Rapid detection of fosfomycin resistance in Escherichia coli and Klebsiella spp. strains isolated from urinary tract infections

2021 ◽  
pp. 106296
Author(s):  
N. Kansak ◽  
N. Arıcı ◽  
R. Adaleti ◽  
Y. Nakipoglu ◽  
S. Aksaray
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Rapid Detection ◽  
Fosfomycin Resistance ◽  
Tract Infections ◽  
Klebsiella Spp

scholarly journals Changing pattern of resistant pathogens causing urinary tract infections in Karachi

2013 ◽  
Vol 2 (3) ◽  
pp. 105-110
Author(s):  
H Najmul ◽  
A Tanveer
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Drugs ◽  
Klebsiella Species ◽  
Amoxicillin Clavulanic Acid ◽  
Tract Infections ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

INTRODUCTION: The study under view is based under the aim to investigate the prevalence and susceptibility pattern of pathogens, causing urinary tract infections (UTIs), to antibiotics commonly used in routine medication. MATERIALS AND METHODS: Over a period of 10 months 100 isolates were collected for the determination of their susceptibility to chosen antibiotics, from a laboratory (MedPath Laboratories) in urban area of Karachi. All Gramnegative and Gram-positive urinary tract pathogens were re-identified by their morphological and biochemical characteristics and the susceptibility to seven antibiotics was determined. RESULTS: Pathogens were found as, Escherichia coli, Pseudomona spp, Klebsiella species, Enterobacter spp., and Staphylococci spp. In recent study, more than half of the Escherichia coli isolates were resistant to one or more of the all antimicrobial drugs tested. Resistance was most common to amoxicillin/clavulanic acid and ofloxacin, cefixime, followed by gentamicin. Our results indicate that Escherichia coli and Pseudomonas spp. were the most common organisms causing UTI. Other organisms involved were Enterobacter spp., Staphylococcus spp., and Klebsiella spp. Increasing patterns of resistant to gentamicin, and ofloxacin were also observed. CONCLUSIONS: In conclusion, pattern of antibiotic susceptibility to first line antibiotics is changing hence antimicrobial susceptibility testing of all isolates is crucial for the treatment of UTI. DOI: http://dx.doi.org/10.3126/ijim.v2i3.8069 Int J Infect Microbiol 2013;2(3):99-104  

scholarly journals Gut microbiota and urinary tract infections in children

2021 ◽  
Vol 6 (2) ◽  
pp. 24-30
Author(s):  
L. A. Levanova ◽  
A. A. Markovskaya ◽  
L. Yu. Otdushkina ◽  
Yu. V. Zakharova
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Urinary Tract Infections ◽  
Intestinal Microbiota ◽  
Neonatal Intensive Care ◽  
Intestinal Microbiome ◽  
Intestinal Dysbiosis ◽  
Tract Infections ◽  
Klebsiella Spp

Aim. To evaluate the role of intestinal dysbiosis in the development of urinary tract infections in children admitted to a multidisciplinary hospital.Materials and Methods. We performed a bacteriological analysis of 2,694 urine samples collected from ≤ 3-years-old children who have been admitted to a multidisciplinary hospital (Kemerovo, Russian Federation). Urine specimens were inoculated by the 4-sector technique. Concurrently, we quantified the intestinal microbiota and its antimicrobial resistance in 100 children with urinary tract infections.Results. Titers of pathogenic microbes significantly differed in patients from distinct units (p = 0.02). The highest number of positive samples was detected in the neonatal pathology and neonatal intensive care units (43.8% each) as well as urology unit (37.9%). The most frequent pathogens belonged to Enterobacteriaceae family, in particular Escherichia coli, Klebsiella spp., Enterobacter spp., and Proteus spp. All studied children (100/100) suffered from intestinal dysbiosis. Titers of Bifidobacterium spp. and Lactobacillus spp. were reduced to 6 (5.0; 8.0) and 5 (4.0; 6.0) lg CFU/g, respectively. In 89.2% children, Escherichia coli lac+ levels were elevated to 9-10 lg CFU/g. Of note, 18.9% children had high Escherichia coli lac- titers [8 (6.0; 9.0) lg] and 24.3% had high Escherichia coli hly+ titers [5 (4.5; 6.0) lg] in the intestine. We have also found a high frequency (44.6%) and density [8.1 (7.0; 8.5) lg] of Klebsiella spp., in the intestinal mucosa of such patients. Notably, Enterobacter spp. and Proteus spp. were abundant [7 (5.0; 8.0) CFU/g] in the intestinal microbiota of 10.8% children. The prevalence of resistance strains in the studied setting reached 63%.Conclusions. More than 75% children with urinary tract infections suffer from intestinal dysbiosis. The microbiome of these patients was predominantly composed of Enterobacteriaceae and was characterized by high titers of Escherichia coli lac+, Klebsiella spp., Enterobacte spp., and Proteus spp. Similar profile of antimicrobial resistance in urinary and intestinal isolates of enterobacteria suggests intestinal microbiome as the main source of pathogens causing urinary tract infections in children. 

Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections

2019 ◽  
Vol 19 (3) ◽  
pp. 322-326 ◽  
Author(s):  
Hassan Valadbeigi ◽  
Elham Esmaeeli ◽  
Sobhan Ghafourian ◽  
Abbas Maleki ◽  
Nourkhoda Sadeghifard
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multiplex Polymerase Chain Reaction ◽  
Virulence Genes ◽  
Uropathogenic Escherichia Coli ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Tract Infections

Introduction: The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam. Materials and Methods: For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes. Results: The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained. Conclusion: Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.

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