Immunologically relevant strain polymorphism in the Amastigote Surface Protein 2 of Trypanosoma cruzi

Metacyclic trypomastigotes ol the CL strain of Trypanosoma cruzi obtained from triatomid vectors and from axenic cultures were comparatively analysed as to their antigen make-up and immunogenic characteristics. They were found to be similar by the various parameters examined. Thus, sera of mice immunized with either one of the two metacyclic types precipitated a 82Kd surface protein from 131I-labeled culture metacyclics. Sera of mice protected against acute T. cruzi infection by immunization with killed culture metacyclics of a different strain (G) recognized, by immunoblotting, a 77Kd protein in both types of CL strain metacyclics. A monoclonal antibody raised against G strain metacyclics, and specific for metacyclic stages of this strain, reacted with both CL strain metacyclic types. Both metacyclic forms were similarly Iysed by various anti-T. cruzi sera, in a complement-mediated reaction.

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Protective Immunity Against Trypanosoma cruzi Infection in a Highly Susceptible Mouse Strain After Vaccination with Genes Encoding the Amastigote Surface Protein-2 and Trans-Sialidase

Human Gene Therapy ◽

10.1089/hum.2004.15.878 ◽

2004 ◽

Vol 15 (9) ◽

pp. 878-886 ◽

Cited By ~ 51

Author(s):

José Ronnie Vasconcelos ◽

Meire I. Hiyane ◽

Cláudio R.F. Marinho ◽

Carla Claser ◽

Alexandre M.V. Machado ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Mouse Strain ◽

Protective Immunity ◽

Surface Protein ◽

Susceptible Mouse ◽

Genes Encoding ◽

Cruzi Infection

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cDNA cloning and partial characterization of amastigote specific surface protein from Trypanosoma cruzi

Infection Genetics and Evolution ◽

10.1016/j.meegid.2009.05.016 ◽

2009 ◽

Vol 9 (6) ◽

pp. 1083-1091 ◽

Cited By ~ 5

Author(s):

Marybell Olivas-Rubio ◽

Salvador Hernández-Martínez ◽

Patricia Talamás-Rohana ◽

Victor Tsutsumi ◽

Pedro A. Reyes-López ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Specific Surface ◽

Cdna Cloning ◽

Surface Protein ◽

Partial Characterization

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The SA85-1.1 Protein of the Trypanosoma cruzi trans-Sialidase Superfamily Is a Dominant T-Cell Antigen

Infection and Immunity ◽

10.1128/iai.68.6.3574-3580.2000 ◽

2000 ◽

Vol 68 (6) ◽

pp. 3574-3580 ◽

Cited By ~ 9

Author(s):

Amanda E. Millar ◽

Stuart J. Kahn

Keyword(s):

T Cell ◽

Trypanosoma Cruzi ◽

Surface Protein ◽

T Cell Response ◽

Cd4 Cells ◽

Protective Response ◽

Mhc Ii ◽

Histocompatibility Complex ◽

Class Ii Mhc ◽

Ifn Γ

ABSTRACT Trypanosoma cruzi currently infects 18 million people, and 30% of those infected develop a chronic inflammatory process that causes significant morbidity or mortality. The major histocompatibility complex class II (MHC-II)-restricted T-cell response is critical to the control of the infection and to the ensuing inflammatory pathology. The specific epitopes or major antigens of this response have not been identified. The parasite simultaneously expresses variant members of the trans-sialidase superfamily. To begin to analyze the MHC-II response to these variant proteins, the response to a single surface protein, SA85-1.1, was initiated. These studies have demonstrated that a biased gamma interferon (IFN-γ) response to the SA85-1.1 protein develops during T. cruzi infection. In addition, adoptive transfer of a CD4 clone that recognizes an SA85-1.1 epitope, named epitope 1, and immunization with a peptide encoding epitope 1 were protective and suggested that epitope 1 may be immunodominant. In this report IFN-γ intracellular staining demonstrated that splenocytes from acutely and chronically infected mice, incubated with SA85-1.1 protein or peptides that encode epitope 1, result in IFN-γ synthesis by 4 to 6% of the splenic CD4 cells. These data indicate that during T. cruzi infection epitope 1 is a major epitope and that 4 to 6% of the CD4 cells are stimulated by a single trans-sialidase superfamily epitope and suggest that a combination of trans-sialidase superfamily proteins combines to stimulate a majority of CD4 cells. These data suggest that during T. cruzi infection the CD4 response to thetrans-sialidase superfamily is critical to the protective response and to the ensuing chronic inflammatory pathology.

