Modulatory effects of Cornus sanguinea L. mediated green synthesized silver nanoparticles on oxidative stress, COX-2/NOS2 and NFkB/pNFkB expressions in experimental inflammation in Wistar rats

Silver nanoparticles (SNPs) are widely invested in nanomedicine and consuming products due to their unique antimicrobial properties. However, little is known about the toxicity of these particles on human health. The present investigation was carried out to investigate the histological alterations induced in the lung tissues by 20±5 nm SNPs. Male albino Wistar rats were exposed to SNPs at a daily dose of 2 mg/kg for 21 days. Lung biopsies from all rats under study were subjected to histopathological examinations. Exposure to 20±5 nm SNPs induced the following pulmonary alterations: thickened alveolar wall, macrophages invasion and inflammatory cells infiltration, lymphatic follicles enlargement, pulmonary edema, alveolar hypersensitivity and interstitial congestion. Occasional atelectasis and fibrocytes proliferation were also detected. The findings of the present work might indicate that SNPs potentially trigger oxidative stress and alterations in the pulmonary tissues that may affect the function of the lungs.

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The Preventive Effects and Mode of Actions of Ulva Fasciata Synthesized Silver Nanoparticles in Doxorubicin-Induced Hepatotoxicity in Wistar Rats

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i24a31429 ◽

2021 ◽

pp. 24-48

Author(s):

Asmaa M. Mahmoud ◽

Osama M. Ahmed ◽

Ibraheem B. Mohamed ◽

Hanan A. Soliman ◽

Basant M. Mohamed

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Wistar Rats ◽

Inflammatory Cytokine ◽

Total Bilirubin Level ◽

Ethanolic Extract ◽

Albumin Level ◽

Concurrent Treatment ◽

Ulva Fasciata ◽

Male Wistar Rats

Background: Hepatotoxicity was one of the major side effects associated with doxorubicin treatment in cancer chemotherapy. The synthesized silver nanoparticles (AgNPs) from natural products such as algae especially green algae is one of the favorable means to minimize the deleterious effects of the chemotherapy. Thus, this study aimed to evaluate the preventive role of AgNPs synthesized by Ulva fasciata (U. fasciata) against doxorubicin-induced hepatotoxicity and oxidative stress in the liver of male Wistar rats. Materials and Methods: In the present study, the green macroalga U. fasciata ethanolic extract was used as reducing agents to reduce Ag ions to Ag0. Doxorubicin-injected male Wistar rats were concomitantly treated with U. fasciata ethanolic extract and AgNPs synthesized by U. fasciata extract (AgNPs/U. fasciata) 3 times/week by oral gavage for 6 weeks. Results: The results showed that male Wistar rats injected with doxorubicin showed a significant increase in ALT, ALP and GGT activities and total bilirubin level as well as a reduction in the serum albumin level. The concurrent treatments of doxorubicin-injected rats with U. fasciata ethanolic extract and AgNPs/U. fasciata significantly abrogate these alterations. The altered levels of tumor biomarkers CA19.9 and AFP as well as pro-inflammatory cytokine, TNF-α, and anti-inflammatory cytokine, IL-4, in doxorubicin-injected animals were significantly ameliorated by concurrent treatment with U. fasciata and AgNPs/U. fasciata. Moreover, the elevated mRNA expression of p53 significantly decreased by treatment. In association, the doxorubicin-induced deleterious histological changes represented by severe hydropic degenerative changes, steatosis, inflammatory cell infiltration, Kupffer cell proliferation and apoptosis were remarkably improved by concurrent treatment with U. fasciata extract and AgNPs/U. fasciata which was more potent. Conclusion: Based on results of this study, it can be concluded that U. fasciata extract and AgNPs/U. fasciata counteracts doxorubicin-induced toxicity by suppression of inflammation, oxidative stress and apoptosis. AgNPs/U. fasciata was the most potent in improving hepatocyte integrity and liver histological architecture. Graphical Abstract

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Phytol Reduces Oxidative Stress and Cyclooxygenase-2 Expression in Kidney of Diabetic Wistar Rats

Recent Advances in Biology and Medicine ◽

10.18639/rabm.2018.04.643518 ◽

2018 ◽

Vol 04 ◽

pp. 16

Author(s):

