scholarly journals A one-step platform for screening high-efficient and minimal off-target CRISPR/Cas13 crRNAs to eradicate SARS-CoV-2 virus for treatment of COVID-19 patients

2022 ◽  
pp. 110754
Author(s):  
Pu-Yu Li ◽  
San-Qiang Li ◽  
She-Gan Gao ◽  
Dao-Yin Dong
Keyword(s):  
High Efficient ◽  
One Step

scholarly journals One-Step Bark-Like Imitated Polypropylene (PP)/Polycarbonate (PC) Nanofibrous Meltblown Membrane for Efficient Particulate Matter Removal

Polymers ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1307 ◽  
Author(s):  
Ting-Ting Li ◽  
Xixi Cen ◽  
Hai-Tao Ren ◽  
Fei Sun ◽  
Qi Lin ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Fiber Diameter ◽  
Average Fiber Diameter ◽  
Efficient Removal ◽  
Filtration Performance ◽  
Innovative Method ◽  
High Efficient ◽  
One Step ◽  
The Cost ◽  
Pm Removal

A bark-like imitated polypr opylene (PP)/polycarbonate (PC) nanofibrous membrane was constructed by one-step meltblown technique for efficient particulate matter (PM) removal. The effects of PC content (0%, 1%, 3%, 5%, and 7%) on membrane thermal stability, microscopic characteristics, filtration performance, hydrophilicity, and water vapor transmission were investigated. The results demonstrated that using facile design of incompatibility and viscosity difference between PC and PP polymers decreases average fiber diameter, creating a bark-like groove appearance and increasing surface potential, making a new PP/PC membrane with high filtration performance. The resultant PP/PC membrane had finer average fiber diameter of 0.63 μm, which was nearly 89.41% lower than PP membranes (5.95 μm), and its quality factor (0.036 Pa−1) was nearly 2.12 times than that of PP membranes (0.017 Pa−1) with the die hole diameter of 0.5 mm. This fabrication technique of a special meltblown filter membrane saves the cost of die retrofitting and post-processing, which provides an innovative method for particulate efficient removal of high efficient filters.

Tunable ternary (P, S, N)-doped graphene as an efficient electrocatalyst for oxygen reduction reaction in an alkaline medium

RSC Advances ◽  
2015 ◽  
Vol 5 (105) ◽  
pp. 86746-86753 ◽  
Author(s):  
Yishu Wang ◽  
Bowen Zhang ◽  
Minghui Xu ◽  
Xingquan He
Keyword(s):  
Oxygen Reduction Reaction ◽  
Oxygen Reduction ◽  
Low Cost ◽  
Reduction Reaction ◽  
Efficient Catalyst ◽  
Pyrolysis Method ◽  
High Efficient ◽  
Doped Graphene ◽  
One Step ◽  
Nitrogen Phosphorus

A novel nitrogen, phosphorus and sulfur ternary-doped graphene (PSNG) was synthesized by a one-step pyrolysis method, which was used as a low-cost and high-efficient catalyst for the oxygen reduction reaction.

One-step-MACE nano/microstructures for high-efficient large-size multicrystalline Si solar cells

2015 ◽  
Vol 143 ◽  
pp. 302-310 ◽  
Author(s):  
Z.G. Huang ◽  
X.X. Lin ◽  
Y. Zeng ◽  
S.H. Zhong ◽  
X.M. Song ◽  
...  
Keyword(s):  
Solar Cells ◽  
Large Size ◽  
Si Solar Cells ◽  
High Efficient ◽  
One Step

Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin

2004 ◽  
Vol 37 (2) ◽  
pp. 361-367 ◽  
Author(s):  
Chenyun Guo ◽  
Zhuoyu Li ◽  
Yawei Shi ◽  
Mingqun Xu ◽  
John G. Wise ◽  
...  
Keyword(s):  
Fusion Expression ◽  
High Efficient ◽  
One Step

scholarly journals A one-step tRNA-CRISPR system for genome-wide genetic interaction mapping in mammalian cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yulei Zhao ◽  
Kathrin Tyrishkin ◽  
Calvin Sjaarda ◽  
Prem Khanal ◽  
Jeff Stafford ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Drug Targets ◽  
Genetic Interaction ◽  
Genetic Interactions ◽  
Cancer Treatments ◽  
Genome Wide ◽  
A Genome ◽  
High Efficient ◽  
One Step ◽  
Screening Accuracy