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CD8+-T-Cell-Dependent Control of Trypanosoma cruzi Infection in a Highly Susceptible Mouse Strain after Immunization with Recombinant Proteins Based on Amastigote Surface Protein 2

Infection and Immunity ◽

10.1128/iai.73.9.6017-6025.2005 ◽

2005 ◽

Vol 73 (9) ◽

pp. 6017-6025 ◽

Cited By ~ 44

Author(s):

Adriano F. S. Araújo ◽

Bruna C. G. de Alencar ◽

José Ronnie C. Vasconcelos ◽

Meire I. Hiyane ◽

Cláudio R. F. Marinho ◽

...

Keyword(s):

Amino Acids ◽

T Cells ◽

T Cell ◽

Recombinant Protein ◽

Trypanosoma Cruzi ◽

Recombinant Proteins ◽

Protective Immunity ◽

Protective Efficacy ◽

Surface Protein ◽

Cpg Oligodeoxynucleotide

ABSTRACT We previously described that DNA vaccination with the gene encoding amastigote surface protein 2 (ASP-2) protects approximately 65% of highly susceptible A/Sn mice against the lethal Trypanosoma cruzi infection. Here, we explored the possibility that bacterial recombinant proteins of ASP-2 could be used to improve the efficacy of vaccinations. Initially, we compared the protective efficacy of vaccination regimens using either a plasmid DNA, a recombinant protein, or both sequentially (DNA priming and protein boosting). Survival after the challenge was not statistically different among the three mouse groups and ranged from 53.5 to 75%. The fact that immunization with a recombinant protein alone induced protective immunity revealed the possibility that this strategy could be pursued for vaccination. We investigated this possibility by using six different recombinant proteins representing distinct portions of ASP-2. The vaccination of mice with glutathione S-transferase fusion proteins representing amino acids 261 to 500 or 261 to 380 of ASP-2 in the presence of the adjuvants alum and CpG oligodeoxynucleotide 1826 provided remarkable immunity, consistently protecting 100% of the A/Sn mice. Immunity was completely reversed by the in vivo depletion of CD8+ T cells, but not CD4+ T cells, and was associated with the presence of CD8+ T cells specific for an epitope located between amino acids 320 and 327 of ASP-2. We concluded that a relatively simple formulation consisting of a recombinant protein with a selected portion of ASP-2, alum, and CpG oligodeoxynucleotide 1826 might be used to cross-prime strong CD8+-T-cell-dependent protective immunity against T. cruzi infection.

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Molecular cloning of the gene encoding the 83 kDa amastigote surface protein and its identification as a member of the Trypanosoma cruzi sialidase superfamily1Note: Nucleotide sequence data reported in this paper is available in the GenBank™ database under the accession number U77951.1

Molecular and Biochemical Parasitology ◽

10.1016/s0166-6851(97)00088-1 ◽

1997 ◽

Vol 88 (1-2) ◽

pp. 137-149 ◽

Cited By ~ 29

Author(s):

Hoi P Low ◽

Rick L Tarleton

Keyword(s):

Nucleotide Sequence ◽

Trypanosoma Cruzi ◽

Molecular Cloning ◽

Sequence Data ◽

Surface Protein ◽

Accession Number ◽

Gene Encoding ◽

Nucleotide Sequence Data

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Analysis of mRNA processing at whole transcriptome level, transcriptomic profile and genome sequence refinement of Trypanosoma cruzi

Scientific Reports ◽

10.1038/s41598-019-53924-6 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Francisco Callejas-Hernández ◽

Ángel Gutierrez-Nogues ◽

Alberto Rastrojo ◽

Núria Gironès ◽

Manuel Fresno

Keyword(s):

Trypanosoma Cruzi ◽

Genomic Sequence ◽

Surface Protein ◽

Mrna Processing ◽

The Other ◽

Spliced Leader ◽

Rnaseq Data ◽

Whole Transcriptome ◽

First Time ◽

Transcriptome Level

AbstractThe genomic sequence of Trypanosoma cruzi, the protozoan causative of Chagas disease was published more than a decade ago. However, due to their complexity, its complete haploid predicted sequence and therefore its genetic repertoire remains unconfirmed. In this work, we have used RNAseq data to improve the previous genome assembly of Sylvio X10 strain and to define the complete transcriptome at trypomastigote stage (mammalian stage). A total of 22,977 transcripts were identified, of which more than half could be considered novel as they did not match previously annotated genes. Moreover, for the first time in T. cruzi, we are providing their relative abundance levels. We have identified that Sylvio X10 trypomastigotes exhibit a predominance of surface protein genes, specifically those encoding trans-sialidase and mucin-like proteins. On the other hand, detailed analysis of the pre-mRNA processing sites revealed some similarities but also some differences in the spliced leader and different polyadenylation addition sites compared to close related kinetoplastid parasites. Our results also confirm that transcription is bidirectional as occur in other kinetoplastids and the proportion of forward-sense and reverse-sense transcripts is almost equivalent, demonstrating that a strand-specificity does not exist.

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