Lipidomic Analysis Reveals the Importance of GIPCs in Arabidopsis Leaf Extracellular Vesicles

Extracellular vesicles (EVs) are lipid bilayer nanovesicles secreted from almost all cells including cancer. Cancer-derived EVs contribute to cancer progression and malignancy via educating the surrounding normal cells. In breast cancer, epidemiological and experimental observations indicated that lipids are associated with cancer malignancy. However, lipid compositions of breast cancer EVs and their contributions to cancer progression are unexplored. In this study, we performed a widely targeted quantitative lipidomic analysis in cells and EVs derived from high- and low-metastatic triple-negative breast cancer cell lines, using supercritical fluid chromatography fast-scanning triple-quadrupole mass spectrometry. We demonstrated the differential lipid compositions between EVs and cells of their origin, and between high- and low-metastatic cell lines. Further, we demonstrated EVs from highly metastatic breast cancer accumulated unsaturated diacylglycerols (DGs) compared with EVs from lower-metastatic cells, without increasing the amount in cells. The EVs enriched with DGs could activate the protein kinase D signaling pathway in endothelial cells, which can lead to stimulated angiogenesis. Our results indicate that lipids are selectively loaded into breast cancer EVs to support tumor progression.

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Lipidomic analysis of adipose-derived extracellular vesicles reveals their potential as lipid mediators of obesity-associated metabolic complications

10.1101/2021.12.10.472057 ◽

2021 ◽

Author(s):

Alexia Blandin ◽

Gregory Hilairet ◽

Maharajah Ponnaiah ◽

Simon Ducheix ◽

Isabelle Dugail ◽

...

Keyword(s):

Extracellular Vesicles ◽

Principal Component ◽

Obese Mice ◽

Metabolic Complications ◽

Lipidomic Analysis ◽

Novel Biomarkers ◽

Metabolic Dysfunctions ◽

Visceral Adipose ◽

Specific Lipid ◽

Lean Conditions

Adipose extracellular vesicles (AdEV) transport lipids that could participate to the development of obesity-related metabolic dysfunctions. This study aimed to define mice AdEV lipid signature in either healthy or obesity context by a targeted LC-MS/MS approach. Distinct clustering of AdEV and visceral adipose tissue (VAT) lipidomes by principal component analysis reveals specific lipid composition of AdEV compared to source VAT. Comprehensive analysis identifies enrichment of ceramides and phosphatidylglycerols in AdEV compared to VAT in lean conditions. Lipid subspecies commonly enriched in AdEV highlight specific AdEV-lipid sorting. Obesity impacts AdEV lipidome, driving triacylglycerols and sphingomyelins enrichment in obese versus lean conditions. Obese mice AdEV also display elevated phosphatidylglycerols and acid arachidonic subspecies contents highlighting novel biomarkers and/or mediators of metabolic dysfunctions. Our study identifies specific lipid-fingerprints for plasma, VAT and AdEV that are informative of the metabolic status and underline the signaling capacity of lipids transported by AdEV in obesity-associated complications.

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Correction: Lipidomic Analysis of Extracellular Vesicles from the Pathogenic Phase of Paracoccidioides brasiliensis

PLoS ONE ◽

10.1371/annotation/08ed7ef4-7f80-4aed-9929-98d39c3ca83f ◽

2012 ◽

Vol 7 (10) ◽

Cited By ~ 6

Author(s):

Milene C. Vallejo ◽

Ernesto S. Nakayasu ◽

Larissa V. G. Longo ◽

Luciane Ganiko ◽

Felipe G. Lopes ◽

...

Keyword(s):

Extracellular Vesicles ◽

Paracoccidioides Brasiliensis ◽

Lipidomic Analysis

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Comparative Lipidomic Analysis of Extracellular Vesicles Derived from Lactobacillus plantarum APsulloc 331261 Living in Green Tea Leaves Using Liquid Chromatography-Mass Spectrometry

International Journal of Molecular Sciences ◽

10.3390/ijms21218076 ◽

2020 ◽

Vol 21 (21) ◽

pp. 8076

Author(s):

Hyoseon Kim ◽

Minjung Kim ◽

Kilsun Myoung ◽

Wanil Kim ◽

Jaeyoung Ko ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Lactobacillus Plantarum ◽

Green Tea ◽

Extracellular Vesicles ◽

Liquid Chromatography Mass Spectrometry ◽

Tea Leaves ◽

Lipidomic Analysis ◽

Chromatography Mass Spectrometry ◽

Green Tea Leaves

Lactobacillus plantarum is a popular probiotic species due to its safe and beneficial effects on humans; therefore, novel L. plantarum strains have been isolated and identified from various dietary products. Given that bacteria-derived extracellular vesicles (EVs) have been considered as efficient carriers of bioactive materials and shown to evoke cellular responses effectively, L. plantarum-derived EVs are expected to efficiently elicit health benefits. Herein, we identified L. plantarum APsulloc 331261 living in green tea leaves and isolated EVs from the culture medium. We performed quantitative lipidomic analysis of L. plantarum APsulloc 331261 derived EVs (LEVs) using liquid chromatography-mass spectrometry. In comparison to L. plantarum APsulloc 331261, in LEVs, 67 of 320 identified lipid species were significantly increased and 19 species were decreased. In particular, lysophosphatidylserine(18:4) and phosphatidylcholine(32:2) were critically increased, showing over 21-fold enrichment in LEVs. In addition, there was a notable difference between LEVs and the parent cells in the composition of phospholipids. Our results suggest that the lipidomic profile of bacteria-derived EVs is different from that of the parent cells in phospholipid content and composition. Given that lipids are important components of EVs, quantitative and comparative analyses of EV lipids may improve our understanding of vesicle biogenesis and lipid-mediated intercellular communication within or between living organisms.

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