scholarly journals Potential linkage between Toxoplasma gondii Infection and physical education scores of College Students

2020 ◽  
Author(s):  
Zhijin Sheng ◽  
Yu Jin ◽  
Yinan Du ◽  
Xinlei Yan ◽  
Yong Yao
Keyword(s):  
College Students ◽  
Physical Education ◽  
Toxoplasma Gondii ◽  
Protozoan Parasite ◽  
Anhui Province ◽  
Behavioral Changes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
School Students ◽  
Toxoplasma Gondii Infection

ABSTRACTObjectiveToxoplasma gondii is a worldwide protozoan parasite that could infect virtually all warm-blooded animals, including humans. Our study aimed to investigate the prevalence of T. gondii infection in college students at Anhui province, China. Moreover, growing studies demonstrated the association between T. gondii infection and host behavioral changes. We also studied the linkage between T. gondii and scores of college students.Methods2704 serum samples of medical school students attending physical education lessons were collected from September 2017 to September 2019 and evaluated for T. gondii IgG antibodies using an enzyme-linked immunosorbent assay (ELISA). We also analysed PE scores of T. gondii infected students and T. gondii uninfected students.ResultsThe overall seroprevalence of T. gondii was 11.5%. The main risk factors related to T. gondii infections were cat in the household and gardening or agriculture activity. Furthermore, in basketball group and football group, scores of T. gondii seropositive students were significantly higher than that of seronegative students, while in other sports there is no difference between scores of T. gondii infected students and T. gondii uninfected students.ConclusionThis is the first report of T. gondii seroprevalence in college students in Anhui province, China.

scholarly journals Potential linkage between Toxoplasma gondii infection and physical education scores of college students

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0241653
Author(s):  
Jun Cai ◽  
Zhijin Sheng ◽  
Yu Jin ◽  
Yinan Du ◽  
Xinlei Yan ◽  
...  
Keyword(s):  
Risk Factors ◽  
College Students ◽  
Physical Education ◽  
Toxoplasma Gondii ◽  
Protozoan Parasite ◽  
Anhui Province ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
School Students ◽  
Toxoplasma Gondii Infection

Objective Toxoplasma gondii is a worldwide protozoan parasite that could infect virtually all warm-blooded animals, including humans. Our study aimed to investigate the prevalence of T. gondii infection in college students at Anhui province, China and to assess risk factors for T. gondii infection in college students. Moreover, growing studies demonstrated the association between T. gondii infection and host behavioral changes. We also studied the linkage between T. gondii and physical education (PE) scores of college students. Methods A total of 2704 serum samples of medical school students attending physical education lessons were collected from September 2017 to September 2019 and evaluated for T. gondii IgG antibodies using an enzyme-linked immunosorbent assay (ELISA). Questionnaires and statistical analysis were used to determine the risk factors for T. gondii infection. We also analysed PE scores of T. gondii -infected students and T. gondii-uninfected students. Results The overall seroprevalence of T. gondii was 11.5%. The main risk factors related to T. gondii infection in college students were cat in the household and gardening or agriculture activity. Furthermore, in the basketball group and the soccer group, scores of T. gondii seropositive students were significantly higher than those of seronegative students, while in other sports there was no difference between scores of T. gondii-infected students and T. gondii uninfected students. Conclusion This is the first report of T. gondii seroprevalence in college students in Anhui province, China.

scholarly journals Seroepidemiology of Toxoplasma gondii infection in patients with liver disease in eastern China

2017 ◽  
Vol 145 (11) ◽  
pp. 2296-2302 ◽  
Author(s):  
A. L. TIAN ◽  
G. X. LI ◽  
H. M. ELSHEIKHA ◽  
D. S. GARDNER ◽  
X. Y. ZHANG ◽  
...  
Keyword(s):  
Risk Factors ◽  
Liver Disease ◽  
Toxoplasma Gondii ◽  
Eastern China ◽  
Multivariate Logistic Regression Analysis ◽  
Protozoan Parasite ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Toxoplasma Gondii Infection

