Association of VEGF-A genetic polymorphisms with cancer risk and survival in advanced-stage oral squamous cell carcinoma patients

AbstractOral squamous cell carcinoma is the third most common cancer in Indian subcontinent affecting people with lower socioeconomic status. Due to inadequate screening facilities and lack of awareness among individuals most of the oral cancer cases are detected at an advanced stage. As early stage oral squamous cell carcinoma patients can be treated with single modality treatment (surgery or radical radiotherapy), multimodality regimen (surgery followed by concurrent chemoradiation) is adopted for high risk advanced stage cancers with multiple adverse features like extra nodal extension, lymphovascular invasion and perineural spread. The chapter outlines the principles of adjunctive therapy in oral cancer patients with special reference to different techniques, indications of radiotherapy and role of chemotherapeutic regimes in improving the overall survival of advanced stage oral cancer patients.

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Lack of Association of CYP2C9 genetic polymorphism with oral squamous cell carcinoma

10.21203/rs.3.rs-27686/v1 ◽

2020 ◽

Author(s):

Sagheer Ahmed ◽

Saeed Khan ◽

Syeda Uzma Naqvi ◽

Kholood Janjua ◽

Zaira Zulfiqar ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Genetic Polymorphisms ◽

Squamous Cell ◽

Heterocyclic Aromatic Amines ◽

P Value ◽

Polycyclic Aromatic ◽

Allele Specific ◽

And Control

Abstract Background: There is increasing evidence for the role of polycyclic aromatic hydrocarbons (PAHs) and heterocyclic aromatic amines (HCAs) in carcinogenesis, including oral squamous cell carcinoma (OSCC). Several of these mutagenic substances are cytochrome (CYP)2C9 enzyme substrates.Methods: In this study, we examined the association of CYP2C9*2 and *3 genetic polymorphisms in 58 OSCC patients and 174 healthy, age, and sex-matched controls. Genotyping was done with allele-specific polymerase chain reaction followed by agarose gel electrophoreses, while selected samples were sequenced for confirmation of genotyping.Results: The wild type genotype (CYP2C9*1*1) was observed at 83%, *1*3 at 8%, *1*2 at 5%, *2*2 at 2% and *2*3 at 2% in combined case and control groups. On further analysis, however, our results did not reveal an association of these variants with OSCC samples (Odds ratio: 0.608, 95% Confidence Interval: 0.289 - 1.281, p-value: 0.190). While larger studies are needed to confirm or refute these results, they show a lack of association of CYP2C9*2 and *3 polymorphisms with OSCC in this population.Conclusions: This study demonstrates that the genetic polymorphisms in CYP2C9 genes (CYP2C9*2 and *3) are not causing the risk and are not associated with OSCC. Also, CYP2C9 has no role in the pathogenesis of OSCC in this population of patients.Trial registration: Not applicable.

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The interplay between oral microbiome, lifestyle factors and genetic polymorphisms in the risk of oral squamous cell carcinoma

Carcinogenesis ◽

10.1093/carcin/bgy053 ◽

2018 ◽

Vol 39 (6) ◽

pp. 778-787 ◽

Cited By ~ 28

Author(s):

Jenn-Ren Hsiao ◽

Chan-Chi Chang ◽

Wei-Ting Lee ◽

Cheng-Chih Huang ◽

Chun-Yen Ou ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Genetic Polymorphisms ◽

Squamous Cell ◽

Lifestyle Factors ◽

Oral Microbiome

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Genetic Polymorphisms ofADH1CandCYP2E1and Risk of Oral Squamous Cell Carcinoma

Otolaryngology ◽

10.1177/0194599811408778 ◽

2011 ◽

Vol 145 (4) ◽

pp. 586-593 ◽

Cited By ~ 11

Author(s):

Miroslav Brocic ◽

Gordana Supic ◽

Katarina Zeljic ◽

Nebojsa Jovic ◽

Ruzica Kozomara ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Genetic Polymorphisms ◽

Squamous Cell

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Difference in Clinical Presentation and Their Significance in Oral Squamous Cell Carcinoma – a Retrospective Analysis

10.21203/rs.3.rs-1159763/v1 ◽

2022 ◽

Author(s):

Jeyashanth Riju ◽

Amit Jiwan Tirkey ◽

Malavika Babu ◽

Ronald Anto ◽

Amey Madhav Baitule ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Perineural Invasion ◽

