Evaluation of oxidative/nitrosative stress biomarkers and DNA damage in buffaloes naturally infected with Theileria annulata

Abstract This study investigated the in vitro anthelmintic activity of copper oxide (CuO) and zinc oxide (ZnO) nanoparticles (NPs) against Marshallagia marshalli. The in vitro study was based on an egg hatch assay, adult and larvae motility inhibition assays, DNA damage, intensity protein profile along with several oxidative/nitrosative stress biomarkers including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), protein carbonylation (PCO), malondialdehyde (MDA), total antioxidant status (TAS) and nitric oxide (NO) content. Different concentrations of CuO-NPs and ZnO-NPs (1, 4, 8, 12 and 16 ppm) were used to assess anthelmintic effects on three stages of M. marshalli life cycle – that is, eggs, larvae and adult parasites for 24 h. The results indicated that CuO-NPs and ZnO-NPs played a significant role as anthelminthics, and the effect was dependent on time and concentration. The concentrations of 12 and 16 ppm of CuO-NPs and 16 ppm of ZnO-NPs resulted in the induction of oxidative/nitrosative stress (decreased SOD, GSH-Px and CAT, and increased MDA, PCO and NO), increased DNA damage, inhibition of adult and larval motility, egg hatch and low intensity of protein bands following sodium dodecyl sulphate–polyacrylamide gel electrophoresis, compared to control. It was concluded that CuO-NPs and ZnO-NPs could be utilized as novel and potential agents for the control and treatment of M. marshalli infection, and they have the pharmacological potential to be studied in vivo for further utilization in treating parasitic infections.

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Early ROS-mediated DNA damage and oxidative stress biomarkers in Monoclonal B Lymphocytosis

Cancer Letters ◽

10.1016/j.canlet.2011.11.018 ◽

2012 ◽

Vol 317 (2) ◽

pp. 144-149 ◽

Cited By ~ 19

Author(s):

Rosa Collado ◽

Isabel Oliver ◽

Carmen Tormos ◽

Mercedes Egea ◽

Amparo Miguel ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

And Oxidative Stress

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The Investigation of Oxidative/nitrosative Stress and DNA Damage in Patients with Permanent Tunnel Port Catheters with Ultrasound Access to Bed

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2019.10.208 ◽

2019 ◽

Vol 145 ◽

pp. S78-S79

Keyword(s):

Dna Damage ◽

Nitrosative Stress ◽

Port Catheters

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Assessment of oxidative/nitrosative stress biomarkers and DNA damage in Haemonchus contortus, following exposure to zinc oxide nanoparticles

Acta Parasitologica ◽

10.1515/ap-2018-0065 ◽

2018 ◽

Vol 63 (3) ◽

pp. 563-571 ◽

Cited By ~ 6

Author(s):

Bijan Esmaeilnejad ◽

Awat Samiei ◽

Yousef Mirzaei ◽

Farhad Farhang-Pajuh

Keyword(s):

Dna Damage ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Metallic Nanoparticles ◽

Nitrosative Stress ◽

Total Antioxidant Status ◽

Parasitic Infections ◽

Oxide Nanoparticles ◽

Helminth Parasites ◽

Zno Nps

Abstract Drug resistance in helminth parasites has incurred several difficulties to livestock industry and ranked among the top public health concerns. Therefore, seeking for new agents to control parasites is an urgent strategy. In the recent years, metallic nanoparticles have been considerably evaluated for anthelmintic effects. The current research was conducted to assess possible anthelmintic impacts of zinc oxide nanoparticles (ZnO-NPs) on a prevalent gastrointestinal nematode, H. contortus. Moreover, several biomarkers of oxidative/nitrosative stress and DNA damage were measured. Various concentrations of the nanoparticle were prepared and incubated with the worms for 24 hours. The parasite mobility, mortality rate, antioxidant enzymes activities (SOD, Catalase and GSH-Px), lipid peroxidation, total antioxidant status as well as nitric oxide (NO) contents and DNA damage were determined. ZnO-NPs exerted significant wormicidal effects via induction of oxidative/nitrosative stress and DNA damage. Conclusively, ZnO-NPs can be utilized as a novel and potential agent to control and treatment of helminth parasitic infections.

