INCREASE: An in-cell reaction system for multi-nucleon transfer and spontaneous fission at the FRS ion catcher

2022 ◽  
Vol 512 ◽  
pp. 83-90
Author(s):  
Adrian Rotaru ◽  
Daler Amanbayev ◽  
Dimiter L. Balabanski ◽  
David Benyamin ◽  
Paul Constantin ◽  
...  
Keyword(s):  
Spontaneous Fission ◽  
Reaction System ◽  
Cell Reaction ◽  
Nucleon Transfer

scholarly journals P212A Mutant of Dihydrodaidzein Reductase Enhances (S)-Equol Production and Enantioselectivity in a Recombinant Escherichia coli Whole-Cell Reaction System

2016 ◽  
Vol 82 (7) ◽  
pp. 1992-2002 ◽  
Author(s):  
Pyung-Gang Lee ◽  
Joonwon Kim ◽  
Eun-Jung Kim ◽  
EunOk Jung ◽  
Bishnu Prasad Pandey ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Reaction System ◽  
Gut Bacteria ◽  
Site Directed Mutagenesis ◽  
Anaerobic Growth ◽  
Efficient Production ◽  
Cell Reaction ◽  
Whole Cell ◽  
Content Type ◽  
Postmenopausal Symptoms

ABSTRACT(S)-Equol, a gut bacterial isoflavone derivative, has drawn great attention because of its potent use for relieving female postmenopausal symptoms and preventing prostate cancer. Previous studies have reported on the dietary isoflavone metabolism of several human gut bacteria and the involved enzymes for conversion of daidzein to (S)-equol. However, the anaerobic growth conditions required by the gut bacteria and the low productivity and yield of (S)-equol limit its efficient production using only natural gut bacteria. In this study, the low (S)-equol biosynthesis of gut microorganisms was overcome by cloning the four enzymes involved in the biosynthesis fromSlackia isoflavoniconvertensintoEscherichia coliBL21(DE3). The reaction conditions were optimized for (S)-equol production from the recombinant strain, and this recombinant system enabled the efficient conversion of 200 μM and 1 mM daidzein to (S)-equol under aerobic conditions, achieving yields of 95% and 85%, respectively. Since the biosynthesis oftrans-tetrahydrodaidzein was found to be a rate-determining step for (S)-equol production, dihydrodaidzein reductase (DHDR) was subjected to rational site-directed mutagenesis. The introduction of the DHDR P212A mutation increased the (S)-equol productivity from 59.0 mg/liter/h to 69.8 mg/liter/h in the whole-cell reaction. The P212A mutation caused an increase in the (S)-dihydrodaidzein enantioselectivity by decreasing the overall activity of DHDR, resulting in undetectable activity for (R)-dihydrodaidzein, such that a combination of the DHDR P212A mutant with dihydrodaidzein racemase enabled the production of (3S,4R)-tetrahydrodaidzein with an enantioselectivity of >99%.

Allograft rejection in the mixed cell reaction system of the demosponge Suberites domuncula is controlled by differential expression of apoptotic genes

2004 ◽  
Vol 56 (8) ◽  
pp. 597-610 ◽  
Author(s):  
Matthias Wiens ◽  
Sanja Perović-Ottstadt ◽  
Isabel M. Müller ◽  
Werner E. G. Müller
Keyword(s):  
Differential Expression ◽  
Allograft Rejection ◽  
Reaction System ◽  
Cell Reaction ◽  
Mixed Cell ◽  
Suberites Domuncula ◽  
Apoptotic Genes

NUCLEON TRANSFER TO CONTINUUM STATES

1987 ◽  
Vol 48 (C2) ◽  
pp. C2-265-C2-270
Author(s):  
A. BONACCORSO ◽  
D. M. BRINK
Keyword(s):  
Nucleon Transfer ◽  
Continuum States

PENGGUNAAN AIR SUPER KRITIS PADA DEPOLIMERISASI NYLON-12 (BATCH PROCESS)

2018 ◽  
Vol 5 (3) ◽  
Author(s):  
Mohamad Yusman
Keyword(s):  
Supercritical Water ◽  
Scale Up ◽  
Experimental Studies ◽  
Reaction System ◽  
Batch Process ◽  
Chemical Recycling ◽  
Water Decomposition ◽  
Product Distributions ◽  
New Process ◽  
Nylon 12

Water at the supercritical state is a new process for the chemical recycling. At this thermodynamic state i.e. Pc = 218 atmospheres and Tc = 374oC , water behaves very differently from its everyday temperament and it is a very good solvent for organic components. Experimental studies show that supercritical water can decompose hydrocarbons/polymers and produce useful products like 2-Azacyclotridecanone /lactam-1 from Nylon-12 (batch process). The decomposition process itself was carried out in batch reaction system in order to get more information about product distributions, time dependence, and scale-up possibilities.Keywords: supercritical water, decomposition, batch, polymer, hydrocarbon

