Wound healing potentials of a herbal homeopathic remedy on NIH 3T3 fibroblasts in vitro

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
K Hostanska ◽  
M Rostock ◽  
R Saller
Keyword(s):  
Wound Healing ◽  
Homeopathic Remedy ◽  
3T3 Fibroblasts ◽  
Nih 3T3

scholarly journals Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Sara H. Freiesleben ◽  
Jens Soelberg ◽  
Nils T. Nyberg ◽  
Anna K. Jäger
Keyword(s):  
Wound Healing ◽  
Medicinal Plants ◽  
Plant Species ◽  
Cold Water ◽  
Water Extract ◽  
Scratch Assay ◽  
3T3 Fibroblasts ◽  
Nih 3T3 ◽  
Wound Healing Activity

The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice. The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species. HPLC separation of the most active plant extract, which was a warm water extract of Philenoptera cyanescens, revealed the wound healing activity to be attributed to rutin and a triglycoside of quercetin. The present study suggests that Allophylus spicatus, Philenoptera cyanescens, Melanthera scandens, Ocimum gratissimum, and Jasminum dichotomum have wound healing activity in vitro.

scholarly journals Effect of two homeopathic remedies at different degrees of dilutions on the wound closure of 3T3 fibroblasts in in vitro scratch assay

2021 ◽  
Vol 11 (40) ◽  
pp. 164-165
Author(s):  
Katarina Hostanska ◽  
Matthias Rostock ◽  
Stephan Baumgartner ◽  
Reinhard Saller
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Wound Closure ◽  
Hypericum Perforatum ◽  
Calendula Officinalis ◽  
Arnica Montana ◽  
Fibroblast Migration ◽  
3T3 Fibroblasts ◽  
Nih 3T3

Background: Since ancient times, preparations from traditional medicinal plants e.g. Arnica montana, Calendula officinalis or Hypericum perforatum have been used for different wound healing purposes. The aim of this study was to investigate the efficacy of the commercial low dilution homeopathic remedy Similasan® Arnica plus Spray, a preparation of Arnica montana 4x, Calendula officinalis 4x, Hypericum perforatum 4x and Symphytum officinale 6x (0712-2) and medium diluted SIM WuS (Petroleum 15x, Arnica montana 15x, Calcium fluoratum 12x, Calendula officinalis 12x, Hepar sulfuris 12x and Mercurius solubilis 15x; 1101-4), on the wound healing in cultured NIH 3T3 fibroblasts. Both remedies were from Similasan AG (Jonen, Switzerland) and prepared according the German Homoeopathic Pharmacopoeia (GHP) following descriptions 4a for arnica, 3a for marigold and St. John’s wort, 2a for comfrey, 5a for petroleum, and 6 for calcium fluoride, hepar sulfuris and mercurius solubilis. Materials and Methods: Cell proliferation, migration and wound closure promoting effect of the preparations (0712-2, 1101- 4) and their succussed solvents (0712-1, 1101-3) were investigated on mouse NIH 3T3 fibroblasts. Cell viability was determined by WST-1 assay, cell growth using BrdU uptake, cell migration by chemotaxis assay and wound closure by CytoSelect ™Wound Healing Assay Kit which generated a defined wound area. All assays were performed in three independent controlled experiments. In some experiments diluted unsuccussed alcohol (0712-3) was also investigated. Results: Preparations (0712-1), (0712-2), (0712-3), (1101-3) and (1101-4) were investigated at decimal dilution steps from 1x to 4x. Cell viabilty was not affected by any of the substances and (0712-1) and (0712-2) showed no stimulating effect on cell proliferation. Preparation (0712-2) exerted a stimulating effect on fibroblast migration (31.7%) vs 15% with succussed solvent (0712-1) at 1:100 dilutions (p0.05). Positive control 2 ng/ml EGF increased migratory activity of cells by 49.8%. Preparation (0712-2) at a dilution of 1:100 promoted in vitro wound closure by 59.5% and differed significantly (p

scholarly journals Effects of Lipophilic Extract ofViscum albumL. and Oleanolic Acid on Migratory Activity of NIH/3T3 Fibroblasts and on HaCat Keratinocytes

