Molecular identification, phylogenetic analysis and antifungal susceptibility patterns of Aspergillus nidulans complex and Aspergillus terreus complex isolated from clinical specimens

ABSTRACT A phylogenetic analysis was performed for 34 Aspergillus strains belonging to section Nigri. Molecular methods allowed for the correct classification into three different clades (A. niger, A. tubingensis, and A. foetidus). Correlation with in vitro itraconazole susceptibility distinguished the following three profiles: susceptible, resistant, and showing a paradoxical effect. A number of different species whose morphological features resemble those of A. niger showed unusual MICs to itraconazole that have never been described for the Aspergillus genus.

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Characterization of clinical strains of Aspergillus terreus complex: molecular identification and antifungal susceptibility to azoles and amphotericin B

Clinical Microbiology and Infection ◽

10.1111/j.1469-0691.2011.03714.x ◽

2012 ◽

Vol 18 (2) ◽

pp. E24-E26 ◽

Cited By ~ 13

Author(s):

P. Escribano ◽

T. Peláez ◽

S. Recio ◽

E. Bouza ◽

J. Guinea

Keyword(s):

Amphotericin B ◽

Molecular Identification ◽

Aspergillus Terreus ◽

Antifungal Susceptibility ◽

Clinical Strains

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Molecular Identification and Antifungal Susceptibility Patterns of Candida Species Isolated from Candidemia Patients in Yasuj, Southwestern Iran

Jundishapur Journal of Microbiology ◽

10.5812/jjm.117643 ◽

2021 ◽

Vol 14 (7) ◽

Author(s):

Shaghayegh Rostami Yasuj ◽

Maral Gharaghani ◽

Seyed Sajjad Khoramrooz ◽

Marjan Salahi ◽

Ali Keshtkari ◽

...

Keyword(s):

Mortality Rate ◽

Amphotericin B ◽

Molecular Identification ◽

Candida Species ◽

Antifungal Susceptibility ◽

Hospitalized Patients ◽

Susceptibility Tests ◽

In Vitro Antifungal Susceptibility ◽

Susceptibility Patterns

Background: Candidemia is the most common systemic infection in hospitalized patients causing high mortality. Hence, the diagnosis of this infection in the early stage with appropriate antifungal therapy is paramount. Objectives: The study aimed at molecular identification of Candida species isolated from candidemia patients and evaluation of the in vitro antifungal susceptibility patterns of these strains to fluconazole, amphotericin B, and caspofungin. Methods: In the present study, 800 hospitalized patients who were suspected to have candidemia were sampled. Candida species were isolated and identified based on morphological characteristics and PCR-sequencing of the ITS1-5.8S-ITS2 region. Antifungal susceptibility tests for fluconazole, amphotericin B, and caspofungin were performed according to the Clinical and Laboratory Standards Institute protocol M27-A3. Also, clinical data were recorded from the patients' records. Results: Twenty-seven patients among the sample of hospitalized patients were found to have candidemia. A total of 33.3% of candidemia patients were treated with amphotericin B, in which case the mortality rate was 14.8%. The majority of patients (59%) were from the neonatal intensive care unit, and premature birth was the most common underlying condition. Candida albicans (n = 18; 66.6%) was the most common species isolated from blood cultures, followed by C. parapsilosis (n = 7; 25.9%), C. pelliculosa (n = 1; 3.7%), and C. tropicalis (n = 1; 3.7%). Only one C. albicans isolate resistant to fluconazole (minimum inhibitory concentration = 32 µg/mL). Conclusions: Generally, C. albicans has been the most frequent causative agent of candidemia. Resistance to antifungal drugs among candidemia agents was rare. Also, the identification of Candida isolates at the species level with in vitro antifungal susceptibility tests helps manage candidemia patients better and decrease the mortality rate among them.

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Molecular Identification and Antifungal Susceptibility Patterns of Clinical Dermatophytes Following CLSI and EUCAST Guidelines

Journal of Fungi ◽

10.3390/jof3020017 ◽

2017 ◽

Vol 3 (2) ◽

pp. 17 ◽

Cited By ~ 11

Author(s):

Yubhisha Dabas ◽

Immaculata Xess ◽

Gagandeep Singh ◽

Mragnayani Pandey ◽

Suneeta Meena

Keyword(s):

Molecular Identification ◽

Antifungal Susceptibility ◽

Susceptibility Patterns

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Species identification of Candida isolates in various clinical specimens and their antifungal susceptibility patterns in Cte dIvoire

African Journal of Microbiology Research ◽

10.5897/ajmr2015.7834 ◽

2016 ◽

Vol 10 (2) ◽

pp. 66-72 ◽

Cited By ~ 2

Author(s):

Andre Toure Offianan ◽

Ama Bonouma Ira ◽

Etienne Angora ◽

Bosson Henriette Vanga ◽

Kady Sylla ◽

...

