scholarly journals OSCA: A one-step construction of adenovirus system using the Gibson DNA Assembly technology

2021 ◽  
Author(s):  
Na Ni ◽  
Fang Deng ◽  
Fang He ◽  
Hao Wang ◽  
Deyao Shi ◽  
...  
Keyword(s):  
Dna Assembly ◽  
One Step ◽  
Assembly Technology

scholarly journals Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Zheng Hu ◽  
Li Wang ◽  
Zhaoying Shi ◽  
Jing Jiang ◽  
Xiangning Li ◽  
...  
Keyword(s):  
Dna Assembly ◽  
Dna Templates ◽  
Core Fragment ◽  
Cas9 Nuclease ◽  
Overlap Extension ◽  
One Step ◽  
Dna Fragment ◽  
Polymerase Chain

Abstract Overlap extension polymerase chain reaction (PCR) is a powerful technology for DNA assembly. Based on this technology, we synthesized DNA templates, which were transcribed into sgRNA in vitro, and further detected their efficiency of purified sgRNAs with Cas9 nuclease. The sgRNAs synthesized by this approach can effectively cleave the DNA fragments of interest in vitro and in vivo. Compared with the conventional method for generating sgRNA, it does not require construction of recombinant plasmids and design of primers to amplify sgRNA core fragment. Only several short primers with overlapped sequences are needed to assemble a DNA fragment as the template of sgRNA. This modified and simplified method is highly applicable and less time-consuming.

scholarly journals Y la biología se convirtió en ingeniería: La adopción de estándares para sistemas vivos

2020 ◽  
Author(s):  
Victor De Lorenzo
Keyword(s):  
Synthetic Biology ◽  
Production Process ◽  
Industrial Production ◽  
Industrial Design ◽  
Dna Assembly ◽  
Fundamental Knowledge ◽  
Biological Objects ◽  
Activity Measurements ◽  
One Step ◽  
Design Construction

For decades, molecular biologists have been removing or inserting genes into all kinds of organisms with biotechnological intent or simply to generate fundamental knowledge. Synthetic biology (SynBio) goes one step further by incorporating conceptual frameworks from computing, electronics, and industrial design. This change makes it possible to conceive the creation of complex biological objects that were previously considered too difficult to assemble. To do this, the stages of any industrial production process must be adopted: design, construction of the components, assembly, and final manufacture. This objective requires standardisation of the physical and functional formats of the components involved, DNA assembly methods, activity measurements, and descriptive languages.

One step DNA assembly for combinatorial metabolic engineering

2014 ◽  
Vol 23 ◽  
pp. 70-77 ◽  
Author(s):  
Pieter Coussement ◽  
Jo Maertens ◽  
Joeri Beauprez ◽  
Wouter Van Bellegem ◽  
Marjan De Mey
Keyword(s):  
Metabolic Engineering ◽  
Dna Assembly ◽  
One Step

scholarly journals Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Kenji Tsuge ◽  
Yukari Sato ◽  
Yuka Kobayashi ◽  
Maiko Gondo ◽  
Masako Hasebe ◽  
...  
Keyword(s):  
Dna Assembly ◽  
One Step ◽  
Dna Mixture

An Interactive Minicomputer Program for the Indexing and Simulation of Electron Diffraction Patterns

1976 ◽  
Vol 34 ◽  
pp. 542-543
Author(s):  
R.P. Goehner ◽  
W.T. Hatfield ◽  
Prakash Rao
Keyword(s):  
Electron Diffraction ◽  
Real Time ◽  
Pattern Analysis ◽  
Flow Diagram ◽  
Hard Copy ◽  
Time Analysis ◽  
Real Time Analysis ◽  
Transmission Electron ◽  
One Step ◽  
Diffraction Patterns

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.

Nutrient-regulated gene expression in eukaryotes

2006 ◽  
Vol 73 ◽  
pp. 85-96 ◽  
Author(s):  
Richard J. Reece ◽  
Laila Beynon ◽  
Stacey Holden ◽  
Amanda D. Hughes ◽  
Karine Rébora ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcriptional Control ◽  
Direct Interaction ◽  
Signalling Pathways ◽  
A Cell ◽  
One Step ◽  
Higher Eukaryotes ◽  
Regulated Gene Expression

The recognition of changes in environmental conditions, and the ability to adapt to these changes, is essential for the viability of cells. There are numerous well characterized systems by which the presence or absence of an individual metabolite may be recognized by a cell. However, the recognition of a metabolite is just one step in a process that often results in changes in the expression of whole sets of genes required to respond to that metabolite. In higher eukaryotes, the signalling pathway between metabolite recognition and transcriptional control can be complex. Recent evidence from the relatively simple eukaryote yeast suggests that complex signalling pathways may be circumvented through the direct interaction between individual metabolites and regulators of RNA polymerase II-mediated transcription. Biochemical and structural analyses are beginning to unravel these elegant genetic control elements.

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