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
R. Kuonen ◽  
U. Weissenstein ◽  
K. Urech ◽  
M. Kunz ◽  
K. Hostanska ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oleanolic Acid ◽  
Wound Closure ◽  
Viscum Album ◽  
Hacat Keratinocytes ◽  
Lipophilic Extract ◽  
3T3 Fibroblasts ◽  
Nih 3T3

Viscum albumL. lipophilic extract (VALE) contains pharmacologically active pentacyclic triterpenes that are known to exhibit immunomodulatory, antitumor, and wound healing activity. Preliminary clinical observations indicate that VALE was able to influence cutaneous wound healingin vivo. The objective of this study was to investigate wound closure related properties of VALEin vitro. As measured in a wound healing assay, VALE and its predominant triterpene oleanolic acid (OA) significantly and dose dependently promoted the migration of NIH/3T3 fibroblastsin vitro, thereby leading to an enhanced wound closure. Compared to the negative control, maximal stimulation by 26.1% and 26.2%, respectively, was attained with 10 μg/mL VALE and 1 μg/mL OA. Stimulation of proliferation in NIH/3T3 fibroblasts by VALE and OA could be excluded. At higher concentrations both substances affected proliferation and viability of NIH/3T3 fibroblasts and HaCat keratinocytes. In the toxic range of concentrations of VALE and OA, migration of NIH/3T3 fibroblasts was suppressed. The extent of the stimulatory effect on cell migration of VALE quite closely corresponded to the effect expected by the concentrations of OA contained in the crude extract VALE. These data support the casual observation thatViscum albumL. lipophilic extract might modulate wound healing related processesin vivo.

scholarly journals In VitroAntileukemic Activity ofXanthosoma sagittifolium(Taioba) Leaf Extract

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Marina L. C. Caxito ◽  
Rachell R. Correia ◽  
Anne Caroline C. Gomes ◽  
Graça Justo ◽  
Marsen G. P. Coelho ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Leaf Extract ◽  
Leukemia Cells ◽  
No Production ◽  
3T3 Fibroblasts ◽  
Griess Reaction ◽  
Xanthosoma Sagittifolium ◽  
M Phase ◽  
Nih 3T3

Xanthosoma sagittifoliumSchott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity ofX. sagittifoliumleaf extract. Results showed that hydroethanolic extract ofX. sagittifoliumleaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract ofXanthosoma sagittifoliumpresented chelating activity andin vitroantitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

scholarly journals Development of a Novel Film Based on Bacterial Nanocellulose Reinforced Gelatin/Guar Gum Containing Honey for Wound Healing Applications

2021 ◽  
Author(s):  
Parinaz Nezhadmokhtari ◽  
Nahideh Asadi ◽  
Marjan Ghorbani ◽  
Azizeh Rahmani Del Bakhshayesh ◽  
Morteza Milani ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Water Solubility ◽  
Guar Gum ◽  
Structural Features ◽  
Polymer Gel ◽  
Bacterial Nanocellulose ◽  
Nih 3T3 ◽  
Cell Adhesion And Proliferation

Abstract Bacterial nanocellulose (BNC) is a type of 3-dimensionally structured polymer gel produced by Acetobacter that has recently attracted increased interest in wound healing concerns. To produce an effective antibacterial wound dressing, researchers investigated the manufacturing and structural features of honey-infused BNC reinforced gelatin/aldehyde-modified Guar gum films (H/BNC/Ge/AD-GG). Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), mechanical characteristics, water solubility, and degradability were all used to assess the produced films. In addition, the influence of honey addition on the produced films' various properties has been examined. Antibacterial activity, better degradation capability, improved mechanical qualities, and excellent cell adhesion and proliferation by NIH-3T3 fibroblast cells were among the outcomes. The cytotoxicity assay in vitro revealed good cytocompatibility. As a result of the findings, the produced H/BNC/Ge/AD-GG films appear to have a high potential for antibacterial wound dressing applications.