Clinical Presentation ◽

Tongue Cancer ◽

Clinical History ◽

Depth Of Invasion ◽

Advanced Stage

Abstract Oral squamous cell carcinoma(OSCC) involving tongue and buccoalveolar complex(BAC) behaves differently. Clinical features of the two subsites and their influence on pathological factors remain least analysed. Patients are divided into two groups i.e, tongue cancer and BAC cancer group, and various clinical parameters were compared. Among 474 patients 232 had tongue cancer and 242 had BAC cancer. 30% of patients with OSCC were asymptomatic at presentation except for the ulcer. Compared to tongue cancers, lesions confined to BAC presents at an advanced stage(p=0.006). Multivariate analysis showed that dysphagia in tongue cancer(p=0.020) and external swelling or lesion in BAC cancers(p=0.002) were significant predictors of an advanced stage of disease. On histopathology perineural invasion was significantly associated tongue(p=0.008) and BAC cancers(P=0.015). Among tongue cancers, those with pain and referred otalgia had a statistically significantly high depth of invasion(DOI), compared to those without pain (DOI – no pain 6.9mm, pain 9.9mm and referred otalgia 11.4mm). There is a delay in clinical presentation of OSCC. Among tongue cancers, clinical history of pain was significantly associated with depth of invasion and perineural invasion, the significance of which needs to be prospectively analysed. Clinical history in OSCC can be used as predicting factors for various pathological characters, which is subsite specific.

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Osteopontin Involves Cisplatin Resistance and Poor Prognosis in Oral Squamous Cell Carcinoma

BioMed Research International ◽

10.1155/2015/508587 ◽

2015 ◽

Vol 2015 ◽

pp. 1-13 ◽

Cited By ~ 6

Author(s):

Sheng-Dean Luo ◽

Yi-Ju Chen ◽

Chien-Ting Liu ◽

Kun-Ming Rau ◽

Yi-Ching Chen ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Cancer ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Locally Advanced ◽

Cancer Cell Line ◽

Poor Response ◽

Advanced Stage ◽

Cancer Line

Background.Osteopontin (OPN) is a multifunctional cytokine involved in cell survival, migration, and adhesion. However, its role in chemosensitivity in locally advanced oral squamous cell carcinoma (OSCC) in humans has not yet been investigated.Methods.We enrolled 121 patients with locally advanced stage IVA/B OSCC receiving cisplatin-based IC followed by CCRT from January 1, 2006, through January 1, 2012. Immunohistochemistry was used to assess OPN expression in OSCC patients’ biopsy specimens from paraffin blocks before treatment. In addition, MTT/colony formation assay was used to estimate the influence of OPN in an oral cancer cell line treated with cisplatin.Results.Of the 121 patients, 94 had positive OPN findings and 52 responded to IC followed by CCRT. Positive osteopontin immunostaining also correlated significantly with positive N status/TNM stage/male gender and smoking. Univariate analyses showed that patients whose tumors had a low expression of OPN were more likely to respond to chemotherapy and have a significantly better OS than those whose tumors had a high expression of OPN. Multivariate analysis revealed that prolonged survival was independently predicted for patients with stage IVA disease, negative lymph nodes, and negative expressions of OPN and for those who received chemotherapy with Docetaxel/cisplatin/fluorouracil (TPF). An oral cancer line stimulated with OPN exhibited a dose-dependent resistance to cisplatin treatment. Conversely, endogenous OPN depletion by OPN-mediated shRNA increased sensitivity to cisplatin.Conclusions.A positive expression of OPN predicts a poor response and survival in patients with locally advanced stage IVA/B OSCC treated with cisplatin-based IC followed by CCRT.

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Stat3 Expression in Oral Squamous Cell Carcinoma: Association with Clinicopathological Parameters and Survival

The International Journal of Biological Markers ◽

10.1177/172460080602100307 ◽

2006 ◽

Vol 21 (3) ◽

pp. 175-183 ◽

Cited By ~ 19

Author(s):

N.G. Shah ◽

T.I. Trivedi ◽

R.A. Tankshali ◽

J.A. Goswami ◽

D.H. Jetly ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Early Stage ◽

Disease Stage ◽

Clinicopathological Parameters ◽

Early Event ◽

Advanced Stage ◽

Intense Staining

The present study sought to explore the occurrence of signal transducer and activator of transcription 3 (Stat3) in patients with oral squamous cell carcinoma (n=135) and its potential relationship with clinicopathological parameters and survival. Stat3 expression was studied by immunohistochemistry. Cytoplasmic or nuclear localization of Stat3 was observed in 62% of patients, whereas only nuclear Stat3 expression was found in 44%. Stat3 positivity in early-stage patients was 45% compared to 79% in advanced-stage patients. However, early-stage Stat3-positive patients showed a gradual increase in staining intensity, with intense staining seen in 52% of the tumors compared to 18% in Stat3-positive advanced-stage patients, where a gradual decrease in intensity expression was observed (p=0.001). Stat3 showed a significant positive correlation with disease stage (p=0.001), nodal status (p=0.033) and tumor size (p=0.001). Multivariate survival analysis using the Cox proportional hazard regression model showed that nuclear Stat3 was a significant independent prognosticator for both relapse-free survival (p=0.014) and overall survival (p=0.042) in early-stage patients. Our results indicated that Stat3 activation is an early event in oral squamous cell carcinoma and represents a potential risk factor for poor prognosis in early-stage patients.

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