A label-free electrochemical biosensor based on screen-printed electrodes modified with gold nanoparticles for quick detection of bacterial pathogens

A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl4 and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). The resulting amperometric glucose biosensors was demonstrated to have a low detection limit (> 50 μM) and a wide linear range after optimization with AuNP electrodeposition.

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Competitive electrochemical biosensing of biotin using cadmium-modified titanium phosphate nanoparticles and 8-channel screen-printed disposable electrodes

Analytical Methods ◽

10.1039/c7ay00947j ◽

2017 ◽

Vol 9 (26) ◽

pp. 3983-3991 ◽

Cited By ~ 4

Author(s):

Daniel Martín-Yerga ◽

Javier Carrasco-Rodríguez ◽

Francisco J. García Alonso ◽

Agustín Costa-García

Keyword(s):

Electrochemical Biosensor ◽

Titanium Phosphate ◽

Electrochemical Biosensing ◽

Screen Printed Electrodes ◽

Screen Printed

Detection of biotin in multivitamin tablets was performed with a competitive electrochemical biosensor using cadmium-modified titanium phosphate nanoparticles and 8-channel screen-printed electrodes.

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A novel hybrid platform for the preparation of disposable enzyme biosensors based on poly(3,4-ethylenedioxythiophene) electrodeposition in an ionic liquid medium onto gold nanoparticles-modified screen-printed electrodes

Journal of Electroanalytical Chemistry ◽

10.1016/j.jelechem.2010.11.038 ◽

2011 ◽

Vol 656 (1-2) ◽

pp. 152-158 ◽

Cited By ~ 32

Author(s):

V. Serafín ◽

L. Agüí ◽

P. Yáñez-Sedeño ◽

J.M. Pingarrón

Keyword(s):

Gold Nanoparticles ◽

Ionic Liquid ◽

Liquid Medium ◽

Screen Printed Electrodes ◽

Hybrid Platform ◽

Ionic Liquid Medium ◽

Screen Printed

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Competitive amperometric immunosensor for determination of p53 protein in urine with carbon nanotubes/gold nanoparticles screen-printed electrodes: A potential rapid and noninvasive screening tool for early diagnosis of urinary tract carcinoma

Analytica Chimica Acta ◽

10.1016/j.aca.2017.09.005 ◽

2017 ◽

Vol 991 ◽

pp. 133-141 ◽

Cited By ~ 23

Author(s):

Marco Giannetto ◽

Maria Vittoria Bianchi ◽

Monica Mattarozzi ◽

Maria Careri

Keyword(s):

Carbon Nanotubes ◽

Gold Nanoparticles ◽

Urinary Tract ◽

Early Diagnosis ◽

Screening Tool ◽

P53 Protein ◽

Amperometric Immunosensor ◽

Screen Printed Electrodes ◽

Screen Printed

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Stable Electrochemical Measurements of Platinum Screen-Printed Electrodes Modified with Vertical ZnO Nanorods for Bacterial Detection

Journal of Nanomaterials ◽

10.1155/2019/2341268 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Thi Hong Phuoc Nguyen ◽

Matteo Tonezzer ◽

Thi Thanh Le Dang ◽

Quang Khue Vu ◽

Quang Huy Tran ◽

...

Keyword(s):

Electron Microscopy ◽

Salmonella Enteritidis ◽

Electrochemical Impedance ◽

Structural Characteristics ◽

Bacterial Pathogens ◽

Zno Nanorods ◽

Electrochemical Measurements ◽

Platinum Electrodes ◽

Screen Printed Electrodes ◽

Screen Printed

The study is aimed at investigating the stability of electrochemical and biosensing properties of ZnO nanorod-based platinum screen-printed electrodes (SPEs) applied for detection of bacterial pathogens. The platinum SPEs were designed and patterned according to standard photolithography and lift-off process on a silicon wafer. ZnO nanorods (NRs) were grown on the platinum working electrode by the hydrothermal method, whereas Salmonella polyclonal antibodies were selected and immobilized onto ZnO NR surface via a crosslinking process. Morphological and structural characteristics of ZnO NRs were investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The results showed that the ZnO NRs were grown vertically on platinum electrodes with a diameter around 20-200 nm and a length of 5-7 μm. These modified electrodes were applied for detection of Salmonella enteritidis at a concentration of 103 cfu/mL by electrochemical measurements including cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The ZnO NR-modified platinum electrodes could detect Salmonella bacteria well with stable measurements, and the signal to noise ratio was much higher than that of 3 : 1. This study indicated that ZnO NR-modified platinum SPEs could be potential for the development of biochips for electrochemical detection of bacterial pathogens.

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A carbon ink screen-printed immunoelectrode for Dengue virus NS1protein detection based on photosynthesized amine gold nanoparticles

Journal of Electronics and Sensors ◽

10.31829/2689-6958/jes2018-1(1)-102 ◽

2018 ◽

pp. 1-12

Keyword(s):

Gold Nanoparticles ◽

Dengue Virus ◽

Limit Of Detection ◽

Colloidal Suspension ◽

Electrochemical Immunosensor ◽

Label Free ◽

Vis Spectroscopy ◽

Label Free Detection ◽

The Impact ◽

Screen Printed

Dengue virus (DENV) is a reemerging mosquito-borne disease that is endemic in more than 125 countries, affecting 200 million people per year. Screening testing has been a good attempt to minimize the impact caused by high morbity and mortality rates of DENV. In this study, a simple and disposable label-free electrochemical immunosensor based on a carbon ink graphite screen-printed electrode (SPE) one-step fabricated was developed for detection of non-structural 1 protein (NS1). The SPE surface was modified by drop casting, depositing a colloidal suspension containing amine-functionalized gold nanoparticles (AuNP-NH2). AuNPs were synthetized by a photoinduced physical method, illuminating preformed gold seeds with a light-emitting diode (LED,) at blue region, by using the polyethyleneimine (NH2) as reductor and stabilizing agent. UV-VIS spectroscopy and Transmission Electron Microscopy (TEM) were used to characterize the amine AuNPs. Electrocatalytic activity of AuNPs allowed more sensitivity for a label-free detection of NS1 by square wave voltammetry (SWV), with linear response from 0.1 to 2 µg mL-1. It was found a good linearity (coefficient of correlation of 0.995 (p<0.01) and a limit of detection of 0.03 µg mL-1 NS1 for analytical responses. AuNP-NH2 synthesis provided an easy oriented immobilization of anti-NS1 antibodies by Fc portion, resulting in a simple fabrication immunosensor with relative high performance and feasibility for early diagnostic of DENV.

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