Anthocyanin complex niosome gel accelerates oral wound healing: in vitro and clinical studies

2021 ◽  
pp. 102423
Author(s):  
Teerasak Damrongrungruang ◽  
Jarin Paphangkorakit ◽  
Sucharat Limsitthichaikoon ◽  
Bhattaranitch Khampaenjiraroch ◽  
Michael Jonathan Davies ◽  
...  
Keyword(s):  
Wound Healing ◽  
Clinical Studies ◽  
Oral Wound Healing

scholarly journals Dexpanthenol in Wound Healing after Medical and Cosmetic Interventions (Postprocedure Wound Healing)

2020 ◽  
Vol 13 (7) ◽  
pp. 138
Author(s):  
Julian Gorski ◽  
Ehrhardt Proksch ◽  
Jens Malte Baron ◽  
Daphne Schmid ◽  
Lei Zhang
Keyword(s):  
Wound Healing ◽  
Clinical Studies ◽  
New Technologies ◽  
Healing Process ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Skin Damage ◽  
Published Evidence ◽  
Superficial Skin

With the availability of new technologies, the number of subjects undergoing medical and cosmetic interventions is increasing. Many procedures (e.g., ablative fractional laser treatment) resulting in superficial/minor wounds require appropriate aftercare to prevent complications in wound healing and poor cosmetic outcome. We review the published evidence of the usefulness of topical dexpanthenol in postprocedure wound healing and the associated mechanisms of action at the molecular level. A search in the PubMed and Embase databases was performed to query the terms dexpanthenol, panthenol, superficial wound, minor wound, wound healing, skin repair, and postprocedure. Search results were categorized as clinical trials and in vitro studies. In vitro and clinical studies provided evidence that topically applied dexpanthenol promotes superficial and postprocedure wound healing. Latest findings confirmed that dexpanthenol upregulates genes that are critical for the healing process. The gene expression data are of clinical relevance as evidenced by prospective clinical studies indicating that topical dexpanthenol accelerates wound healing with rapid re-epithelialization and restoration of skin barrier function following skin injury. It can therefore be inferred that topical dexpanthenol represents an appropriate and state-of-the-art treatment option for superficial postprocedure wounds, especially when applied early after the superficial skin damage.

Activation of latent TGF-β1 by low-power laser in vitro correlates with increased TGF-β1 levels in laser-enhanced oral wound healing

2007 ◽  
Vol 15 (6) ◽  
pp. 866-874 ◽  
Author(s):  
Praveen R. Arany ◽  
Ramakant S. Nayak ◽  
Seema Hallikerimath ◽  
Anil M. Limaye ◽  
Alka D. Kale ◽  
...  
Keyword(s):  
Wound Healing ◽  
Low Power ◽  
Power Laser ◽  
Oral Wound Healing ◽  
Tgf Β1

scholarly journals Evaluation of the In Vitro Oral Wound Healing Effects of Pomegranate (Punica granatum) Rind Extract and Punicalagin, in Combination with Zn (II)

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1234 ◽  
Author(s):  
Vildan Celiksoy ◽  
Rachael L. Moses ◽  
Alastair J. Sloan ◽  
Ryan Moseley ◽  
Charles M. Heard
Keyword(s):  
Wound Healing ◽  
Antioxidant Activities ◽  
Punica Granatum ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Anti Inflammatory ◽  
High Concentrations ◽  
And Migration ◽  
Oral Wound Healing

Pomegranate (Punica granatum) is a well-established folklore medicine, demonstrating benefits in treating numerous conditions partly due to its antimicrobial and anti-inflammatory properties. Such desirable medicinal capabilities are attributed to a high hydrolysable tannin content, especially punicalagin. However, few studies have evaluated the abilities of pomegranate to promote oral healing, during situations such as periodontal disease or trauma. Therefore, this study evaluated the antioxidant and in vitro gingival wound healing effects of pomegranate rind extract (PRE) and punicalagin, alone and in combination with Zn (II). In vitro antioxidant activities were studied using DPPH and ABTS assays, with total PRE phenolic content measured by Folin–Ciocalteu assay. PRE, punicalagin and Zn (II) combination effects on human gingival fibroblast viability/proliferation and migration were investigated by MTT assay and scratch wounds, respectively. Punicalagin demonstrated superior antioxidant capacities to PRE, although Zn (II) exerted no additional influences. PRE, punicalagin and Zn (II) reduced gingival fibroblast viability and migration at high concentrations, but retained viability at lower concentrations without Zn (II). Fibroblast speed and distance travelled during migration were also enhanced by punicalagin with Zn (II) at low concentrations. Therefore, punicalagin in combination with Zn (II) may promote certain anti-inflammatory and fibroblast responses to aid oral healing.