Abstract Mapping genetic interactions in mammalian cells is limited due to technical obstacles. Here we describe a method called TCGI (tRNA-CRISPR for genetic interactions) to generate a high-efficient, barcode-free and scalable pairwise CRISPR libraries in mammalian cells for identifying genetic interactions. We have generated a genome- wide library to identify genes genetically interacting with TAZ in cell viability regulation. Validation of candidate synergistic genes reveals the screening accuracy of 85% and TAZ-MCL1 is characterized as combinational drug targets for non-small cell lung cancer treatments. TCGI has dramatically improved the current methods for mapping genetic interactions and screening drug targets for combinational therapies.

Purification of phloridzin from Lithocarpus polystachyus Rehd leaves using polymeric adsorbents functionalized with glucosamine and β-cyclodextrin

2014 ◽  
Vol 68 (11) ◽  
Author(s):  
Qian-Wen Wang ◽  
Jia-Jia Liu ◽  
Xiong Liu ◽  
Hong-Wei Zha ◽  
Na Ren
Keyword(s):  
Fourier Transform ◽  
Adsorption Capacity ◽  
Chromatographic Separation ◽  
Adsorption Properties ◽  
Purification Method ◽  
Freundlich Model ◽  
High Efficient ◽  
One Step ◽  
The Fourier Transform ◽  
Column Chromatographic

AbstractA high-efficient purification method of flavonoids from Lithocarpus polystachyus Rehd leaves is reported. Two adsorbents functionalized with glucosamine (GA) and β-cyclodextrin (β-CD) were synthesized and characterized by the Fourier transform infrared (FTIR) spectra. Also, adsorption properties and purification effect of flavonoids on different adsorbents were investigated. Two typical commercial adsorbents, AB-8 and D101, were employed as the reference materials. The adsorption capacity was enhanced remarkably by the introduction of GA and β-CD groups to the adsorbents. Moreover, the isotherms were well fitted by the Freundlich model. After a one-step column chromatographic separation, the purity of phloridzin increased significantly from 14.65 % to 79.48 % for PS-CD, to 66.56 % for PS-GA, 44.68 % for D101, and to 52.84 % for AB-8. This study provides a novel alternative matrix for the purification of phloridzin from Lithocarpus polystachyus Rehd leaves extracts.

Facile synthesis of novel NH2-MIL-53(Fe)/AgSCN heterojunction composites as high-efficient photocatalyst for ciprofloxacin degradation and H2 production under visible-light irradiation

2021 ◽  
Author(s):  
Jungang Yi ◽  
Xianghui Wu ◽  
Huadong Wu ◽  
Kun Wu ◽  
Jia Guo ◽  
...  
Keyword(s):  
Visible Light ◽  
Visible Light Irradiation ◽  
Chemical Precipitation ◽  
H2 Production ◽  
Energy Crisis ◽  
Precipitation Method ◽  
Facile Synthesis ◽  
Composite Photocatalyst ◽  
High Efficient ◽  
One Step

Facing the increasingly serious problem of environmental pollution and energy crisis, a novel NH2-MIL-53(Fe)/AgSCN (NMFA) composite photocatalyst was successfully prepared through a one-step chemical precipitation method in the present work....

scholarly journals One-step electrodeposition of platinum nanoflowers and their high efficient catalytic activity for methanol electro-oxidation

2010 ◽  
Vol 12 (7) ◽  
pp. 882-885 ◽  
Author(s):  
Hongmei Zhang ◽  
Weiqiang Zhou ◽  
Yukou Du ◽  
Ping Yang ◽  
Chuanyi Wang
Keyword(s):  
Catalytic Activity ◽  
High Efficient ◽  
One Step ◽  
Electro Oxidation
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