Evaluation of cell-surface interaction using a 3D spheroid cell culture model on artificial extracellular matrices

2017 ◽  
Vol 73 ◽  
pp. 310-318 ◽  
Author(s):  
Wolfgang Metzger ◽  
Sandra Rother ◽  
Tim Pohlemann ◽  
Stephanie Möller ◽  
Matthias Schnabelrauch ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Surface ◽  
Surface Interaction ◽  
Extracellular Matrices ◽  
Cell Culture Model ◽  
Culture Model ◽  
Spheroid Cell Culture

Cell-free DNA in a three-dimensional spheroid cell culture model: A preliminary study

2017 ◽  
Vol 89 ◽  
pp. 182-192 ◽  
Author(s):  
Janine Aucamp ◽  
Carlemi Calitz ◽  
Abel J. Bronkhorst ◽  
Krzysztof Wrzesinski ◽  
Sias Hamman ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Cell Free Dna ◽  
Free Dna ◽  
Culture Model ◽  
Preliminary Study ◽  
Spheroid Cell Culture

1120: Citrate and Vitamin E Blunt the SWL-Induced Free Radical Surge in an In-Vitro MDCK Cell Culture Model

2004 ◽  
Vol 171 (4S) ◽  
pp. 295-295
Author(s):  
Fernando C. Delvecchio ◽  
Ricardo M. Brizuela ◽  
Karen J. Byer ◽  
W. Patrick Springhart ◽  
Saeed R. Khan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Free Radical ◽  
Vitamin E ◽  
Mdck Cell ◽  
Cell Culture Model ◽  
Culture Model

Determination of anti-oxidative effects of phytogenic substances using a porcine epithelial cell culture model

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
T Kaschubek ◽  
E Mayer ◽  
G Schatzmayr ◽  
K Teichmann
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Cell Culture Model ◽  
Epithelial Cell Culture ◽  
Culture Model ◽  
Oxidative Effects

Juglone Exerts Cytotoxic, Anti-proliferative and Anti-invasive Effects on Glioblastoma Multiforme in a Cell Culture Model

2016 ◽  
Vol 16 (9) ◽  
pp. 1190-1197 ◽  
Author(s):  
Dziugas Meskelevicius ◽  
Kastytis Sidlauskas ◽  
Ruta Bagdonaviciute ◽  
Julius Liobikas ◽  
Daiva Majiene
Keyword(s):  
Cell Culture ◽  
Glioblastoma Multiforme ◽  
Cell Culture Model ◽  
Culture Model ◽  
A Cell

scholarly journals An In Vitro Cell Culture Model for Pyoverdine-Mediated Virulence

Pathogens ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 9
Author(s):  
Donghoon Kang ◽  
Natalia V. Kirienko
Keyword(s):  
Cell Culture ◽  
Virulence Factors ◽  
Model Organisms ◽  
Cell Culture Model ◽  
Chronic Infections ◽  
In Vitro Cell Culture ◽  
Mitochondrial Homeostasis ◽  
Culture Model ◽  
Wide Range

Pseudomonas aeruginosa is a multidrug-resistant, opportunistic pathogen that utilizes a wide-range of virulence factors to cause acute, life-threatening infections in immunocompromised patients, especially those in intensive care units. It also causes debilitating chronic infections that shorten lives and worsen the quality of life for cystic fibrosis patients. One of the key virulence factors in P. aeruginosa is the siderophore pyoverdine, which provides the pathogen with iron during infection, regulates the production of secreted toxins, and disrupts host iron and mitochondrial homeostasis. These roles have been characterized in model organisms such as Caenorhabditis elegans and mice. However, an intermediary system, using cell culture to investigate the activity of this siderophore has been absent. In this report, we describe such a system, using murine macrophages treated with pyoverdine. We demonstrate that pyoverdine-rich filtrates from P. aeruginosa exhibit substantial cytotoxicity, and that the inhibition of pyoverdine production (genetic or chemical) is sufficient to mitigate virulence. Furthermore, consistent with previous observations made in C. elegans, pyoverdine translocates into cells and disrupts host mitochondrial homeostasis. Most importantly, we observe a strong correlation between pyoverdine production and virulence in P. aeruginosa clinical isolates, confirming pyoverdine’s value as a promising target for therapeutic intervention. This in vitro cell culture model will allow rapid validation of pyoverdine antivirulents in a simple but physiologically relevant manner.

Sign in / Sign up

Export Citation Format

Share Document