scholarly journals The prognostic impact of CD7 expression of leukaemic blasts in de novo intermediate cytogenetic risk acute myeloid leukaemia

Pathology ◽  
2016 ◽  
Vol 48 ◽  
pp. S99
Author(s):  
Murali Kesavan ◽  
Hun Chuah ◽  
S.Aqif Mukhtar ◽  
Ashier Leigh Parsons ◽  
Kerryn Stoner ◽  
...  
Keyword(s):  
Acute Myeloid Leukaemia ◽  
Myeloid Leukaemia ◽  
De Novo ◽  
Prognostic Impact ◽  
Acute Myeloid

scholarly journals The Clinical Features and Prognosis of NPM1/FLT3-ITD/DNMT3A Triple-mutated Acute Myeloid Leukaemia

2021 ◽  
Author(s):  
Xingchun Luo ◽  
Bei Liu ◽  
Haiping Liang ◽  
Long Zhao ◽  
Yuancheng Guo ◽  
...  
Keyword(s):  
Acute Myeloid Leukaemia ◽  
Myeloid Leukaemia ◽  
Bioinformatics Analysis ◽  
De Novo ◽  
Prognostic Significance ◽  
Gene Expression Omnibus ◽  
Prognostic Impact ◽  
Newly Diagnosed ◽  
De Novo Aml ◽  
Acute Myeloid

Abstract Purpose: Previous studies have shown that patients with NPM1+/FLT3-ITD+ acute myeloid leukaemia (AML) have a poor prognosis, especially those with a high FLT3-ITD allelic ratio. However, no studies have confirmed a clear prognostic impact of DNMT3A on NPM1+/FLT3-ITD+ AML patients. Methods: Our study included a total of 165 patients with newly diagnosed non-acute promyelocytic leukaemia (non-APL) AML at The First Hospital of Lanzhou University between January 2018 and June 2021. Further bioinformatics analysis was performed using the Gene Expression Omnibus (GEO) database. Results: We retrospectively studied 165 patients newly diagnosed non-APL AML and identified 11 (6.7%) patients with NPM1/FLT3-ITD/DNMT3A triple mutations. The patients with triple-mutated AML had advanced age, higher white blood cell (WBC) counts, de novo AML, normal karyotypes, and poor survival, and all were in the M4/M5 French-American-British (FAB) category. Notably, half of the patients with triple-mutated AML had mature monocyte characteristics that were difficult to distinguish from chronic myelomonocytic leukaemia (CMML). We validated the prognosis of patients with triple-mutated AML by further bioinformatics analysis and found that the GNG4 gene, one of the hub genes, was related to triple-mutated AML patients' survival. Conclusion: Our data demonstrate that DNMT3A gene mutation has adverse prognostic significance in NPM1+FLT3-ITD+comutation AML patients.

Immunohistochemical detection of cytoplasmic nucleophosmin in formalin-fixed paraffin-embedded marrow trephine biopsies in acute myeloid leukaemia

2015 ◽  
Vol 69 (5) ◽  
pp. 409-414
Author(s):  
Benny Man Wai Lit ◽  
Yok Lam Kwong ◽  
Kit Fai Wong
Keyword(s):  
Bone Marrow ◽  
Acute Myeloid Leukaemia ◽  
Myeloid Leukaemia ◽  
Predictive Value ◽  
De Novo ◽  
Gene Mutations ◽  
Prognostic Impact ◽  
Direct Dna Sequencing ◽  
Formalin Fixed ◽  
Acute Myeloid

AimsNucleophosmin (NPM1) gene mutations resulting in cytoplasmic delocalisation of nucleophosmin (NPMc+) are the most common genetic abnormality in acute myeloid leukaemia (AML). In this study, we tested whether immunohistochemical (IHC) detection of cytoplasmic NPM1 (cNPM1) in formalin-fixed bone marrow trephine biopsies correlated with NPM1 mutations and the prognostic impact of NPM1 and fms-related tyrosine kinase 3-internal tandem duplication (FLT3-ITD) gene mutations was also assessed.MethodsA total of 71 Chinese adult de novo AML cases were evaluated for cNPM1 by IHC where the bone marrow trephines were fixed in 10% buffered formalin and decalcified by 5% EDTA. NPM1 and FLT3-ITD gene mutations were also investigated using PCR, fragment analysis and direct DNA sequencing.ResultsIHC analysis of cNPM1 had a very good sensitivity (86.7%) and excellent specificity (96.4%) for NPM1 mutation. The positive predictive value was 86.7% and the negative predictive value was 96.4%. NPM1 mutations and FLT3-ITD were closely associated (p=0.003). Patients with mutated NPM1 and without FLT3-ITD mutation have a longer overall survival (p=0.042) than patients with both NPM1 and FLT3-ITD mutations.ConclusionsOur results showed that IHC detection of cNPM1 in formalin-fixed trephine biopsies correlated well but not entirely with NPM1 mutation. Furthermore, NPM1 mutations were significantly more frequent in FLT3-ITD than FLT3-wild-type cases.

scholarly journals Adhesion Deregulation in Acute Myeloid Leukaemia

Cells ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 66 ◽  
Author(s):  
Alicja Gruszka ◽  
Debora Valli ◽  
Cecilia Restelli ◽  
Myriam Alcalay
Keyword(s):  
Cell Adhesion ◽  
Acute Myeloid Leukaemia ◽  
Myeloid Leukaemia ◽  
Epithelial To Mesenchymal Transition ◽  
Chemotherapeutic Agents ◽  
Prognostic Impact ◽  
Mesenchymal Transition ◽  
Haematopoietic Cells ◽  
Variable Extent ◽  
Acute Myeloid

Cell adhesion is a process through which cells interact with and attach to neighboring cells or matrix using specialized surface cell adhesion molecules (AMs). Adhesion plays an important role in normal haematopoiesis and in acute myeloid leukaemia (AML). AML blasts express many of the AMs identified on normal haematopoietic precursors. Differential expression of AMs between normal haematopoietic cells and leukaemic blasts has been documented to a variable extent, likely reflecting the heterogeneity of the disease. AMs govern a variety of processes within the bone marrow (BM), such as migration, homing, and quiescence. AML blasts home to the BM, as the AM-mediated interaction with the niche protects them from chemotherapeutic agents. On the contrary, they detach from the niches and move from the BM into the peripheral blood to colonize other sites, i.e., the spleen and liver, possibly in a process that is reminiscent of epithelial-to-mesenchymal-transition in metastatic solid cancers. The expression of AMs has a prognostic impact and there are ongoing efforts to therapeutically target adhesion in the fight against leukaemia.

scholarly journals Normal myeloid progenitor cell subset-associated gene signatures for acute myeloid leukaemia subtyping with prognostic impact

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0229593 ◽  
Author(s):  
Anna A. Schönherz ◽  
Julie Støve Bødker ◽  
Alexander Schmitz ◽  
Rasmus Froberg Brøndum ◽  
Lasse Hjort Jakobsen ◽  
...  
Keyword(s):  
Acute Myeloid Leukaemia ◽  
Myeloid Leukaemia ◽  
Progenitor Cell ◽  
Cell Subset ◽  
Prognostic Impact ◽  
Gene Signatures ◽  
Myeloid Progenitor ◽  
Myeloid Progenitor Cell ◽  
Acute Myeloid
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