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Oxidative and nitrosative stress in acute renal ischemia

AJP Renal Physiology ◽

10.1152/ajprenal.0071.2001 ◽

2001 ◽

Vol 281 (5) ◽

pp. F948-F957 ◽

Cited By ~ 203

Author(s):

Eisei Noiri ◽

Akihide Nakao ◽

Koji Uchida ◽

Hirokazu Tsukahara ◽

Minoru Ohno ◽

...

Keyword(s):

Nitric Oxide ◽

Lipid Peroxidation ◽

Dna Damage ◽

Oxidative Dna Damage ◽

Nitrosative Stress ◽

Oxidative And Nitrosative Stress ◽

Reperfusion Period ◽

Series Of Experiments ◽

Acute Renal Ischemia ◽

Oxide Synthase

First Published July 12, 2001; 10.1152/ajprenal.0071.2001.—Generation of reactive oxygen species and nitric oxide in hypoxia-reperfusion injury may form a cytotoxic metabolite, peroxynitrite, which is capable of causing lipid peroxidation and DNA damage. This study was designed to examine the contribution of oxidative and nitrosative stress to the renal damage in ischemic acute renal failure (iARF). iARF was initiated in rats by 45-min renal artery clamping. This resulted in lipid peroxidation, DNA damage, and nitrotyrosine modification confirmed both by Western and immunohistochemical analyses. Three groups of animals were randomly treated with an inhibitor of inducible nitric oxide synthase (NOS),l- N 6-(1-iminoethyl)lysine (l-Nil), cell-permeable lecithinized superoxide dismutase (SOD), or both. Each treatment resulted in amelioration of renal dysfunction, as well as reduced nitrotyrosine formation, lipid peroxidation, and DNA damage, thus suggesting that peroxynitrite rather than superoxide anion is responsible for lipid peroxidation and DNA damage. Therefore, in a separate series of experiments, a scavenger of peroxynitrite, ebselen, was administered before the reperfusion period. This treatment resulted in a comparable degree of amelioration of iARF. In conclusion, the present study provides the first attempt to elucidate the role of peroxynitrite in initiation of the cascade of lipid peroxidation and DNA damage to ischemic kidneys. The results demonstrate that l-Nil , lecithinized SOD, and ebselen treatments improve renal function due to their suppression of peroxynitrite production or its scavenging, consequently preventing lipid peroxidation and oxidative DNA damage.

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Variations in systemic biomarkers of oxidative/nitrosative stress and DNA damage before and during the consequent two cycles of chemotherapy in breast cancer patients

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2010.249 ◽

2010 ◽

Vol 48 (10) ◽

Cited By ~ 7

Author(s):

Pinar Atukeren ◽

Berna Yavuz ◽

Hilal Oguz Soydinc ◽

Sevim Purisa ◽

Hakan Camlica ◽

...

Keyword(s):

Breast Cancer ◽

Dna Damage ◽

Cancer Patients ◽

Nitrosative Stress ◽

Breast Cancer Patients

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Immune-Inflammatory, Metabolic, Oxidative, and Nitrosative Stress Biomarkers Predict Acute Ischemic Stroke and Short-Term Outcome

Neurotoxicity Research ◽

10.1007/s12640-020-00221-0 ◽

2020 ◽

Vol 38 (2) ◽

pp. 330-343 ◽

Cited By ~ 1

Author(s):

Daniela Frizon Alfieri ◽

Marcio Francisco Lehmann ◽

Tamires Flauzino ◽

Maria Caroline Martins de Araújo ◽

Nicolas Pivoto ◽

...