Optimization for ISSR-PCR reaction system of tea plant

2010 ◽  
Vol 36 (4) ◽  
pp. 414-417
Author(s):  
Jing NING ◽  
Jian-an HUANG ◽  
Juan LI ◽  
Xing-gang ZHONG ◽  
Qi ZHU
Keyword(s):  
Reaction System ◽  
Tea Plant ◽  
Issr Pcr

Establishment and optimization of ISSR-PCR reaction system for endophytic fungi of Huperzia crispata

2013 ◽  
Vol 38 (4) ◽  
pp. 398-403
Author(s):  
Yun-feng SHI ◽  
Li-jun YU ◽  
Zhong-hua LIU ◽  
Huang HUANG ◽  
Jin-hua XU ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Reaction System ◽  
Issr Pcr

Polymer-Bound Triphenylphosphine and 4,4′-Dinitroazobenzene as a Coupling Reagents for Chromatography-Free Esterification Reaction

2019 ◽  
Vol 16 (7) ◽  
pp. 1024-1031
Author(s):  
Diparjun Das ◽  
Kalyani Rajkumari ◽  
Lalthazuala Rokhum
Keyword(s):  
Microwave Irradiation ◽  
Solid Phase ◽  
Reaction System ◽  
Triphenylphosphine Oxide ◽  
High Yield ◽  
Sustainable Chemistry ◽  
Esterification Reaction ◽  
Coupling Reagent ◽  
Simple Filtration ◽  
Esterification Reactions

Aim and Objective: Sustainable production of fine chemicals both in industries and pharmaceuticals heavily depends on the application of solid-phase synthesis route coupled with microwave technologies due to their environmentally benign nature. In this report, a microwave-assisted esterification reaction using polymer-bound triphenylphosphine and 4,4′-dinitroazobenzene reagent system was investigated. Materials and Methods: The solvents were obtained from Merck India. Polymer-bound triphenylphosphine (~3 mmol triphenylphosphine moiety/g) was acquired from Sigma-Aldrich. The progress of the reaction was observed by thin-layer chromatography. All the reactions were performed in Milestones StartSYNTH microwave. The NMR spectra were recorded on Bruker Avance III 300, 400, and 500 MHz FT NMR Spectrometers. Using azo compound and polymer-bound triphenyl phosphine as a coupling reagent, esterification of different carboxylic acids with alcohols was performed under microwave irradiation. Results: Esterification of benzoic acid with 1-propanol under microwave irradiation gave a high yield of 92% propyl benzoate in 60 minutes only. Isolation of the ester products was relatively simple as both the byproducts polymer-bound triphenylphosphine oxide and hydrazine could be removed by simple filtration. The rates of reactions were found to be directly proportional to the pKa of the benzoic acids. Conclusion: 4,4′-Dinitroazobenzene was introduced as a novel coupling reagent, in conjugation with polymer-bound triphenylphosphine, for esterification reactions under microwave irradiation. The low moisture sensitivity of the reaction system, easy separation of the byproducts, and column chromatographyfree isolation of esters help our methods with application significance, particularly from the ‘Sustainable Chemistry’ perspective.

Kinetics of the catalytic hydrodesulphurization reaction system; hydrogenolysis of thiophene

1980 ◽  
Vol 45 (10) ◽  
pp. 2728-2741 ◽  
Author(s):  
Pavel Fott ◽  
Petr Schneider
Keyword(s):  
Atmospheric Pressure ◽  
Active Sites ◽  
Flow Reactor ◽  
Kinetic Equations ◽  
Reaction System ◽  
Reaction Products ◽  
Molybdenum Catalyst ◽  
Cobalt Molybdenum Catalyst ◽  
Kinetics Of ◽  
Reaction Schemes

Kinetics have been studied of the reaction system taking place during the reaction of thiophene on the cobalt-molybdenum catalyst in a gradientless circulation flow reactor at 360 °C and atmospheric pressure. Butane has been found present in a small amount in the reaction products even at very low conversion. In view of this, consecutive and parallel-consecutive (triangular) reaction schemes have been proposed. In the former scheme the appearance of butane is accounted for by rate of desorption of butene being comparable with the rate of its hydrogenation. According to the latter scheme part of the butane originates from thiophene via a different route than through hydrogenation of butene. Analysis of the kinetic data has revealed that the reaction of thiophene should be considered to take place on other active sites than that of butene. Kinetic equations derived on this assumption for the consecutive and the triangular reaction schemes correlate experimental data with acceptable accuracy.

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