scholarly journals The Activity of Combination of Hydrolyzed Virgin Coconut Oil and Chitosan Toward Wound Healing Parameters on NIH 3T3 Cells Using in Vitro Methods

1970 ◽  
Vol 7 (3) ◽  
pp. 14-19 ◽  
Author(s):  
Hekdin Marsius Sipayung ◽  
Jansen Silalahi ◽  
Yuandani Y
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Protein Expression ◽  
Scratch Wound ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Cox 2 ◽  
Nih 3T3 ◽  
Vegf Protein

Objectives: The objective of this study was to investigate the activity of combination of hydrolyzed VCO (HVCO) and chitosan on NIH 3T3 cell proliferation activity, NIH 3T3 cell migration, COX-2 and VEGF protein expression. Design: In vitro cytotoxic assay was determined by MTT (MicrocultureTetrazoliumTehnique) assay, cell proliferation activity was measured by calculating cell viability incubated 24 hours, 48 hours and 72 hours, wound closure percentage was tested by scratch wound healing method, expression of COX-2 protein and VEGF protein were measured by immunocytochemical method. Interventions: The variable that was intervened in this study was the concentration of HVCO and chitosan. Main Outcome Measures: The main measurements carried out in this study were the absorbance value of HVCO and chitosan which was converted into viability cell, proliferation activity, percentage of wound closure, and percentage of COX-2 and VEGF protein expression. Results: Cytotoxic activity of HVCO and chitosan resulted the best concentration at 31.25 μg/ml, scratch wound healing assay from a combination HVCO and chitosan resulted the best migration of fibroblast cells at a ratio of 1:1 with HVCO 62.5 μg/ml and chitosan 62.5 μg/ml, combination of HVCO 62.5 μg/ml and chitosan 62.5 μg/ml (1:1) increased expression of COX-2 and VEGF. Conclusion: Combination of HVCO and chitosan could increase NIH 3T3 cell migration, COX-2 and VEGF protein expression. Combination of HVCO and chitosan had better wound healing activity in vitro than single use. Keywords: Rhizomucor miehei, viability, proliferation, migration, expression

scholarly journals Effect of heat treatment on cytotoxicity of self-adhesive resin cements: Cell viability analysis

2018 ◽  
Vol 12 (02) ◽  
pp. 281-286 ◽  
Author(s):  
Celso Afonso Klein-Júnior ◽  
Roberto Zimmer ◽  
Guilherme Scotta Hentschke ◽  
Denise Cantarelli Machado ◽  
Rubem Beraldo dos Santos ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Cell Viability ◽  
Fibroblast Cell ◽  
Resin Cements ◽  
Adhesive Resin ◽  
Viability Analysis ◽  
3T3 Fibroblasts ◽  
Mixing Control ◽  
Nih 3T3

ABSTRACT Objective: The aim of the study was to assess, in vitro, the influence on cytotoxicity of heat treatment applied before photopolymerization, while mixing three self-adhesive resin cements, in an NIH/3T3 fibroblast cell culture, based on cell viability measures. Methods: Samples were divided into three groups: (1) no heat treatment while mixing (control), (2) 37°C, and (3) 60°C heat treatment while mixing. Cements were light-cured immediately after mixing and immersed in Dulbecco's Modified Eagle Media for the extraction of possibly uncured products after 24 h and 7 days. Cultures contained 0.5 mL of NIH/3T3 fibroblasts per well at a concentration of 0.4 × 105 cells/mL and specific extracts for each sample. Statistical Analysis Used: Data were statistically analyzed with ANOVA and post hoc Student–Newman–Keuls (significance of 5%). Results: Cement cytotoxicity increased with time, as shown by the higher values observed at 7 days. There was a slight difference in intragroup cytotoxicity levels between 24 h and 7 days. Heat treatment at 60°C was associated with a major decrease in cytotoxicity levels in all three groups, both at 24 h and at 7 days, with no differences among the cements. Conclusions: Heat treatment at 60°C should be considered as a strategy to reduce cytotoxicity of self-adhesive resin cements, as evidenced by the results observed at 24 h and 7 days of analysis.

