A new antimicrobial susceptibility testing method of Escherichia coli against ampicillin by MSPQC

This study estimated the prevalence of Salmonella, Campylobacter, and Escherichia coli isolates in fresh retail grain-fed veal obtained in Ontario, Canada. The prevalence and antimicrobial resistance patterns were examined for points of public health significance. Veal samples (n = 528) were collected from February 2003 through May 2004. Twenty-one Salmonella isolates were recovered from 18 (4%) of 438 samples and underwent antimicrobial susceptibility testing. Resistance to one or more antimicrobials was found in 6 (29%) of 21 Salmonella isolates; 5 (24%) of 21 isolates were resistant to five or more antimicrobials. No resistance to antimicrobials of very high human health importance was observed. Ampicillin-chloramphenicol-streptomycin-sulfamethoxazole-tetracycline resistance was found in 5 (3%) of 21 Salmonella isolates. Campylobacter isolates were recovered from 5 (1%) of 438 samples; 6 isolates underwent antimicrobial susceptibility testing. Resistance to one or more antimicrobials was documented in 3 (50%) of 6 Campylobacter isolates. No Campylobacter isolates were resistant to five or more antimicrobials or category I antimicrobials. E. coli isolates were recovered from 387 (88%) of 438 samples; 1,258 isolates underwent antimicrobial susceptibility testing. Resistance to one or more antimicrobials was found in 678 (54%) of 1,258 E. coli isolates; 128 (10%) of 1,258 were resistant to five or more antimicrobials. Five (0.4%) and 7 (0.6%) of 1,258 E. coli isolates were resistant to ceftiofur and ceftriaxone, respectively, while 34 (3%) of 1,258 were resistant to nalidixic acid. Ciprofloxacin resistance was not detected. There were 101 different resistance patterns observed among E. coli isolates; resistance to tetracycline alone (12.7%, 161 of 1,258) was most frequently observed. This study provides baseline prevalence and antimicrobial resistance data and highlights potential public health concerns.

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Bacterial Cytological Profiling (BCP) as a Rapid and Accurate Antimicrobial Susceptibility Testing Method for Staphylococcus aureus

EBioMedicine ◽

10.1016/j.ebiom.2016.01.020 ◽

2016 ◽

Vol 4 ◽

pp. 95-103 ◽

Cited By ~ 25

Author(s):

D.T. Quach ◽

G. Sakoulas ◽

V. Nizet ◽

J. Pogliano ◽

K. Pogliano

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Susceptibility ◽

Susceptibility Testing ◽

Antimicrobial Susceptibility Testing ◽

Testing Method ◽

Bacterial Cytological Profiling ◽

Cytological Profiling

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Antibiotic Resistance among Escherichia coli Isolates from Hospital Wastewater Compared to Community Wastewater

Water ◽

10.3390/w13233449 ◽

2021 ◽

Vol 13 (23) ◽

pp. 3449

Author(s):

Cristina-Mirabela Gaşpar ◽

Ludovic Toma Cziszter ◽

Cristian Florin Lăzărescu ◽

Ioan Ţibru ◽

Marius Pentea ◽

...

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Antimicrobial Susceptibility ◽

Susceptibility Testing ◽

Antimicrobial Susceptibility Testing ◽

Diffusion Method ◽

Resistant Bacteria ◽

Hospital Wastewater ◽

Extended Spectrum Beta Lactamase ◽

E Coli

This study aimed to compare the antibiotic resistance levels of the indicator bacteria Escherichia coli in wastewater samples collected from two hospitals and two urban communities. Antimicrobial susceptibility testing was performed on 81 E. coli isolates (47 from hospitals and 34 from communities) using the disc diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) methodology. Ten antibiotics from nine different classes were chosen. The strains isolated from the community wastewater, compared to those from the hospital wastewater, were not resistant to gentamicin (p = 0.03), but they showed a significantly higher susceptibility—increased exposure to ceftazidime (p = 0.001). Multidrug resistance was observed in 85.11% of the hospital wastewater isolates and 73.53% of the community isolates (p > 0.05). The frequency of the presumed carbapenemase-producing E. coli was higher among the community isolates (76.47% compared to 68.09%) (p > 0.05), whereas the frequency of the presumed extended-spectrum beta-lactamase (ESBL)-producing E. coli was higher among the hospital isolates (21.28% compared to 5.88%) (p > 0.05). The antibiotic resistance rates were high in both the hospital and community wastewaters, with very few significant differences between them, so the community outlet might be a source of resistant bacteria that is at least as important as the well-recognised hospitals.

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ENTERIC BACTERIA IN FECAL SAMPLES OF EURASIAN GRIFFON VULTURES

Archives of Veterinary Medicine ◽

10.46784/e-avm.v6i2.153 ◽

2014 ◽

Vol 6 (2) ◽

pp. 45-53

Author(s):

Dubravka Milanov ◽

Dragan Fabijan ◽

Bojana Prunić ◽

Maja Velhner ◽

Tamaš Petrović

Keyword(s):

Escherichia Coli ◽

Antimicrobial Susceptibility ◽

Salmonella Enterica ◽

Susceptibility Testing ◽

Enteric Bacteria ◽

Antimicrobial Susceptibility Testing ◽

Salmonella Spp ◽

Fecal Samples ◽

E Coli

Fecal samples originating from 15 Eurasian griffon vultures were collected during June 2012 in the territory of special nature reservation Uvac and examined for presence of enteric bacteria Escherichia coli and Salmonella spp. Salmonellas were isolated from five samples (33.3%) and serologically typed as Salmonella enterica subsp. enterica ser. Veneziana. E. coli was isolated from four samples (26.6%). Antimicrobial susceptibility testing revealed resistance to one and more antibiotics only in E. coli isolates.

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