Serodiagnosis of Toxoplasma gondii infection in farm animals (horses, swine, and sheep) by enzyme-linked immunosorbent assay using chimeric antigens

This study was carried out in order to investigate the seroprevalence of Toxoplasma gondii infection in goats of seven different regions of District Dir (Lower), Pakistan. Lateral Flow Chromatographic Immunoassay (LFCI), Latex Agglutination Test (LAT) and Enzyme-Linked Immunosorbent Assay (ELISA) were used for the detection of T. gondii infection in goats. A total of 175 blood samples were collected from goats. Out of which 70 (40%) were found positive for T. gondii. The prevalence of toxoplasmosis among goats in seven areas of District Dir (Lower) was found high at Samarbagh (48%), Balambat (44%) followed by khall (40%), Munda (40%), LalQilla (36%) and lowest in Adenzai and Timergara (36%). This study demonstrated that the prevalence in older goats was significantly (P< 0.05) higher (48.91%) than younger ones (30.12%). The seroprevalence in male and female goats were 16 (29%) and 54 (45%) respectively.

Download Full-text

Serodiagnosis of Recently Acquired Toxoplasma gondii Infection Using an Enzyme-Linked Immunosorbent Assay with a Combination of Recombinant Antigens

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.7.5.781-787.2000 ◽

2000 ◽

Vol 7 (5) ◽

pp. 781-787 ◽

Cited By ~ 43

Author(s):

Shuli Li ◽

Gina Galvan ◽

Fausto G. Araujo ◽

Yasuhiro Suzuki ◽

Jack S. Remington ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Pregnant Women ◽

Immunosorbent Assay ◽

Immunoglobulin G ◽

Enzyme Linked Immunosorbent Assay ◽

Recombinant Antigens ◽

Single Antigen ◽

Toxoplasma Gondii Infection

ABSTRACT An enzyme-linked immunosorbent assay (ELISA) using four recombinant antigens of Toxoplasma gondii (rP22, rP25, rP29, and rP35) was used in an attempt to differentiate pregnant women with toxoplasma serologic profiles (TSPs) indicative of recently acquired infections (acute profile) from those with TSPs indicative of infections acquired in the distant past (chronic profile). In general, immunoglobulin G antibodies in sera from women with the acute profile reacted more strongly with the recombinant antigens than did those in sera from women with the chronic profile. However, reactivities differed significantly between antigens that reacted with a single serum and between sera that reacted with a single antigen. Because of these variations, we employed a combination of the four antigens in an ELISA (Comb-ELISA) and evaluated its ability to distinguish pregnant women with the acute profile from those with the chronic profile. Eighteen of 20 (90%) sera from acute-profile women were positive in the Comb-ELISA, whereas 69 of 70 (98.6%) sera from the chronic-profile women were negative. Thus, the Comb-ELISA may be useful for diagnosis of toxoplasmosis in pregnant women and for differentiation between recently acquired infections and infections acquired in the more distant past.

Download Full-text

Use of Protein AG in an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Toxoplasma gondii Infection in Four Species of Animals

Clinical and Vaccine Immunology ◽

10.1128/cvi.00479-09 ◽

2010 ◽

Vol 17 (3) ◽

pp. 485-486 ◽

Cited By ~ 17

Author(s):

Donglin Zhang ◽

Zhengsong Wang ◽

Rui Fang ◽

Hao Nie ◽

Huihui Feng ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Immunosorbent Assay ◽

Serological Diagnosis ◽

Enzyme Linked Immunosorbent Assay ◽

Toxoplasma Gondii Infection

ABSTRACT An enzyme-linked immunosorbent assay (ELISA) employing protein AG (AG-ELISA) as a conjugate was developed to detect anti-Toxoplasma gondii antibodies (Ab) in experimentally infected pigs and naturally infected pigs, goats, dogs, and cats. The results indicate that AG-ELISA can be a useful method for serological diagnosis of T. gondii infection in these four species of animals.

Download Full-text

Analysis of the antibodies anti-Toxoplasma gondii by ELISA based on two diagnostic antigens: rSAG1 and rBAG1

Acta Parasitologica ◽

10.2478/s11686-011-0082-2 ◽

2011 ◽

Vol 56 (4) ◽

Cited By ~ 3

Author(s):

Min Liu ◽

Tao Wang ◽

Hua Li ◽

Ju-Ying Li ◽

Han Zhong ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Immunosorbent Assay ◽

