Allograft rejection in the mixed cell reaction system of the demosponge Suberites domuncula is controlled by differential expression of apoptotic genes

2004 ◽  
Vol 56 (8) ◽  
pp. 597-610 ◽  
Author(s):  
Matthias Wiens ◽  
Sanja Perović-Ottstadt ◽  
Isabel M. Müller ◽  
Werner E. G. Müller
Keyword(s):  
Differential Expression ◽  
Allograft Rejection ◽  
Reaction System ◽  
Cell Reaction ◽  
Mixed Cell ◽  
Suberites Domuncula ◽  
Apoptotic Genes

Evolution of gene expression patterns in a model of branching morphogenesis

1999 ◽  
Vol 277 (4) ◽  
pp. F650-F663 ◽  
Author(s):  
Anna Pavlova ◽  
Robert O. Stuart ◽  
Martin Pohl ◽  
Sanjay K. Nigam
Keyword(s):  
Gene Expression ◽  
Differential Expression ◽  
Expression Patterns ◽  
Branching Morphogenesis ◽  
Computational Method ◽  
Metanephric Mesenchyme ◽  
Ureteric Bud ◽  
Cdna Arrays ◽  
Apoptotic Genes ◽  
Collecting System

Branching morphogenesis of the ureteric bud in response to unknown signals from the metanephric mesenchyme gives rise to the urinary collecting system and, via inductive signals from the ureteric bud, to recruitment of nephrons from undifferentiated mesenchyme. An established cell culture model for this process employs cells of ureteric bud origin (UB) cultured in extracellular matrix and stimulated with conditioned media (BSN-CM) from a metanephric mesenchymal cell line (H. Sakurai, E. J. Barros, T. Tsukamoto, J. Barasch, and S. K. Nigam. Proc. Natl. Acad. Sci. USA 94: 6279–6284, 1997.). In the presence of BSN-CM, the UB cells form branching tubular structures reminiscent of the branching ureteric bud. The pattern of gene regulation in this model of branching morphogenesis of the kidney collecting system was investigated using high-density cDNA arrays. Software and analytical methods were developed for the quantification and clustering of genes. With the use of a computational method termed “vector analysis,” genes were clustered according to the direction and magnitude of differential expression in n-dimensional log-space. Changes in gene expression in response to the BSN-CM consisted primarily of differential expression of transcription factors with previously described roles in morphogenesis, downregulation of pro-apoptotic genes accompanied by upregulation of anti-apoptotic genes, and upregulation of a small group of secreted products including growth factors, cytokines, and extracellular proteinases. Changes in expression are discussed in the context of a general model for epithelial branching morphogenesis. In addition, the cDNA arrays were used to survey expression of epithelial markers and secreted factors in UB and BSN cells, confirming the largely epithelial character of the former and largely mesenchymal character of the later. Specific morphologies (cellular processes, branching multicellular cords, etc.) were shown to correlate with the expression of different, but overlapping, genomic subsets, suggesting differences in morphogenetic mechanisms at these various steps in the evolution of branching tubules.

scholarly journals Differential expression of apoptotic genes PDIA3 and MAP3K5 distinguishes between low- and high-risk prostate cancer

2009 ◽  
Vol 8 (1) ◽  
pp. 130 ◽  
Author(s):  
Nicole Pressinotti ◽  
Helmut Klocker ◽  
Georg Schäfer ◽  
Van-Duc Luu ◽  
Markus Ruschhaupt ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
High Risk ◽  
Differential Expression ◽  
High Risk Prostate Cancer ◽  
Risk Prostate Cancer ◽  
Apoptotic Genes

scholarly journals P212A Mutant of Dihydrodaidzein Reductase Enhances (S)-Equol Production and Enantioselectivity in a Recombinant Escherichia coli Whole-Cell Reaction System

2016 ◽  
Vol 82 (7) ◽  
pp. 1992-2002 ◽  
Author(s):  
Pyung-Gang Lee ◽  
Joonwon Kim ◽  
Eun-Jung Kim ◽  
EunOk Jung ◽  
Bishnu Prasad Pandey ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Reaction System ◽  
Gut Bacteria ◽  
Site Directed Mutagenesis ◽  
Anaerobic Growth ◽  
Efficient Production ◽  
Cell Reaction ◽  
Whole Cell ◽  
Content Type ◽  
Postmenopausal Symptoms

ABSTRACT(S)-Equol, a gut bacterial isoflavone derivative, has drawn great attention because of its potent use for relieving female postmenopausal symptoms and preventing prostate cancer. Previous studies have reported on the dietary isoflavone metabolism of several human gut bacteria and the involved enzymes for conversion of daidzein to (S)-equol. However, the anaerobic growth conditions required by the gut bacteria and the low productivity and yield of (S)-equol limit its efficient production using only natural gut bacteria. In this study, the low (S)-equol biosynthesis of gut microorganisms was overcome by cloning the four enzymes involved in the biosynthesis fromSlackia isoflavoniconvertensintoEscherichia coliBL21(DE3). The reaction conditions were optimized for (S)-equol production from the recombinant strain, and this recombinant system enabled the efficient conversion of 200 μM and 1 mM daidzein to (S)-equol under aerobic conditions, achieving yields of 95% and 85%, respectively. Since the biosynthesis oftrans-tetrahydrodaidzein was found to be a rate-determining step for (S)-equol production, dihydrodaidzein reductase (DHDR) was subjected to rational site-directed mutagenesis. The introduction of the DHDR P212A mutation increased the (S)-equol productivity from 59.0 mg/liter/h to 69.8 mg/liter/h in the whole-cell reaction. The P212A mutation caused an increase in the (S)-dihydrodaidzein enantioselectivity by decreasing the overall activity of DHDR, resulting in undetectable activity for (R)-dihydrodaidzein, such that a combination of the DHDR P212A mutant with dihydrodaidzein racemase enabled the production of (3S,4R)-tetrahydrodaidzein with an enantioselectivity of >99%.

