A rapid detection method using flow cytometry to monitor the risk of Legionella in bath water

The migration rules of two triazine type of UV absorbents (UV-1577 and UV-1579) from polypropylene (PP) food contact materials to food simulant were investigated. A rapid detection method of UV-1577 and UV-1579 in PP food contact materials was established by ultra-performance convergence chromatography (UPC2). The optimal extraction method of the initial solution was determined, and the pretreatment methods of two UV absorbents in the liquid simulants were optimized. Experiment results showed that the interaction effects between the two UV absorbers and the liquid simulants were weak, and the migration behavior was the most obvious in the fatty simulant of isooctane. At the same temperature, the migration amount of both UV-1577 and UV-1579 increased with the extension of time, and UV-1577 was more prone to migration than UV-1579. Within a certain range, the migration amount of the two UV absorbents increased significantly with the increase of the migration temperature. The higher the temperature was, the shorter the time required to reach the equilibrium.

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Application of single-cell real-time imaging flow cytometry in rapid detection of pathogenic fungi in clinical liquid specimens

Methods and Applications in Fluorescence ◽

10.1088/2050-6120/abe7ca ◽

2021 ◽

Vol 9 (2) ◽

pp. 025004

Author(s):

Linting Lv ◽

Li Dong ◽

Jiajia Zheng ◽

Tuohutaerbieke Maermaer ◽

Xiangbo Huang ◽

...

Keyword(s):

Flow Cytometry ◽

Single Cell ◽

Real Time ◽

Rapid Detection ◽

Pathogenic Fungi ◽

Real Time Imaging ◽

Imaging Flow Cytometry

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Rapid Detection of Chloramphenicol Residues in Fermented Foods Based on UPLC-DAD

Tobacco Regulatory Science ◽

10.18001/trs.7.6.105 ◽

2021 ◽

Vol 7 (6) ◽

pp. 6303-6316

Author(s):

Weixi Gao ◽

Yan Zhuang

Keyword(s):

Rapid Detection ◽

Detection Method ◽

Original Method ◽

Fermented Food ◽

Fermented Foods ◽

Test Paper ◽

Set Up ◽

Test Process ◽

Detection Reagent ◽

Detection Speed

in the detection of chloramphenicol residues in fermented food, there are often problems of slow detection speed. Using UPLC-DAD method, a rapid detection method of chloramphenicol residues in fermented food based on UPLC-DAD method is designed. According to the characteristics of chloramphenicol, set up the detection reagent, select the detection equipment, and form the detection laboratory. It is usingUPLC-DAD method to design the test paper, using the set test reagent to deal with the sample to be tested, according to the design results of the test process, combining the reagent with the sample, to determine its specificity. Chloramphenicol residue was detected by test paper. So far, the rapid detection method of chloramphenicol residues in fermented food based on UPLC-DAD method has been designed. Compared with the original detection method, the detection speed of the detection method designed in this paper is significantly higher than the original method. In conclusion, the rapid detection method of chloramphenicol residues in fermented food based on UPLC-DAD method is effective.

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