inti1 Gene

Coliform bacteria isolated from the aquatic environment were investigated for antibiotic susceptibility and detailed structures of class 1 integrons. A high proportion of isolates were found to be resistant to sulfamethoxazole, aminoglycosides, and β-lactams. The 750 (53.6%) isolates were resistant to one or more of the antibiotics tested out of 1,400 coliform bacteria. Based on the MIC of antibiotics and antibiogram, 150 isolates were selected and further studied for class 1 integrons. The intI1 gene was found in 36 (24.0%) of the 150 isolates. Twelve isolates carried the gene cassettes responsible for antibiotic resistance, while no gene cassettes were found in 24 isolates. Seven different genes, dfrA5, dfrA7, dfrA12, dfrA17, aaA2, aaA5, and aad(3’), were detected in gene cassettes. The dfrA and aad genes located on class 1 integrons were responsible for resistance to trimethoprim and aminoglycosides. The remaining 24 coliform bacteria had the incomplete or non-functional class 1 integrons. These results indicated that antibiotic selective pressures may play an important role to maintain gene cassettes of class 1 integrons and in the absence of sustained antibiotic pressures, such as the aquatic environment, coliform bacteria may carry empty or non-functional class 1 integrons.

scholarly journals Integron Expression in Multidrug-Resistant Escherichia coli Isolated from House Flies within the Hospital

2018 ◽  
Vol 16 (5) ◽  
pp. 319-327
Author(s):  
Atchariya YOSBOONRUANG ◽  
Anong KIDDEE ◽  
Chatsuda BOONDUANG ◽  
Phannarai PIBALPAKDEE
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Hospital Environment ◽  
Sequencing Analysis ◽  
Class 1 Integron ◽  
House Flies ◽  
E Coli ◽  
Class 1 Integrons ◽  
Class 1

Escherichia coli is a serious cause of a variety of hospital-acquired infections and commonly contributes to the environment by house flies. Integrons, particularly class 1 integrons, are the genetic elements that play an important role in the horizontal transfer of antimicrobial resistance mechanism. This mechanism is commonly found in Enterobacteriaceae, especially E. coli. In this study, we aim to investigate the occurrence and antimicrobial resistance patterns of E. coli isolated from the house flies in Phayao hospital and to determine the gene expression of class 1 integrons in those isolates of E. coli. Totally, 70 isolates of E. coli were isolated from 60 house flies collected from the hospital. Fifty-seven of the isolates (81.43 %) were multidrug resistance (MDR) and highly resistant to b-lactams, tetracyclines, and sulfonamides. Of 57 isolates of MDR-E. coli, 20 isolates (35 %) were found to carry class 1 integron genes. Fifteen patterns of antimicrobial resistance occurred in the isolates of integron-positive E. coli. Most integron-positive E. coli isolates were resistant to 7 antimicrobials. Two isolates of these bacteria (10 %) were able to resist 13 out of 14 tested antimicrobials. Using PCR and sequencing analysis, an investigation showed that dfrA17-aadA5, dfrA12-aadA2 gene cassette was the most prevalent cassette (n = 10; 50 %) among the integron-positive E. coli isolates. Our results indicated that the presences of multidrug resistance and class 1 integrons were common in E. coli isolated from the houseflies in hospital. Therefore, screening for integron-positive E. coli from the hospital environment might be necessary for prevention of nosocomial infections.

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