SUMMARYThe role of the protozoan parasite Toxoplasma gondii in the pathogenesis of liver disease has recently gained much interest. The aim of this study was to determine the prevalence and risk factors associated with T. gondii infection in patients with liver disease from three cities in Shandong and Henan provinces, China. A case–control study was conducted from December 2014 to November 2015 and included 1142 patients with liver disease and 1142 healthy controls. Serum samples were collected from all individuals and were examined with enzyme-linked immunosorbent assay for the presence of anti-T. gondii IgG and IgM antibodies. Information on the demographics, clinical, and lifestyle characteristics of the participants was collected from the medical records and by the use of a questionnaire. The prevalence of anti-T. gondii IgG was 19·7% in patients with liver disease compared with 12·17% in the controls. Only 13 patients had anti-T. gondii IgM antibodies compared with 12 control individuals (1·14% vs. 1·05%, respectively). The highest seroprevalence was detected in patients with liver cancer (22·13%), followed by hepatitis patients (20·86%), liver cirrhosis patients (20·42%), and steatosis patients (20%). Multivariate logistic regression analysis indicated that consumption of raw meat (odds ratio (OR) = 1·32; 95% confidence interval (CI) 1·01–1·71; P = 0·03) and source of drinking water from wells (OR = 1·56; 95% CI 1·08–2·27; P = 0·01) were independent risk factors for T. gondii infection in liver disease patients. These findings indicate that T. gondii infection is more likely to be present in patients with liver disease. Therefore, efforts should be directed toward health education of populations at high risk of T. gondii infection and measures should be taken to protect vulnerable patients with liver disease.

scholarly journals Use of MAG1 Recombinant Antigen for Diagnosis of Toxoplasma gondii Infection in Humans

2007 ◽  
Vol 14 (3) ◽  
pp. 220-225 ◽  
Author(s):  
Lucyna Holec ◽  
Elżbieta Hiszczyńska-Sawicka ◽  
Artur Gąsior ◽  
Anna Brillowska-Dąbrowska ◽  
Józef Kur
Keyword(s):  
Toxoplasma Gondii ◽  
Diagnostic Tests ◽  
Expression System ◽  
Recombinant Antigen ◽  
Acute Stage ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Acute Toxoplasmosis ◽  
Toxoplasma Gondii Infection ◽  
Potential Use

ABSTRACT This paper describes the cloning, purification, and serological applications of matrix antigen MAG1 of Toxoplasma gondii. The expression system used allows the production of a large amount of T. gondii recombinant protein, which was assessed for its potential use in an enzyme-linked immunosorbent assay (ELISA) for detection of T. gondii infection in humans. Serum samples from 117 patients with different stages of infection, along with 10 serum samples from seronegative patients obtained for routine diagnostic tests, were used. The results were compared with those of an ELISA that uses a native T. gondii antigen extract. The MAG1 antigen detected antibodies more frequently from the acute stage (97.3%) than from the chronic stage (7.5%) of toxoplasmosis. Hence, this antigen may be used as a tool for detection of T. gondii immunoglobulin G antibodies in persons with acute toxoplasmosis.

scholarly journals Seroprevalence of Toxoplasma gondii infection in sheep in Inner Mongolia Province, China

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 11 ◽  
Author(s):  
Xinlei Yan ◽  
Wenying Han ◽  
Yang Wang ◽  
Hongbo Zhang ◽  
Zhihui Gao
Keyword(s):  
Toxoplasma Gondii ◽  
Inner Mongolia ◽  
Animal Husbandry ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Zoonotic Parasite ◽  
Health And Disease ◽  
Toxoplasma Gondii Infection ◽  
Almost All

Toxoplasma gondii is an important zoonotic parasite that can infect almost all warm-blooded animals, including humans, and infection may result in many adverse effects on animal husbandry production. Animal husbandry in Inner Mongolia is well developed, but data on T. gondii infection in sheep are lacking. In this study, we determined the seroprevalence and risk factors associated with the seroprevalence of T. gondii using an indirect enzyme-linked immunosorbent assay (ELISA) test. A total of 1853 serum samples were collected from 29 counties of Xilin Gol League (n = 624), Hohhot City (n = 225), Ordos City (n = 158), Wulanchabu City (n = 144), Bayan Nur City (n = 114) and Hulunbeir City (n = 588). The overall seroprevalence of T. gondii was 15.43%. Risk factor analysis showed that seroprevalence was higher in sheep ≥12 months of age (21.85%) than that in sheep <12 months of age (10.20%) (p < 0.01). Seroprevalence was higher in male sheep (18.76%) than females (12.80%) (p < 0.01). Barn-feeding sheep (23.13%) had higher prevalence than grazing sheep (10.94%) (p < 0.01). The seroprevalence was significantly different in different districts (p < 0.01). This study shows that sheep are exposed to T. gondii in Inner Mongolia, and provides a data reference for public health and disease control.