Keyword(s):

Species Identification ◽

Antifungal Susceptibility ◽

Clinical Specimens ◽

Susceptibility Patterns

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CHARACTERISATION AND ANTIFUNGAL SUSCEPTIBILITY PATTERNS OF CANDIDA SPECIES ISOLATED IN TERTIARY CARE HOSPITAL IN NORTH INDIA.

10.36106/ijsr/9333492 ◽

2021 ◽

pp. 7-8

Author(s):

Meenakshi Sharma ◽

Chaudhary BL

Keyword(s):

Candida Albicans ◽

Candida Species ◽

Tertiary Care Hospital ◽

Antifungal Susceptibility ◽

Tertiary Care ◽

Diffusion Method ◽

Individual Species ◽

Care Hospital ◽

Clinical Specimens ◽

Susceptibility Patterns

Candida is usually considered a commensal with the potential to cause opportunistic infections ranging from supercial mucosal infections to lifethreatening invasive infections. This study was aimed to determine antifungal susceptibility patterns of Candida species isolated from various clinical specimens from a tertiary care hospital. Isolation and identic Material and Methods: ation of Candida species were done by morphological examination, pseudohyphae, and chlamydospore production on cornmeal agar, germ tube test, and HiCrome Candida differential agar. The antifungal susceptibility of Candida strains was performed by the disk diffusion method as per the CLSI M44-A2 protocol. A Result: total of 96 Candida species were isolated from various clinical specimens. The isolation rate was more in the older age group and female patients(52%). Most frequently isolated from urine 41.67% followed by sputum 19.79%, Blood 16.67%, and least from vaginal swab 2.08%. Four species of Candida were isolated among them Candida albicans was most common 59.37% followed by Candida prapsilosis and Candida krusei 15.62%, and Candida tropicalis 9.37%. In antifungal susceptibility testing, Itraconazole was the most sensitive drug and Fluconazole was the least sensitive one with only 58.33% susceptibility among individual species. Concussion: Urinary tract infection was common due to Candida albicans, and Itraconazole was the most susceptible drug.

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Molecular Identification, And Antifungal Susceptibility Patterns of Candida Species Isolated From Candidemia Patients in Yasuj, Southwestern Iran

10.21203/rs.3.rs-438157/v1 ◽

2021 ◽

Author(s):

Shaghayegh Rostami Yasuj ◽

Seyed Sajad Khoramrooz ◽

Marjan Salahi ◽

Ali Keshtkari ◽

Jabar Taghavi ◽

...

Keyword(s):

Mortality Rate ◽

Amphotericin B ◽

Molecular Identification ◽

Antifungal Susceptibility ◽

Hospitalized Patients ◽

Antifungal Drugs ◽

Its2 Region ◽

Susceptibility Tests ◽

Susceptibility Patterns

Abstract Candidemia is the most common systemic infection in hospitalized patients and causing high mortality. Hence, the diagnosis of this infection in the early-stage with appropriate antifungal therapy has been attributed to the lowest mortality. The aims of this study are molecular identification of Candida species isolated from candidemia patients and evaluated the in vitro antifungal susceptibility patterns of these strains to fluconazole, amphotericin B, and caspofungin. In the present study, 800 hospitalized patients who suspected candidemia were sampled. Candida species were isolated and identified based on morphological and PCR-sequencing of the ITS1-5.8S-ITS2 region. Antifungal susceptibility tests for fluconazole, amphotericin B, and caspofungin was performed according to the Clinical and Laboratory Standards Institute M27-A3. Also, clinical data were recorded from patient's records. Overall, 27 candidemia patients were detected among hospitalized patients. 33.3% of candidemia patients were treated with amphotericin B, however, the mortality rate was 14.8%. The majority of patients (59%) were from the neonatal intensive care unit and premature born was the most underlying condition. C. albicans (n = 18; 66.6%) was the most common species isolated from blood cultures, followed by C.parapsilosis (n = 7; 25.9%), C.pelliculosa ( n = 1;3.7% ) and C.tropicalis (n = 1;3.7%). Only one C. albicans isolate were resistance to fluconazole (MIC = 32 µg/mL). Generally, C. albicans has been the most frequent causative agent of candidemia. Resistance to antifungal drugs among candidemia agents was rare. Also, the identification of Candida isolates at the species level with in-vitro antifungal susceptibility tests can manage and decrease the mortality rate among candidemia patients.

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