Comparative analysis of the mitochondrial proteins reveals complex structural and functional relationships in Fasciola species

2021 ◽  
Vol 152 ◽  
pp. 104754
Author(s):  
Hafiz Ishfaq Ahmad ◽  
Muhammad Bilal Bin Majeed ◽  
Muhammad Zulfiqar Ahmad ◽  
Abdul Jabbar ◽  
Babar Maqbool ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Mitochondrial Proteins ◽  
Functional Relationships ◽  
Fasciola Species ◽  
Complex Structural

scholarly journals Comparative Analysis of the Mitochondrial Proteins Reveals Complex Structural and Functional Relationships in Fasciola Species

2020 ◽  
Author(s):  
Hafiz Ishfaq Ahmad ◽  
Muhammad Bilal Bin Majeed ◽  
Muhammad Ijaz ◽  
Muhammad Zulfiqar Ahmad ◽  
Babar Maqbool ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mitochondrial Protein ◽  
Experimental Studies ◽  
Amino Acid Sequences ◽  
Mitochondrial Proteins ◽  
Dimensional Structure ◽  
Simultaneous Exposure ◽  
Diversity Pattern ◽  
Functional Relationships ◽  
Fasciola Species

Mitochondria is a cellular source of energy, playing an essential role in cellular stress induced by environmental stimuli. The genetic diversity of mitochondrial genes involved in oxidative phosphorylation affects the production of cellular energy and regional adaptation to various ecological (climatic) pressures influencing amino acid sequences (variants of protein). However, a little is known about the combined effect of protein changes on cell-level metabolic alterations in simultaneous exposure to various environmental conditions, including mitochondrial dysfunction and oxidative stress induction. Present study was designed to address this issue by analyzing the mitochondrial proteins in Fasciola species including Cytochrome C oxidase (COX1, COX2, COX3 and CYTB) and NADH dehydrogenase (ND1, ND2, ND3, ND4, ND5 and ND6). Mitochondrial proteins were used for a detailed computational investigation using available standard bioinformatics tools to explore structural and functional relationships. Our analysis shows that the mitochondrial protein family of Fasciola species are extensively diversified in all species studied, showing an extending role in various biological processes The results showed that the protein of COX1 of F. hepatica, F. gigantica and F. jacksoni consist of 510, 513 and 517 amino acids respectively. The alignment of proteins showed that these proteins are conserved in the same regions at ten positions in COX and CYTB proteins while at twelve locations in NADH. Three dimensional structure of COX, CYTB and NADH proteins were compared and the differences in additional conserved and binding sites in COX and CYTB proteins as compared to NADH were found in three Fasciola species. These results, based on the amino acid diversity pattern, were used to identify sites in the enzyme and the variations in mitochondrial proteins among Fasciola species. This study provides valuable information for future experimental studies including identification of therapeutics, diagnostics and immunoprophylactic interests with novel mitochondrial proteins.

scholarly journals Comparative analysis reveals no consistent association between the secondary structure of the 3′-untranslated region of dengue viruses and disease syndrome

2006 ◽  
Vol 87 (9) ◽  
pp. 2595-2603 ◽  
Author(s):  
Yang Zhou ◽  
Mammen P. Mammen ◽  
Chonticha Klungthong ◽  
Piyawan Chinnawirotpisan ◽  
David W. Vaughn ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Comparative Analysis ◽  
Secondary Structure ◽  
Untranslated Region ◽  
Denv Infection ◽  
Viral Fitness ◽  
Severe Dengue ◽  
Dengue Disease ◽  
Structural Interactions ◽  
Complex Structural

A comparative analysis was performed of the 3′-untranslated region (UTR) of Dengue virus (DENV) sampled from Bangkok, Thailand, over a 30 year period and representing all four serotypes. Considerable genetic variation was observed both within and among serotypes. Notably, a full-length version of the critical 3′-long stable hairpin structure was absent from some isolates, suggesting the occurrence of complex structural interactions within the 3′-UTR, including the influence of upstream mutations. The Thai sequences were then combined with 61 globally sampled isolates of DENV taken from patients with either dengue fever or severe dengue disease. No consistent association was found between 3′-UTR secondary structure and the clinical outcome of DENV infection, although some evidence for a trend in this direction was observed in DENV-2. It was concluded that the 3′-UTR is not the sole determinant of DENV virulence in nature, although variation in secondary structure may greatly influence viral fitness.

scholarly journals A Comparative Analysis of the Proteins and Lipids in Breast, Leg, and Thigh Meats of a Commercial Domesticated Turkey

2017 ◽  
Author(s):  
W. Keith Ray ◽  
Sherry B. Hildreth ◽  
Judith M. Jervis ◽  
Richard F. Helm
Keyword(s):  
Comparative Analysis ◽  
Breast Tissue ◽  
Meleagris Gallopavo ◽  
Mitochondrial Proteins ◽  
Oxidized Lipids ◽  
Local Market ◽  
Surface Areas ◽  
Potential Marker ◽  
Muscle Formation ◽  
Glycerol 3 Phosphate Dehydrogenase

A commercially reared domesticated turkey (Meleagris gallopavo) was purchased from a local market and sections of tissue representing leg, thigh, and breast were harvested and processed for analysis of the lipids and proteins present. Leg and thigh tissue was enriched in mitochondrial proteins whereas the breast tissue was enriched in glycolytic enzymes as well as the cytosolic and mitochondrial forms of glycerol-3-phosphate dehydrogenase. A potential marker for breast tissue muscle formation and/or function was also identified. The tissues could clearly be separated based upon their lipid profiles with little differences in cardiolipin levels suggesting that mitochondrial surface areas may be similar across the tissues. The most significant differences in the lipids were found to be higher levels of oxidized lipids in thigh meat. This work provides the first untargeted proteome and lipidome datasets for the domesticated turkey. The proteome dataset is accessible from ProteomeXchange Consortium via the PRIDE partner repository with the identifier PXD008207.

Three-dimensional organization and functional relationships of endocytotic compartments in pig intestinal epithelial cells

1992 ◽  
Vol 50 (1) ◽  
pp. 576-577
Author(s):  
Julian P. Heath ◽  
Buford L. Nichols ◽  
László G. Kömüves
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Three Dimensional ◽  
Intestinal Epithelial ◽  
Cell Surfaces ◽  
Basal Surface ◽  
Functional Relationships

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.

1207: Failed Pyeloplasty in Children: Comparative Analysis of Retrograde Endopyelotomy VS. Re-do Pyeloplasty

2007 ◽  
Vol 177 (4S) ◽  
pp. 398-398
Author(s):  
Luis H. Braga ◽  
Joao L. Pippi Salle ◽  
Sumit Dave ◽  
Sean Skeldon ◽  
Armando J. Lorenzo ◽  
...  
Keyword(s):  
Comparative Analysis
Sign in / Sign up

Export Citation Format

Share Document