Serological Survey and Associated Risk Factors on Toxoplasma gondii Infection in Goats in Mila District, Algeria

2020 ◽  
Vol 64 (1) ◽  
pp. 48-59 ◽  
Author(s):  
A. Dahmane ◽  
S. Boussena ◽  
F. Hafsi ◽  
F. Ghalmi
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Protozoan Parasite ◽  
Latex Agglutination ◽  
Enzyme Linked Immunosorbent Assay ◽  
Seroprevalence Rate ◽  
Serum Samples ◽  
Serological Tests ◽  
North Eastern ◽  
Eastern Algeria

AbstractToxoplasma gondii is a protozoan parasite prevalent in humans and other animals worldwide having medical and veterinary importance on account of reproductive failure causing significant socioeconomic losses. The aims of this study were to estimate the seroprevalence of T. gondii infection in goats, determined the possible risk factors associated, and evaluate the performances of the latex agglutination test (LAT) to anti-T. gondii antibodies screening using the indirect Enzyme-linked immunosorbent assay as a reference test (iELISA). A total of 184 serum samples from goats reared on 25 farms in Mila district from North-Eastern Algeria were collected and tested for anti-T. gondii IgG antibodies using two commercial serological tests (ELISA and LAT). A seroprevalence rate of 71.73 % and 63.58 % was obtained by both ELISA and LAT tests, respectively. The analysis of some factors thought to be related to the onset of this infection such as age, sex, management system, locality and presence of cats showed no significant relationship (P > 0.05); these factors did not seem to affect the frequency of the infection. The seropositivity level of T. gondii was significantly higher in aborted goats (P = 0.007), which suggested that they may play a significant role in pregnancy failure. In the concordance evaluation between the two serological tests (ELISA and LAT), the Cohen’s Kappa value was calculated and the results showed a K of 0.519 (p = 0.000) belonging to the range of 0.41—0.60 indicating just average agreement. The results of the Mc Nemar test showed that both tests gave significantly different results and seropositivity values (P < 0.05). The high prevalence observed in this study indicated a widespread exposure to T. gondii from goats and the potential risk of T. gondii infection for humans in North-Eastern Algeria. These results elucidate the challenges of applying serology to estimate goat exposure to T. gondii. The choice between the two serological tests will depend on their performances, as well as the availability of the equipment, laboratory conditions and the number of samples to be tested.

scholarly journals Seroprevalence of Toxoplasma gondii infection among indian people living in Iauareté, São Gabriel da Cachoeira, Amazonas, Brazil

2008 ◽  
Vol 50 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Márcio Neves Bóia ◽  
Filipe Anibal Carvalho-Costa ◽  
Fernando Campos Sodré ◽  
Gloria Maria Trindade Pinto ◽  
Maria Regina Reis Amendoeira
Keyword(s):  
Toxoplasma Gondii ◽  
Demographic Data ◽  
Age Groups ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Indian People ◽  
High Prevalence ◽  
Toxoplasma Gondii Infection

The goal of this survey was to estimate the seroprevalence of Toxoplasma gondii infection in Iauareté, a multiethnic Indian community in the upper Rio Negro basin. We carried out a cross-sectional survey (n = 260), in order to obtain serum samples and demographic data. The sample was randomly selected, by family conglomerate analysis. Serodiagnosis was performed by an enzyme-linked immunosorbent assay and indirect immunofluorescence. Prevalence of reactivity was 73.5% (191/260), being higher in the older-age groups, reaching 95.7% (44/46) in the group aged 50 years or more. The majority of seropositive subjects had titers equal to or less than 1:64. Seroprevalence was greater in Indians belonging to the Hupda ethnic group (p = 0.03). According to the present survey, Indian people living in Iauareté have a high prevalence of antibodies to T. gondii. Demographic concentration and urbanization within low sanitation and poor hygiene backgrounds, as well as unfiltered water consumption, may be related to the high frequency of T. gondii seroprevalence observed in the studied area.

scholarly journals Serological Studied On Toxoplasma Gondii Infection in Sheep and Goats in Ismailia Province

2018 ◽  
Vol 2 (2) ◽  
Keyword(s):  
Toxoplasma Gondii ◽  
Blood Serum ◽  
Elisa Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reference Test ◽  
Serum Samples ◽  
Sheep And Goats ◽  
Modified Agglutination Test ◽  
Toxoplasma Gondii Infection

Blood serum samples from 100 sheep and 100 goats were collected and examined for Toxoplasma gondii antibodies by Enzyme Linked Immunosorbent Assay and Modified Agglutination Test. The seroprevalences of Toxoplasma gondii in sheep were 34% and 33% and in goats were 32%, 31% by ELISA and MAT respectively. The prevalence in the females of sheep and goats were higher than males. The seroprevalences were higher in adult animals than young in both sheep and goats. Using the MAT as a reference test, the sensitivity and specificity of the ELISA Test were 100% and 98.5% respectively

scholarly journals Seroprevalence and associated risk factors of Toxoplasma gondii infection in yaks (Bos grunniens) on the Qinghai–Tibetan Plateau of China

Parasite ◽  
2021 ◽  
Vol 28 ◽  
pp. 43
Author(s):  
Tao Sun ◽  
Sajid Ur Rahman ◽  
Jinzhong Cai ◽  
Jiangyong Zeng ◽  
Rongsheng Mi ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Tibetan Plateau ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Bos Grunniens ◽  
Tibet Autonomous Region ◽  
Toxoplasma Gondii Infection ◽  
Associated Risk Factors ◽  
The Tibet Autonomous Region

