In vitro assessment of Argemone mexicana, Taraxacum officinale, Ruta chalepensis and Tagetes filifolia against Haemonchus contortus nematode eggs and infective (L3) larvae

2017 ◽  
Vol 109 ◽  
pp. 162-168 ◽  
Author(s):  
Gabriela Jasso Díaz ◽  
Glafiro Torres Hernández ◽  
Alejandro Zamilpa ◽  
Carlos Miguel Becerril Pérez ◽  
J. Efrén Ramírez Bribiesca ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Taraxacum Officinale ◽  
Argemone Mexicana ◽  
Ruta Chalepensis ◽  
Nematode Eggs ◽  
In Vitro Assessment

scholarly journals Anthelmintic Activity of Wormwood (Artemisia absinthium L.) and Mallow (Malva sylvestris L.) against Haemonchus contortus in Sheep

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 219 ◽  
Author(s):  
Dominika Mravčáková ◽  
Michaela Komáromyová ◽  
Michal Babják ◽  
Michaela Urda Dolinská ◽  
Alžbeta Königová ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Small Ruminants ◽  
Anthelmintic Activity ◽  
In Vitro Tests ◽  
Artemisia Absinthium ◽  
Egg Hatch ◽  
Faecal Samples ◽  
Ovicidal Effect ◽  
Nematode Eggs

The objective of this study is to evaluate the effect of dry wormwood and mallow on the gastrointestinal parasite of small ruminants Haemonchus contortus. Twenty-four experimentally infected lambs were randomly divided into four groups of six animals each: unsupplemented lambs, lambs supplemented with wormwood, lambs supplemented with mallow and animals supplemented with a mix of both plants. Faecal samples from the lambs were collected on day 23, 29, 36, 43, 50, 57, 64 and 75 post-infection for quantification of the number of eggs per gram (EPG). The mix of both plants contained phenolic acids (10.7 g/kg DM) and flavonoids (5.51 g/kg DM). The nematode eggs were collected and in vitro egg hatch test was performed. The aqueous extracts of both plants exhibited strong ovicidal effect on H. contortus, with ED50 and ED99 values of 1.40 and 3.76 mg/mL and 2.17 and 5.89 mg/mL, respectively, in the in vitro tests. Despite the great individual differences between the treated lambs in eggs reduction, the mean EPG of the untreated and treated groups did not differ (p > 0.05). Our results indicate that using wormwood and mallow as dietary supplements do not have a sufficient effect on lambs infected with H. contortus.

In vitro assessment of the efficacy of protein exudates from seeds against Haemonchus contortus

2021 ◽  
Vol 292 ◽  
pp. 109399
Author(s):  
Irlla Correia Lima Licá ◽  
Alexandra Martins Santos Soares ◽  
André Teixeira Silva Ferreira ◽  
Jonas Perales ◽  
Ivo Alexandre Leme Cunha ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
In Vitro Assessment

scholarly journals Age of Haemonchus contortus third stage infective larvae is a factor influencing the in vitro assessment of anthelmintic properties of tannin containing plant extracts

2017 ◽  
Vol 243 ◽  
pp. 130-134 ◽  
Author(s):  
G.S. Castañeda-Ramírez ◽  
C. Mathieu ◽  
G. Vilarem ◽  
H. Hoste ◽  
P. Mendoza-de-Gives ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Haemonchus Contortus ◽  
Infective Larvae ◽  
Third Stage ◽  
In Vitro Assessment

In vitro assessment of Labisia pumila and its constituents for interactions with CYPs, P-GP and PXR

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
VK Manda ◽  
OR Dale ◽  
C Awortwe ◽  
Z Ali ◽  
IA Khan ◽  
...  
Keyword(s):  
In Vitro Assessment ◽  
Labisia Pumila

EFECTO DE LA ADICION DE DOS NIVELES DE RUDA (Ruta chalepensis L.) SOBRE LA PRODUCCION DE GAS METANO in vitro

2018 ◽  
Vol 11 (11) ◽  
Author(s):  
A.A. Rayas-Amor
Keyword(s):  
Ruta Chalepensis

Objetivo: evaluar el efecto de la adición de dos niveles de hojas de ruda (Ruta chalepensis L.) con diferentes niveles de materia seca incubada sobre la mitigación de metano (CH4) y la digestibilidad de la materia seca in vitro. Diseño/metodología/aproximación: Los tratamientos evaluados consistieron en muestras de pasto nativo dominado por pasto kikuyo (Penisetum clandestinum) más la adición de 3 y 6% de hoja de ruda respecto a un control. El CH4 se cuantificó a las 12, 24 y 48 h, post-incubación. Resultados: se registraron diferencias significativas (P<0.01) entre tratamientos en todas las horas de medición; siendo los tratamientos PN0.57 R0%, PN1.19 R3%, PN1.95 R3% y PN2.18 R3% los que presentaron menor producción acumulada de CH4 a las 12, 24 y 48 h, respectivamente. Limitaciones del estudio/implicaciones: los resultados de este trabajo fueron obtenidos a partir de un estudio in vitro; por lo tanto, no son aplicables directamente a las emisiones entéricas in vivo, no obstante, se tiene una aproximación de cuanta energía metabolizable se puede perder en forma de metano. Hallazgos/conclusiones: las hojas de ruda presentaron mayor efecto de mitigación de CH4. En este tenor, los tratamientos PN1.95 R3% y PN2.18 R3% redujeron en 75% y 95% la tasa de producción de CH4 a las 24 y 48 h, con respecto a los tratamientos sin hoja de ruda, y se observó un aumento en la digestibilidad in vitro de la materia seca cuando se compararon los tratamientos con ruda y los tratamientos sin hoja de ésta.

Effect of Jatropha curcas Latex on L3 Haemonchus contortus Larval Motility

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Noorzaid Muhamad ◽  
Syahirah Sazeli ◽  
Resni Mona ◽  
Jannathul Firdous
Keyword(s):  
Larvicidal Activity ◽  
Jatropha Curcas ◽  
Haemonchus Contortus ◽  
Anthelmintic Resistance ◽  
Small Ruminants ◽  
Gastrointestinal Nematodes ◽  
Dose Dependent ◽  
Plants Extract

The anthelmintic resistance has limited the control of gastrointestinal nematodes of small ruminants and thus has awakened interest in the study of plants extract as a source of anthelmintics. These experiments were carried out to evaluate the in vitro efficacy of Jatrophacurcas latex extract against Haemonchuscontortus larval motility. To evaluate the larvicidal activity, H.contortus L3 were incubated with the extracts with varying concentration of 5 mg/mL, 10 mg/mL, 15 mg/mL and 20 mg/mL at 27°C for 48, 72 and 96 hrs. The results were subjected to the Kruskal-Wallis test (P less than 0.05). The extracts showed dose-dependent larvicidal effects. These results suggest that J.curcas can be used to control gastrointestinal nematodes of small ruminants.

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