Blood Donors ◽

Enzyme Linked Immunosorbent Assay ◽

Igg Antibody ◽

Neuropsychiatric Disease ◽

Increased Risk ◽

Positive Rate ◽

Toxoplasma Gondii Infection ◽

Uncertain Etiology

AbstractSchizophrenia is a serious neuropsychiatric disease of uncertain etiology. Previous studies have demonstrated that antibodies to Toxoplasma gondii infection are associated with an increased risk of schizophrenia. The objective of this study was to analyze anti-T. gondii antibodies in 477 Chinese schizophrenia patients using an enzyme-linked immunosorbent assay (ELISA) based on recombinant surface antigen 1 (rSAG1), recombinant bradyzoite antigen 1 (rBAG1) and the soluble tachyzoite antigens (STAg) of T. gondii RH strain. Results showed that among the sero-positives (IgG and/or IgM) for T. gondii infection examined in schizophrenia patients, sero-positive samples for rSAG1, rBAG1 and STAg were 20.5% (98/477), 20.5% (98/477) and 23.5% (112/477) respectively, while compared to 210 blood donors, sero-positive (IgG and/or IgM) samples for these antigens (rSAG1, rBAG1 and STAg) were only 5.7% (12/210), 6.2% (13/210) and 5.7% (12/210), respectively. Furthermore, when IgG antibody reaction in the schizophrenia sera was compared with the rBAG1 and rSAG1, results demonstrated that beside the cases which can be detected by both rSAG1 and rBAG1, some sero-positive for T. gondii in schizophrenia sera can only be detected either by rSAG1 or rBAG1. This phenomenon was also observed in the detection of IgM with rSAG1 and rBAG1. 5.9% (28/477) of cases of schizophrenia which are positive for IgG or IgM by rSAG1 are negative for STAg, while 9.2% (44/477) of the schizophrenia cases which are positive for IgG or IgM by rBAG1 are negative for STAg. Although STAg can also be used to diagnose T. gondii infection from schizophrenia patients, it may not actually indicate the infection as some positive samples may be mistakenly considered to be negative. In conclusion, our results demonstrate that the sero-positive rate for T. gondii in the Chinese schizophrenia patients was higher than blood donors. More importantly, our results provide evidence that the combination of rSAG1 and rBAG1 antigens in the diagnosis of T. gondii infection could closely reflect the actual infection of this parasite in schizophrenia patients.

Download Full-text

Presence of anti-Toxoplasma antibodies in humans and their cats in the urban zone of Guadalajara

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86821999000500003 ◽

1999 ◽

Vol 32 (5) ◽

pp. 483-488 ◽

Cited By ~ 14

Author(s):

María de la Luz Galván Ramírez ◽

Guillermo Sánchez Vargas ◽

Marcos Vielma Sandoval ◽

Juan Luis Soto Mancilla

Keyword(s):

Risk Factors ◽

Toxoplasma Gondii ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Toxoplasma Infection ◽

Urban Zone ◽

Lack Of Control ◽

Iga Antibodies

Cats are the definitive hosts of Toxoplasma gondii. Infected cats excrete oocysts in their feces, infecting humans and other animals. The objective of the present study was to determine the presence of anti-Toxoplasma antibodies in cat owners and their pets, and determine if there was a relationship between Toxoplasma infection and humans who live with infected cats. IgG anti-Toxoplasma antibodies in sera of 59 cat owners were determined by enzyme-linked immunosorbent assay (ELISA), in 24 sera from their cats, IgG, IgM, and IgA antibodies were found using Burney's ELISA. Thirty-eight (64%) of 59 cat owners were positive to IgG anti-Toxoplasma. Seropositivity for cats was 70.8% IgG, 8.3% IgM, and 62.5% IgA. Cohabitation with cats infected by T. gondii, feeding with leftovers or raw viscera, and lack of control over how their feces were handled are risk factors conducive for humans to become infected by T. gondii.

Download Full-text

Potential linkage between Toxoplasma gondii Infection and physical education scores of College Students

10.1101/2020.10.20.346817 ◽

2020 ◽

Author(s):

Zhijin Sheng ◽

Yu Jin ◽

Yinan Du ◽

Xinlei Yan ◽

Yong Yao

Keyword(s):

College Students ◽

Physical Education ◽

Toxoplasma Gondii ◽

Protozoan Parasite ◽

Anhui Province ◽

Behavioral Changes ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

School Students ◽

Toxoplasma Gondii Infection

ABSTRACTObjectiveToxoplasma gondii is a worldwide protozoan parasite that could infect virtually all warm-blooded animals, including humans. Our study aimed to investigate the prevalence of T. gondii infection in college students at Anhui province, China. Moreover, growing studies demonstrated the association between T. gondii infection and host behavioral changes. We also studied the linkage between T. gondii and scores of college students.Methods2704 serum samples of medical school students attending physical education lessons were collected from September 2017 to September 2019 and evaluated for T. gondii IgG antibodies using an enzyme-linked immunosorbent assay (ELISA). We also analysed PE scores of T. gondii infected students and T. gondii uninfected students.ResultsThe overall seroprevalence of T. gondii was 11.5%. The main risk factors related to T. gondii infections were cat in the household and gardening or agriculture activity. Furthermore, in basketball group and football group, scores of T. gondii seropositive students were significantly higher than that of seronegative students, while in other sports there is no difference between scores of T. gondii infected students and T. gondii uninfected students.ConclusionThis is the first report of T. gondii seroprevalence in college students in Anhui province, China.

Download Full-text