scholarly journals Human saliva stimulates skin and oral wound healing in vitro

2019 ◽  
Vol 13 (6) ◽  
pp. 1079-1092 ◽  
Author(s):  
Charlotte Rodrigues Neves ◽  
Jeroen Buskermolen ◽  
Sanne Roffel ◽  
Taco Waaijman ◽  
Maria Thon ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Saliva ◽  
Oral Wound Healing

Topical oral wound healing potential of anthocyanin complex: animal and clinical studies

2018 ◽  
Vol 9 (5) ◽  
pp. 359-374 ◽  
Author(s):  
Sucharat Limsitthichaikoon ◽  
Bhattaranitch Khampaenjiraroch ◽  
Teerasak Damrongrungruang ◽  
Wanwisa Limphirat ◽  
Suthasinee Thapphasaraphong ◽  
...  
Keyword(s):  
Wound Healing ◽  
Clinical Studies ◽  
Oral Wound Healing

Aberrant mucosal wound repair in the absence of secretory leukocyte protease inhibitor

2004 ◽  
Vol 92 (08) ◽  
pp. 288-297 ◽  
Author(s):  
Moon-Jin Jeong ◽  
Salvador Nares ◽  
Gillian Ashcroft ◽  
Nikola Angelov ◽  
Niki Moutsopoulos ◽  
...  
Keyword(s):  
Wound Healing ◽  
Protease Inhibitor ◽  
Wound Repair ◽  
Molecular Mechanisms ◽  
Secretory Leukocyte Protease Inhibitor ◽  
Matrix Deposition ◽  
Wound Model ◽  
Novel Approach ◽  
Oral Wound Healing

SummarySecretory leukocyte protease inhibitor (SLPI) is a cationic serine protease inhibitor with anti-microbial and anti-inflammatory properties found in large quantities in mucosal fluids, including saliva. SLPI is expressed during cutaneous wound healing, however, its role in oral wound repair is unknown. We have used a novel approach involving a murine buccal mucosal acute wound model to investigate the role of SLPI in oral healing. In parallel to the observed cutaneous healing phenotype, an absence of SLPI results in markedly impaired oral wound healing associated with increased inflammation and raised elastase activity. Moreover, matrix deposition was decreased, while MMP activity was enhanced in the oral SLPI null wounds suggesting deregulated proteolysis. Intriguingly, regardless of genotype, reduced collagen deposition was observed in oral compared to dermal wounds, associated with reduced TGF-β expression and decreased fibroblast collagen expression in vitro. We propose that SLPI is a pivotal endogenous factor necessary for optimal tissue repair including intra-oral wound healing. In addition, our model provides a unique opportunity to delineate the cellular and molecular mechanisms underlying the differences between dermal scarring and oral scar-free healing.

scholarly journals Cytocompatibility Properties of an Herbal Compound Solution Support In vitro Wound Healing

2021 ◽  
Vol 12 ◽  
Author(s):  
Peng Zhou ◽  
Vanessa Chrepa ◽  
Ioannis Karoussis ◽  
Michael A. Pikos ◽  
Georgios A. Kotsakis
Keyword(s):  
Wound Healing ◽  
Herbal Extract ◽  
Active Comparator ◽  
Cellular Models ◽  
Fibroblast Migration ◽  
Oral Rinse ◽  
Apical Papilla ◽  
Oral Stem Cells ◽  
Oral Wound Healing

The aim of this study was to evaluate the cytocompatibility of an herbal extract compound oral rinse [StellaLife VEGA (SLife)] against relevant human cellular models of oral surgical wound healing. SL was compared to the gold standard for peri-/post-operative oral surgical use, i.e., Chlorhexidine (CHX) and to a commonly utilized essential-oil (EO) based antiseptic rinse. Fibroblasts and primary oral stem cells of the apical papilla (SCAPs) were employed to assess its comparative cytotoxicity to the active comparator antiseptic rinses and its effects on wound healing in vitro. In cytotoxicity assays, multiple timepoints were tested ranging from clinically relevant of 60-s rinsing to protracted challenge of up to 5 min, to determine dose-dependent toxicity. The SLife group consistently demonstrated minimal cytotoxicity as compared to active comparators across experimental timepoints and different cells lines. At concentrations up to 20% v/v SLife-challenged fibroblasts and SCAPs demonstrated no significant toxicity as compared to unstimulated controls (p > 0.05). When assessing wound healing, a scratch wound assay revealed significantly accelerated cell migration for SLife as compared to CHX (p < 0.05). Notably, all active comparator antiseptic rinses affected wound healing responses by significantly reducing total collagen deposition after intermittent “rinsing” intervals that simulated post-surgical oral rinsing. Nonetheless, intermittent as well as continuous challenge of cells with SLife had a positive effect in functional collagen assays. An herbal extract compound-based oral rinse was found to be cytocompatible to cells critical to oral wound healing and to promote fibroblast migration and differentiation, contrary to existing antiseptic rinses that lack selective cytotoxicity.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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