In-vitro Wound Healing Properties of Commiphora swynnertonii Resinous Extracts

2021 ◽  
Vol 38 ◽  
pp. 32-37
Author(s):  
G.G. Bakari ◽  
S.A. Mshamu ◽  
M.H. Ally ◽  
R.A. Max ◽  
H. Bai
Keyword(s):  
Wound Healing ◽  
Herbal Remedies ◽  
3T3 Cells ◽  
Scratch Assay ◽  
Sequence Of Events ◽  
Nih 3T3 ◽  
Wound Scratch Assay ◽  
Commiphora Swynnertonii ◽  
Nih 3T3 Cells

Wound healing is a complex multicellular process involving many cell types which include; inflammatory cells, endothelial cells, fibroblasts and keratinocytes. The process involves an orderly sequence of events with four overlapping phases namely; haemostasis, inflammatory, proliferation and remodeling phases.  The process can be facilitated by the use of wound healing agents including herbal remedies from plants. In this study the main objective was to evaluate the in vitro wound healing activity of the resin obtained from Commiphora swynnertonii (C.swynnertonii). First the NIH -3T3 cells viability were evaluated using (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl Tetrazolium Bromide (MTT) assay. Then the wound scratch assay model was used to evaluate cellular proliferation, closure of the wound and release of matrix metalloproteinase enzymes. Results indicate differences in mean cell viability between different concentrations within 24 hours of incubation. The highest viability was recorded at the concentration of 1% (v/v). The in-vitro wound scratch assay showed positive NIH - 3T3 cells proliferation on the wound area and cells migration when compared with control group (without treatment) at 0 and 24 hours. In addition, C. swynnertonii was able to stimulate secretion of MMP-2 release from NIH - 3T3 cells. MMP-2 is an important enzyme for extracellular matrix remodeling during wound healing suggesting that C. swynnertonii promotes wound healing by stimulating cell proliferation and production of MMP-2 in a mechanism that is currently not known.

scholarly journals Efficacy Study of Broken Rice Maltodextrin inIn VitroWound Healing Assay

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Zahiah Mohamed Amin ◽  
Soo Peng Koh ◽  
Swee Keong Yeap ◽  
Nur Syazwani Abdul Hamid ◽  
Chin Ping Tan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Delivery Systems ◽  
Efficacy Study ◽  
Dextrose Equivalent ◽  
Broken Rice ◽  
Nih 3T3 ◽  
Better Than

Maltodextrins that contain both simple sugars and polymers of saccharides have been widely used as ingredients in food products and pharmaceutical delivery systems. To date, no much work has been reported on the applications of maltodextrin from broken rice (RB) sources. Therefore, the objective of this work was to investigate thein vitrowound healing efficacy of RB maltodextrin at different conditions. Wounds treated with lower dextrose equivalent (DE) range (DE 10–14) of maltodextrins at a concentration of 10% obtained from RB were found to be able to heal the wounds significantly faster (p<0.01) than maltodextrin with higher DE ranges (DE 15–19 and DE 20–24) and concentrations of 5% and 20%. The findings from both BrdU and MTT assay further confirmed its wound healing properties as the NIH 3T3 fibroblast wounded cells were able to proliferate without causing cytotoxic effect when wounded cell was treated with maltodextrin. All these findings indicated that the RB maltodextrin could perform better than the commercial maltodextrin at the same DE range. This study showed that RB maltodextrins had better functionality properties than other maltodextrin sources and played a beneficial role in wound healing application.

Sign in / Sign up

Export Citation Format

Share Document