Adeyomoye Olorunsola Israel ◽

Adewoye Elsie Olufunke ◽

◽

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Normal Control ◽

Wistar Rats ◽

Statistical Significance ◽

Kidney Tissue ◽

Diabetic Rats ◽

Cyclooxygenase 2 ◽

Sod Activity ◽

Cox 2

Diabetes mellitus is characterized by hyperglycemia, which induces oxidative stress and inflammation. The role of Phytol in oxidative stress and inflammation was investigated in diabetic rats. Fifteen Wistar rats were divided into five groups (n = 5). Groups 1, 2, and 3 served as normal control, diabetic untreated, and diabetic treated with 250 mg/kg Phytol, respectively. Rats were treated for 28 days with Phytol, and then blood samples were collected under sodium thiopental (30 mg/kg i.p) anesthesia for assay. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined using commercially available Randox kits. Cyclooxygenase-2 (COX-2) expressions in kidney samples were determined using immunostaining procedure. Statistical analysis was done using one-way analysis of variance and level of statistical significance taken at p < 0.05. Results showed a significant increase (p < 0.05) in CAT and GPx activities in diabetic treated with 250 mg/kg Phytol when compared with diabetic untreated with Phytol. SOD activity significantly decreased in diabetic untreated and diabetic treated with 250 mg/kg Phytol when compared with normal control. COX-2 was significantly expressed in diabetic untreated when compared with normal control and diabetic treated with 250 mg/kg Phytol. Oral administration of Phytol reduces oxidative stress damage and inflammation of kidney tissue caused by hyperglycemia in diabetes mellitus.

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Sex affects the response of Wistar rats to polyvinyl pyrrolidone (PVP)-coated silver nanoparticles in an oral 28 days repeated dose toxicity study

Particle and Fibre Toxicology ◽

10.1186/s12989-021-00425-y ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Marija Ćurlin ◽

Rinea Barbir ◽

Sanja Dabelić ◽

Marija Ljubojević ◽

Walter Goessler ◽

...

Keyword(s):

Oxidative Stress ◽

Silver Nanoparticles ◽

Stress Responses ◽

Wistar Rats ◽

Scientific Information ◽

Female Rats ◽

Repeated Dose ◽

Low Doses

Abstract Background Silver nanoparticles (AgNPs) are widely used in biomedicine due to their strong antimicrobial, antifungal, and antiviral activities. Concerns about their possible negative impacts on human and environmental health directed many researchers towards the assessment of the safety and toxicity of AgNPs in both in vitro and in vivo settings. A growing body of scientific information confirms that the biodistribution of AgNPs and their toxic effects vary depending on the particle size, coating, and dose as well as on the route of administration and duration of exposure. This study aimed to clarify the sex-related differences in the outcomes of oral 28 days repeated dose exposure to AgNPs. Methods Wistar rats of both sexes were gavaged daily using low doses (0.1 and 1 mg Ag/kg b.w.) of polyvinylpyrrolidone (PVP)-coated small-sized (10 nm) AgNPs. After exposure, blood and organs of all rats were analysed through biodistribution and accumulation of Ag, whereas the state of the liver and kidneys was evaluated by the levels of reactive oxygen species (ROS) and glutathione (GSH), catalase (CAT) activity, superoxide dismutase (SOD) and glutathione peroxidase (GPx), expression of metallothionein (Mt) genes and levels of Mt proteins. Results In all animals, changes in oxidative stress markers and blood parameters were observed indicating the toxicity of AgNPs applied orally even at low doses. Sex-related differences were noticed in all assessed parameters. While female rats eliminated AgNPs from the liver and kidneys more efficiently than males when treated with low doses, the opposite was observed for animals treated with higher doses of AgNPs. Female Wistar rats exposed to 1 mg PVP-coated AgNPs/kg b.w. accumulated two to three times more silver in the blood, liver, kidney and hearth than males, while the accumulation in most organs of digestive tract was more than ten times higher compared to males. Oxidative stress responses in the organs of males, except the liver of males treated with high doses, were less intense than in the organs of females. However, both Mt genes and Mt protein expression were significantly reduced after treatment in the liver and kidneys of males, while they remained unchanged in females. Conclusions Observed toxicity effects of AgNPs in Wistar rats revealed sex-related differences in response to an oral 28 days repeated exposure.

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