scholarly journals Bile acids and bilirubin effects on osteoblastic gene profile. Implications in the pathogenesis of osteoporosis in liver diseases

2019 ◽  
Author(s):  
Silvia Ruiz-Gaspà ◽  
Nuria Guañabens ◽  
Susana Jurado González ◽  
Marta Dubreuil ◽  
Andres Combalia ◽  
...  
Keyword(s):  
Differential Expression ◽  
Liver Diseases ◽  
Target Genes ◽  
Lithocholic Acid ◽  
Culture Conditions ◽  
Matrix Gla Protein ◽  
Osteoblastic Cells ◽  
Human Osteosarcoma Cells ◽  
Apoptotic Genes ◽  
End Stage

AbstractOsteoporosis in advanced cholestatic and end-stage liver disease is related to low bone formation. Previous studies have demonstrated the deleterious consequences of lithocholic acid (LCA) and bilirubin on osteoblastic cells. These effects are partially or completely neutralized by ursodeoxycholic acid (UDCA). We have assessed the differential gene expression of osteoblastic cells under different culture conditions. The experiments were performed in human osteosarcoma cells (Saos-2) cultured with LCA 10 μM), bilirubin (50 μM) or UDCA (10 and 100 μM) at 2 and 24 hours. Expression of 87 genes related to bone metabolism and other signalling pathways were assessed by TaqMan micro fluidic cards. Several genes were up-regulated by LCA, most of them pro-apoptotic (BAX, BCL10, BCL2L13, BCL2L14), but also MGP (matrix Gla protein), BGLAP (osteocalcin), SPP1 (osteopontin) and CYP24A1, and down-regulated bone morphogenic protein genes (BMP3 and BMP4) and DKK1 (Dickkopf-related protein 1). Parallel effects were observed with bilirubin, which up-regulated apoptotic genes and CSF2 (colony-stimulating factor 2) and down-regulated antiapoptotic genes (BCL2 and BCL2L1), BMP3, BMP4 and RUNX2. UDCA 100 μM had specific consequences since differential expression was observed, up-regulating BMP2, BMP4, BMP7, CALCR (calcitonin receptor), SPOCK3 (osteonectin), BGLAP (osteocalcin) and SPP1 (osteopontin), and down-regulating pro-apoptotic genes. Furthermore, most of the differential expression changes induced by both LCA and bilirubin were partially or completely neutralized by UDCA. Conclusion: Our observations reveal novel target genes, whose regulation by retained substances of cholestasis may provide additional insights into the pathogenesis of osteoporosis in cholestatic and end-stage liver diseases.

INCREASE: An in-cell reaction system for multi-nucleon transfer and spontaneous fission at the FRS ion catcher

2022 ◽  
Vol 512 ◽  
pp. 83-90
Author(s):  
Adrian Rotaru ◽  
Daler Amanbayev ◽  
Dimiter L. Balabanski ◽  
David Benyamin ◽  
Paul Constantin ◽  
...  
Keyword(s):  
Spontaneous Fission ◽  
Reaction System ◽  
Cell Reaction ◽  
Nucleon Transfer

Differential Expression of Long Noncoding RNAs During Cardiac Allograft Rejection

2017 ◽  
Vol 101 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Guangxiang Gu ◽  
Yingjuan Huang ◽  
Chenglin Wu ◽  
Zhiyong Guo ◽  
Yi Ma ◽  
...  
Keyword(s):  
Differential Expression ◽  
Allograft Rejection ◽  
Noncoding Rnas ◽  
Cardiac Allograft ◽  
Long Noncoding Rnas ◽  
Cardiac Allograft Rejection

EFFECT OF ANTI-INFLAMMATORY CYTOKINES ON THE ACTIVATION OF LYMPHOCYTES BY LACRIMAL GLAND ACINAR CELLS IN AN AUTOLOGOUS MIXED CELL REACTION.

Cornea ◽  
2000 ◽  
Vol 19 (Supplement 2) ◽  
pp. S133
Author(s):  
Melvin D. Trousdale ◽  
Douglas Stevenson ◽  
Harvey R. Kaslow ◽  
Joel E. Schechter ◽  
Dwight W. Warren ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Lacrimal Gland ◽  
Acinar Cells ◽  
Cell Reaction ◽  
Mixed Cell ◽  
Anti Inflammatory

DIFFERENTIAL EXPRESSION OF ADHESION MOLECULES DURING RAT SMALL BOWEL ALLOGRAFT REJECTION

1995 ◽  
Vol 60 (11) ◽  
pp. 989-992 ◽  
Author(s):  
STEVEN D. REID ◽  
CAROLINE R. UFF ◽  
IMRAN SAEED ◽  
JERARD ROSS ◽  
RICHARD P.M. WOOD ◽  
...  
Keyword(s):  
Small Bowel ◽  
Differential Expression ◽  
Adhesion Molecules ◽  
Allograft Rejection
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