Detection of cord blood hepcidin levels as a biomarker for early-onset neonatal sepsis

Use of empirical antibiotics in neonates with risk factors of early-onset neonatal sepsis (EOS) is a common practice. A laboratory parameter is needed to help in the accurate diagnosis of EOS to avoid unnecessary use of antibiotics. The aim of this prospective observational cohort study was to compare the statistical validity of cord blood interleukin-6 (IL-6) with conventional sepsis screening as an early diagnostic marker for EOS. Eighty-seven neonates with antenatal risk factors for sepsis were followed up for 72 h for the development of EOS. Cord blood was collected for measurement of IL-6 concentrations. Blood culture and conventional sepsis screening (total leukocyte count, absolute neutrophil count, C-reactive protein and micro-erythrocyte sedimentation rate) were sent for analysis soon after delivery. The study group comprised of symptomatic neonates with positive blood culture (n=36). An equal number of gestational-age matched asymptomatic neonates without risk factor of sepsis served as controls. Statistical validity of IL-6 was compared with sepsis screening parameters as the diagnostic marker for EOS. Gram negative organisms were the predominant cause of EOS. The most commonly isolated organism was <em>Acinetobacter baumanii</em>. The sensitivity and specificity of IL-6 with a cut-off value of 40.5 pg/mL and area under curve of 0.959 were 92.3 and 90.48%, respectively. In contrast, the sensitivity and specificity of different parameters of sepsis screening ranged from 37.5-68.75% and 47.95-57.35%, respectively. In conclusion, cord blood IL-6 can be used as a highly sensitive and specific early diagnostic marker of EOS at a cut-off concentration of 40.5 pg/mL.

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EVALUATION OF CORD BLOOD CULTURE AS AN EARLY PREDICTOR FOR THE DETECTION OF EARLY ONSET NEONATAL SEPSIS IN TERTIARY CENTRE OF CENTRAL INDIA.

10.36106/gjra/2510225 ◽

2021 ◽

pp. 84-86

Author(s):

Gagandeep Shukla ◽

Urvashi Channa

Keyword(s):

Blood Culture ◽

Cord Blood ◽

Neonatal Sepsis ◽

Early Onset ◽

Sequential Sampling ◽

Venous Blood ◽

Central India ◽

Neonatal Morbidity ◽

Peripheral Venous Blood ◽

Tertiary Centre

BACKGROUND : Neonatal sepsis is one of the major causes of neonatal morbidity and mortality. Peripheral venous blood culture and sensitivity is gold standard for the diagnosis of neonatal sepsis. Low sensitivity of blood culture in newborn is due to small volume of blood sample collected from neonates & antibiotics given before sampling. AIM : To evaluate the utility & compare the Umblical cord blood culture(UCBC) with Peripheral venous blood culture(PVBC) for detection of Early Onset Neonatal Sepsis(EONS) METHOD : 100 inborn neonates with two or more risk factors for EONS, chosen by sequential sampling method were included in this prospective analytical study. Blood samples were collected from umbilical cord and peripheral vein for culture. Sepsis screen was done to corroborate the diagnosis of neonatal sepsis. RESULT : Out of 100 neonates, 21 belongs to sepsis; 14 to probable sepsis; 65 to no sepsis. UCBC had Sensitivity-65.71% , Specicity-93.84%, PPV-85.18% , NPV-83.56% & PVBC had Sensitivity-60% , Specicity-95.38% , PPV-87.5% , NPV-81.57%. CONCLUSION : UCBC is simple and convenient method for the diagnosis of EONS compared to PVBC. Organisms grown are comparable to PVBC sample.

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Early-onset neonatal sepsis: cord blood cytokine levels at diagnosis and during treatment

Jornal de Pediatria ◽

10.2223/jped.2043 ◽

2010 ◽

Vol 0 (0) ◽

Cited By ~ 5

Author(s):

Dulcimar P. Campos ◽

Marcos V. Silva ◽

Juliana R. Machado ◽

Lúcio R. Castellano ◽

Virmondes Rodrigues ◽

...