Keyword(s):

Ischemic Stroke ◽

Acute Ischemic Stroke ◽

Nitrosative Stress ◽

Short Term ◽

Oxidative And Nitrosative Stress ◽

Term Outcome ◽

Stress Biomarkers ◽

Short Term Outcome

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Assessment of oxidative/nitrosative stress biomarkers and DNA damage in Teladorsagia circumcincta following exposure to zinc oxide nanoparticles

Journal of Helminthology ◽

10.1017/s0022149x19001068 ◽

2020 ◽

Vol 94 ◽

Author(s):

Z. Baghbani ◽

B. Esmaeilnejad ◽

S. Asri-Rezaei

Keyword(s):

Dna Damage ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Nitrosative Stress ◽

Gastrointestinal Nematodes ◽

Parasitic Infections ◽

Oxide Nanoparticles ◽

Helminth Parasites ◽

Zno Nps ◽

Teladorsagia Circumcincta

Abstract Drug resistance to helminth parasites is one of the most serious problems to threaten the livestock industry. The problem also poses a major threat to public health. Therefore, novel and safe agents should urgently be investigated to control parasitic infections. The current study was conducted to evaluate the possible antiparasitic effects of zinc oxide nanoparticles (ZnO-NPs) on one of the most prevalent gastrointestinal nematodes, Teladorsagia circumcincta. The worms were incubated with various concentrations of ZnO-NPs: 1, 4, 8, 12 and 16 ppm for 24 hours. Mobility and mortality of the parasites were recorded at four-hour intervals. At the endpoint, several biomarkers of oxidative/nitrosative stress, including superoxide dismutase, glutathione peroxidase and catalase, as well as lipid peroxidation, protein carbonylation, total antioxidant status, nitric oxide contents and DNA damage, were measured in the homogenized samples. ZnO-NPs showed significant anthelminthic effects, depending on time and concentration. Furthermore, the nanoparticle induced severe oxidative/nitrosative stress and DNA damage. ZnO-NPs could be considered as a novel and potent anthelminthic agent.

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A randomized trial of short-term treatment with folic acid to reduce the oxidative stress of patients with chronic kidney disease

Current Drug Metabolism ◽

10.2174/1389200222666211210153145 ◽

2021 ◽

Vol 22 ◽

Author(s):

Andressa Keiko Matsumoto ◽

Ana Paula Michelin ◽

Laura de Oliveira Semeão ◽

Walter Sepúlveda-Loyolaa ◽

João Victor de Lima Pedrão ◽

...

Keyword(s):

Oxidative Stress ◽

Chronic Kidney Disease ◽

Folic Acid ◽

Kidney Disease ◽

Acid Treatment ◽

Nitrosative Stress ◽

Intervention Group ◽

Active Intervention ◽

Stress Biomarkers ◽

Active Intervention Group

Background: Increased generation of reactive oxygen and nitrogen species in chronic kidney disease (CKD) patients leads to increased oxidative stress. The antioxidant capacity of folic acid has been shown to scavenge radicals efficiently. Objective: The current study was carried out to examine the effects of folic acid treatment on biochemical and oxidative stress biomarkers in patients in different stages of CKD. Methods: This was a randomized, non-blinded, clinical trial that assessed the effects of 3 months of treatment with 5 mg of folic acid daily or no treatment in 113 outpatients within CKD stages 3a and 3b. At the end of the intervention, we analyzed the data of 66 patients treated with folic acid and 47 in the control group. Serum homocysteine levels and biochemical and oxidative/nitrosative stress biomarkers were analyzed in all patients. Results: In most patients, folic acid treatment normalized homocysteine levels and increased antioxidant enzyme activity (paraoxonase 1) and decreased sulfhydryl (SH) groups. In addition, oxidative biomarkers (products of nitric oxide and lipid hydroperoxide) were significantly lower post-treatment compared to baseline in the active intervention group. In the no active intervention group, no statistically significant effects were found on the oxidative and biochemical biomarkers. Conclusion: Folic acid treatment in stages 3a-4 CKD patients effectively ameliorated their hyperhomocysteinemia and increased the activity of antioxidant enzymes, as well as decreased the levels of pro-oxidant biomarkers in stage G3a and G3b CKD patients. Folic acid treatment attenuated oxidative/nitrosative stress and may be considered as a possible strategy to improve redox status and diminish the damages associated with oxidative/nitrosative stress in CKD patients. Further studies are needed to confirm these findings.

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