Toxoplasma gondii is an intracellular parasite that is extensively prevalent globally. Studies have indicated the presence of T. gondii infection in animals in some provinces of China, but little is known about T. gondii infection in yaks (Bos grunniens) on the Qinghai–Tibetan Plateau. In the current study, to determine the seroprevalence and associated risk factors of T. gondii, a total of 2784 serum samples were collected from 18 different sampling sites in eight counties of the Qinghai and Tibet regions of China from 2018 to 2019. Serum antibodies against T. gondii were detected in 261 yaks (9.38%) via enzyme-linked immunosorbent assay (ELISA). We found that seroprevalence differed significantly among different counties (ranging from 5.41% in Gangcha to 19.79% in Datong), by year in the Tibet Autonomous Region (from 2.34% in 2018 to 13.24% in 2019), and by age (from 5.59% in 0 < year ≤ 1 to 11.76% in year > 7) (p < 0.05). Climate, geographical conditions, and age are the main factors influencing T. gondii infection in yaks in these regions. Therefore, our study provides a data reference for public health and prevention of yak toxoplasmosis.

scholarly journals Seroprevalence of Toxoplasma gondii and associated risk factors in domestic pigs raised from Cuba

2021 ◽  
Author(s):  
Julio César Castillo-Cuenca ◽  
Álvaro Martínez-Moreno ◽  
José Manuel Diaz-Cao ◽  
Angel Entrena-García ◽  
Jorge Fraga ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Meat Products ◽  
Control Measures ◽  
Health Concern ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Cross Sectional ◽  
Weaning Pigs ◽  
Associated Risk Factors

AbstractA cross-sectional study was carried out to determine the seroprevalence of Toxoplasma gondii and associated risk factors in pigs in the largest pork-producing region in Cuba. Serum samples from 420 pigs, including 210 sows and 210 post-weaning pigs, were tested for antibodies against T. gondii using a commercial indirect enzyme-linked immunosorbent assay. Anti-T. gondii antibodies were detected in 56 animals (13.3%, 95% CI: 10.1–16.6). A generalized estimating equations model revealed that the risk factors associated with higher seropositivity in pigs were altitude (higher in farm’s location < 250 m above sea level (masl) versus ≥ 250 masl) and age (higher in sows compared to post-weaning pigs). The results indicated that this protozoan parasite is widely distributed on pig farms in the study area, which is a public health concern since the consumption of raw or undercooked pork meat products containing tissue cysts is considered one of the main routes of T. gondii transmission worldwide. Control measures should be implemented to reduce the risk of exposure to T. gondii in pigs in Cuba.

scholarly journals Complete Protection against Lethal Toxoplasma gondii Infection in Mice Immunized with a Plasmid Encoding theSAG1 Gene

1999 ◽  
Vol 67 (12) ◽  
pp. 6358-6363 ◽  
Author(s):  
Henrik Vedel Nielsen ◽  
Sanne Lise Lauemøller ◽  
Lone Christiansen ◽  
Søren Buus ◽  
Anders Fomsgaard ◽  
...  
Keyword(s):  
T Cells ◽  
Toxoplasma Gondii ◽  
Cytotoxic T Lymphocyte ◽  
Protozoan Parasite ◽  
Complete Protection ◽  
Effective Protection ◽  
Partial Protection ◽  
Empty Plasmid ◽  
Toxoplasma Gondii Infection ◽  
Best Fit

ABSTRACT Infection with the protozoan parasite Toxoplasma gondiiis transmitted to humans from infected animals by tissue cysts and oocysts excreted by cats. Immunization with inactivated parasites or recombinant proteins has at best shown partial protection. We constructed a plasmid expressing the SAG1 surface antigen of T. gondii, p1tPASAG1, and showed that animals immunized with the plasmid produce anti-SAG1 antibodies which recognize the native SAG1. Mice immunized with p1tPASAG1 showed 80 to 100% protection against challenge with the non-cyst-producing, virulent RH isolate, compared to an 80% mortality in mice immunized with empty plasmid, which is the greatest efficacy of any vaccine against T. gondii produced so far. The SAG1 molecule was analyzed for potential cytotoxic T-lymphocyte (CTL) epitopes, and four peptides with the best fit were synthesized. The ability of the peptides to stimulate gamma interferon production by CD8+ T cells from p1tPASAG1-immunized mice was tested in an ELISPOT assay, and one new CTL epitope was identified. Adoptive transfer of CD8+ T cells from p1tPASAG1-immunized to naı̈ve mice showed partial protection. In conclusion, DNA vaccination with p1tPASAG1 gave effective protection in mice againstT. gondii infection and the protection could be adoptively transferred by purified CD8+ T cells.

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