Keyword(s):

Cord Blood ◽

Neonatal Sepsis ◽

Early Onset ◽

Cytokine Levels

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Umbilical Cord Blood for the Screening of Early Onset Neonatal Sepsis among Those at Risk of Infection

Scholars Journal of Applied Medical Sciences ◽

10.36347/sjams.2021.v09i03.032 ◽

2021 ◽

Vol 9 (3) ◽

pp. 452-461

Author(s):

Dr. Mohammad Shakhawat Alam ◽

Prof. M.A. Mannan ◽

Dr. Sanjoy Kumer Dey ◽

Dr. A Z M Raihanur Rahman ◽

Prof. Mohammod Shahidullah

Keyword(s):

At Risk ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Neonatal Sepsis ◽

Early Onset ◽

Risk Of Infection

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Umbilical cord blood culture versus peripheral venous blood culture in early onset neonatal sepsis

International Journal of Contemporary Pediatrics ◽

10.18203/2349-3291.ijcp20164302 ◽

2017 ◽

Author(s):

Sanjay Mandot ◽

Jeetendra Gandhi

Keyword(s):

Blood Culture ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Neonatal Sepsis ◽

Early Onset ◽

Venous Blood ◽

Peripheral Venous Blood

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Presepsin as a predictor of early onset neonatal sepsis in the umbilical cord blood of premature infants with premature rupture of membranes

Pediatrics International ◽

10.1111/ped.13541 ◽

2018 ◽

Vol 60 (5) ◽

pp. 428-432 ◽

Cited By ~ 3

Author(s):

Wael Seliem ◽

Amira M. Sultan

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Premature Infants ◽

Umbilical Cord Blood ◽

Neonatal Sepsis ◽

Early Onset ◽

Premature Rupture Of Membranes ◽

Premature Rupture

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Umbilical Cord Blood Culture in Diagnosis of Early Onset Neonatal Sepsis

The Indian Journal of Pediatrics ◽

10.1007/s12098-020-03345-5 ◽

2020 ◽

Vol 87 (10) ◽

pp. 793-797 ◽

Cited By ~ 1

Author(s):

Ramraj Meena ◽

Kailash Kumar Meena ◽

Vivek Athwani ◽

Sunil Gothwal ◽

Ghan Shyam Bairwa ◽

...

Keyword(s):

Blood Culture ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Neonatal Sepsis ◽

Early Onset

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Assessment of oxidative damage and enzymatic antioxidant system activity on the umbilical cord blood and saliva from preterm newborns with risk factors for early-onset neonatal sepsis

Revista da Associação Médica Brasileira ◽

10.1590/1806-9282.64.10.888 ◽

2018 ◽

Vol 64 (10) ◽

pp. 888-895 ◽

Cited By ~ 1

Author(s):

Fábio Gonçalves Coutinho ◽

Edna Maria de Albuquerque Diniz ◽

Ingrid Kandler ◽

Marco Antônio Cianciarullo ◽

Natália Rodrigues dos Santos

Keyword(s):

Risk Factors ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Neonatal Sepsis ◽

Early Onset ◽

Umbilical Vein ◽

Statistical Significance ◽

Cross Sectional ◽

Significant Difference

SUMMARY BACKGROUND: To determine the concentration of the Lipid Peroxidation Marker: Malondialdehyde (MDA), and Antioxidant Markers: Superoxide Dismutase (SOD), Glutathione Peroxidase (GPX), Catalase (CAL) in umbilical cord blood and in unstimulated saliva in the first 24 and 48 hours of life in the PTNB of mothers with and without risk factors for early-onset neonatal sepsis. METHODS: Cross-sectional study with the signing of informed consent by the pregnant women and application of a standard questionnaire classifying the PTNB in Group 1 or 2. RESULTS: Twenty-one PTNB were studied. Regarding gender, birth weight, need for oxygen, use of phototherapy, diagnosis of assumed sepsis, presence of fetal distress, number of pregnancies, type of delivery, use of corticosteroids, premature rupture of membranes, maternal fever, chorioamnionitis, APGAR at the 5th and 10th minute of life. Statistical analysis was performed with the Mann-Whitney test (p = 0.019) on the GPX variable of umbilical cord blood in the group of mothers with risk factors for early-onset neonatal sepsis. There was no statistical difference in the MDA, SOD, and CAT variables of the group with risk factors and in any variable of the group without risk factors. CONCLUSION: There was an increase of the GPX concentration in the blood from the umbilical vein in the group with risk factors for early-onset neonatal sepsis. There was no statistical significance in the comparison of saliva and umbilical cord blood. There was no statistically significant difference in MDA, SOD, CAT.

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